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BCDA  (Synonyms: 5-bromo-4-chloroindoxyl acetate)

Cat. No.: HY-52112 Purity: 99.80%
COA
SDS
Handling Instructions Technical Support

BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase.

For research use only. We do not sell to patients.

BCDA

BCDA Chemical Structure

CAS No. : 3252-36-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 mg In-stock
50 mg In-stock
100 mg In-stock
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

BCDA Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BCDA

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase[1].

In Vitro

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of stock solution and working solution Prepare a high-concentration stock solution to dilute into the working buffer. Dissolve BCDA in a suitable solvent, such as DMF or DMSO. The typical concentration of the stock solution is 10-50 mg/mL. Buffer: Prepare a suitable buffer for the esterase (for example, 50 mM Tris-HCl, pH 7.5). Dilution: Dilute the stock solution into the buffer, with the final concentration being 0.1-1 mg/mL (or approximately 0.5-5 mM). 2. Detection method 2.1 Incubation: Incubate the sample at 37°C or room temperature. 2.2 Visualization: The esterase activity will convert the substrate into a blue precipitate. 2.3 Monitoring: Monitor the development of color. The reaction time depends on the enzyme activity, usually ranging from 30 minutes to several hours.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BCDA Related Antibodies
Molecular Weight

288.53

Formula

C10H7BrClNO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

BrC1=C(Cl)C2=C(NC=C2OC(C)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

Ethanol : ≥ 100 mg/mL (346.58 mM)

DMSO : 100 mg/mL (346.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4658 mL 17.3292 mL 34.6584 mL
5 mM 0.6932 mL 3.4658 mL 6.9317 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.80%

SDS (393 KB)

COA (252 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.4658 mL 17.3292 mL 34.6584 mL 86.6461 mL
5 mM 0.6932 mL 3.4658 mL 6.9317 mL 17.3292 mL
10 mM 0.3466 mL 1.7329 mL 3.4658 mL 8.6646 mL
15 mM 0.2311 mL 1.1553 mL 2.3106 mL 5.7764 mL
20 mM 0.1733 mL 0.8665 mL 1.7329 mL 4.3323 mL
25 mM 0.1386 mL 0.6932 mL 1.3863 mL 3.4658 mL
30 mM 0.1155 mL 0.5776 mL 1.1553 mL 2.8882 mL
40 mM 0.0866 mL 0.4332 mL 0.8665 mL 2.1662 mL
50 mM 0.0693 mL 0.3466 mL 0.6932 mL 1.7329 mL
60 mM 0.0578 mL 0.2888 mL 0.5776 mL 1.4441 mL
80 mM 0.0433 mL 0.2166 mL 0.4332 mL 1.0831 mL
100 mM 0.0347 mL 0.1733 mL 0.3466 mL 0.8665 mL
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BCDA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCDA3252-36-65-bromo-4-chloroindoxylFluorescent DyeChromogenic substrateesteraseInhibitorinhibitorinhibit

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