BCDA
Based on 1 publication(s) in Google Scholar
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase.
For research use only. We do not sell to patients.
- Purity : 99.80%
- CAS No.: 3252-36-6
- Formula: C10H7BrClNO2
- Molecular Weight:288.53
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-W008253
5-Hydroxyindole-3-acetic acid -
HY-W008253R
5-Hydroxyindole-3-acetic acid (Standard) -
HY-W016784
Indole-3-acetamide -
HY-W016784R
Indole-3-acetamide (Standard) -
HY-15934
X-GAL -
HY-18569
3-Indoleacetic acid -
HY-W015229
3-Indolepropionic acid -
HY-DY2002
X-GAL (solution) -
HY-59233
6-Bromoindole -
HY-30236
5-Bromoindole -
HY-W007384
5-Chloroindole -
HY-W002687
7-Bromo-1H-indole
Publications Citing Use of MedChemExpress (MCE) BCDA
More
Biological Activity
Description
In Vitro
Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of stock solution and working solution
Prepare a high-concentration stock solution to dilute into the working buffer.
Dissolve BCDA in a suitable solvent, such as DMF or DMSO. The typical concentration of the stock solution is 10-50 mg/mL.
Buffer: Prepare a suitable buffer for the esterase (for example, 50 mM Tris-HCl, pH 7.5).
Dilution: Dilute the stock solution into the buffer, with the final concentration being 0.1-1 mg/mL (or approximately 0.5-5 mM).
2. Detection method
2.1 Incubation: Incubate the sample at 37°C or room temperature.
2.2 Visualization: The esterase activity will convert the substrate into a blue precipitate.
2.3 Monitoring: Monitor the development of color. The reaction time depends on the enzyme activity, usually ranging from 30 minutes to several hours.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 3252-36-6
-
Appearance Solid
-
Molecular Weight 288.53
-
Formula C10H7BrClNO2
-
Color White to off-white
-
SMILES
BrC1=C(Cl)C2=C(NC=C2OC(C)=O)C=C1
-
Synonyms
5-bromo-4-chloroindoxyl acetate
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
Solvent & Solubility
In Vitro:
Ethanol : ≥ 100 mg/mL (346.58 mM)
DMSO : 100 mg/mL (346.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 3.4658 mL | 17.3292 mL | 34.6584 mL | 86.6461 mL |
| 5 mM | 0.6932 mL | 3.4658 mL | 6.9317 mL | 17.3292 mL | |
| 10 mM | 0.3466 mL | 1.7329 mL | 3.4658 mL | 8.6646 mL | |
| 15 mM | 0.2311 mL | 1.1553 mL | 2.3106 mL | 5.7764 mL | |
| 20 mM | 0.1733 mL | 0.8665 mL | 1.7329 mL | 4.3323 mL | |
| 25 mM | 0.1386 mL | 0.6932 mL | 1.3863 mL | 3.4658 mL | |
| 30 mM | 0.1155 mL | 0.5776 mL | 1.1553 mL | 2.8882 mL | |
| 40 mM | 0.0866 mL | 0.4332 mL | 0.8665 mL | 2.1662 mL | |
| 50 mM | 0.0693 mL | 0.3466 mL | 0.6932 mL | 1.7329 mL | |
| 60 mM | 0.0578 mL | 0.2888 mL | 0.5776 mL | 1.4441 mL | |
| 80 mM | 0.0433 mL | 0.2166 mL | 0.4332 mL | 1.0831 mL | |
| 100 mM | 0.0347 mL | 0.1733 mL | 0.3466 mL | 0.8665 mL |
BCDA Related Classifications
HY-52112 Related Classifications
Powered by Bioz