2. Others Metabolic Enzyme/Protease
  3. Environmental Pollutants Endogenous Metabolite
  4. 3-Indoleacetic acid

3-Indoleacetic acid  (Synonyms: Indole-3-acetic acid; 3-IAA)

Cat. No.: HY-18569 Purity: 99.94%
COA
SDS
Handling Instructions Technical Support

3-Indoleacetic acid is is an IAA hormone and growth regulator that can promote plant nutritional growth through processes such as cell expansion, differentiation, morphogenesis, and organogenesis.

For research use only. We do not sell to patients.

CAS No. : 87-51-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
5 g In-stock
10 g In-stock
25 g In-stock
50 g In-stock
100 g In-stock
> 100 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

3-Indoleacetic acid Related Antibodies

Top Publications Citing Use of Products

    3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688.  [Abstract]

    3-IAA (50, 100 mg/kg; once daily; 18 days) significantly inhibited tumor growth in a dose-dependent manner.

    3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688.  [Abstract]

    3-IAA (50, 100 mg/kg; once daily; 6 weeks) significantly reduced bioluminescent signal in the footpads and popliteal lymph nodes; the higher the concentration, the more significant the reduction.

    3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688.  [Abstract]

    The proliferation of T24 and 5637 cells after treatment with different concentrations of 3-IAA for 24 or 48 hours was evaluated using the CCK-8 assay.

    3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688.  [Abstract]

    Representative images of T24 and 5637 cells were observed after treatment with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.

    3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688.  [Abstract]

    Representative images of wound healing assays were observed after treating T24 and 5637 cells with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    3-Indoleacetic acid is is an IAA hormone and growth regulator that can promote plant nutritional growth through processes such as cell expansion, differentiation, morphogenesis, and organogenesis[2].
    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    3-Indoleacetic acid promotes the enrichment of salt tolerant bacteria with growth promoting function during the secondary fermentation process of compost[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    3-Indoleacetic acid Related Antibodies
    In Vivo

    3-Indoleacetic acid (50 g/ha) improves outcrossing rate of diverse rice cytoplasmic male sterile lines and hybrid rice seed production through affecting floral traits[2].
    3-Indoleacetic acid (10 mg/kg) improves photosynthetic performance and reduces the toxic effect of cadmium on C. camphora[3].
    3-Indoleacetic acid (500 mg/kg, once daily, 14 days, p.o.) exhibits adverse effects on liver and kidney function, myocardial and skeletal muscles, and testes in rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: adult male Wistar albino rat[4].
    Dosage: 500 mg/kg body weight, once daily, 14 days
    Administration: oral gavage (p.o.)
    Result: Reduced the number of red blood cells and white blood cells[4].
    Molecular Weight

    175.19

    Formula

    C10H9NO2
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light brown

    SMILES

    OC(CC1=CNC2=C1C=CC=C2)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (713.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1 mg/mL (5.71 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7081 mL 28.5404 mL 57.0809 mL
    5 mM 1.1416 mL 5.7081 mL 11.4162 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  Corn Oil

      Solubility: 6.67 mg/mL (38.07 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 50 mg/mL (285.40 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    SDS (393 KB)

    COA (252 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.7081 mL 28.5404 mL 57.0809 mL 142.7022 mL
    5 mM 1.1416 mL 5.7081 mL 11.4162 mL 28.5404 mL
    DMSO 10 mM 0.5708 mL 2.8540 mL 5.7081 mL 14.2702 mL
    15 mM 0.3805 mL 1.9027 mL 3.8054 mL 9.5135 mL
    20 mM 0.2854 mL 1.4270 mL 2.8540 mL 7.1351 mL
    25 mM 0.2283 mL 1.1416 mL 2.2832 mL 5.7081 mL
    30 mM 0.1903 mL 0.9513 mL 1.9027 mL 4.7567 mL
    40 mM 0.1427 mL 0.7135 mL 1.4270 mL 3.5676 mL
    50 mM 0.1142 mL 0.5708 mL 1.1416 mL 2.8540 mL
    60 mM 0.0951 mL 0.4757 mL 0.9513 mL 2.3784 mL
    80 mM 0.0714 mL 0.3568 mL 0.7135 mL 1.7838 mL
    100 mM 0.0571 mL 0.2854 mL 0.5708 mL 1.4270 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    3-Indoleacetic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    3-Indoleacetic acid87-51-4Indole-3-acetic acid 3-IAAEnvironmental PollutantsEndogenous MetaboliteEnvironmental Contaminantsnatural plant hormonesplant growth regulatorphotosynthesisInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    3-Indoleacetic acid
    Cat. No.:
    HY-18569
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.