3-Indoleacetic acid
Based on 22 publication(s) in Google Scholar
3-Indoleacetic acid is is an IAA hormone and growth regulator that can promote plant nutritional growth through processes such as cell expansion, differentiation, morphogenesis, and organogenesis.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 87-51-4
- Formula: C10H9NO2
- Molecular Weight:175.19
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 3-Indoleacetic acid
More- Cancer Discov. 2025 Jun 11. [Abstract]
- Autophagy. 2025 Oct;21(10):2168-2191. [Abstract]
- Adv Sci (Weinh). 2025 Dec 12:e19735. [Abstract]
- Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
- Exp Mol Med. 2026 Mar;58(3):848-863. [Abstract]
- Plant Commun. 2026 Mar 16:101823. [Abstract]
- Clin Cancer Res. 2026 Apr 21:10.1158/1078-0432.CCR-25-4712. [Abstract]
- Mater Today Bio. 2025 May 6:32:101845. [Abstract]
- Proc Natl Acad Sci U S A. 2023 May 16;120(20):e2303479120. [Abstract]
- Free Radic Biol Med. 2025 Nov 23:243:351-366. [Abstract]
- Food Res Int. 2026 Feb 17.
- Diabetes. 2024 Jun 1;73(6):926-940. [Abstract]
- Cell Rep. 2025 Dec 10;44(12):116673. [Abstract]
- Cell Rep. 2025 Aug 12;44(8):116132. [Abstract]
- Cell Prolif. 2025 Jun;58(6):e13816. [Abstract]
- Plants. 2026 Feb 10;15(4):555. [Abstract]
- Sci Rep. 2025 Jul 7;15(1):24261. [Abstract]
- Mol Med Rep. 2023 Sep;28(3):159. [Abstract]
- Urolithiasis. 2025 Jul 2;53(1):134. [Abstract]
- bioRxiv. 2026 Mar 20.
- ChemRxiv. 2025 Apr 11.
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Imaging
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Cell Imaging/Staining
All Endogenous Metabolite Isoforms
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Biological Activity
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Microbial Metabolite |
Human Endogenous Metabolite |
3-Indoleacetic acid promotes the enrichment of salt tolerant bacteria with growth promoting function during the secondary fermentation process of compost[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:adult male Wistar albino rat[4].
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Dosage:500 mg/kg body weight, once daily, 14 days
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Administration:oral gavage (p.o.)
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Result:Reduced the number of red blood cells and white blood cells[4].
Chemical Information
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CAS No. 87-51-4
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Appearance Solid
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Molecular Weight 175.19
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Formula C10H9NO2
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Color Off-white to light brown
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SMILES
OC(CC1=CNC2=C1C=CC=C2)=O
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Synonyms
Indole-3-acetic acid; 3-IAA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (22)
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Journal Impact Factor
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Most Recent
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Cancer Discov
Gut microbiota modulation through Akkermansia spp. supplementation increases CAR-T cell potency. [Abstract]2025 Jun 11. PMID: 40498998 -
Autophagy
METTL3-dependent m6A modification of SNAP29 induces "autophagy-mitochondrial crisis" in the ischemic microenvironment after soft tissue transplantation. [Abstract]2025 Oct;21(10):2168-2191. PMID: 40340690 -
Adv Sci (Weinh)
Colorectal Cancer Cell's Weapon: RNF32 Engages SPP1+ Macrophages to Foster Liver Metastasis, Targeted by Indole-3-Acetic Acid. [Abstract]2025 Dec 12:e19735. PMID: 41387204 -
Adv Sci (Weinh)
Gut Parabacteroides distasonis-derived Indole-3-Acetic Acid Promotes Phospholipid Remodeling and Enhances Ferroptosis Sensitivity via the AhR-FASN Axis in Bladder Cancer. [Abstract]2025 Jun 25:e04688. PMID: 40557796
3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
3-IAA (50, 100 mg/kg; once daily; 18 days) significantly inhibited tumor growth in a dose-dependent manner.
3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
3-IAA (50, 100 mg/kg; once daily; 6 weeks) significantly reduced bioluminescent signal in the footpads and popliteal lymph nodes; the higher the concentration, the more significant the reduction.
3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
The proliferation of T24 and 5637 cells after treatment with different concentrations of 3-IAA for 24 or 48 hours was evaluated using the CCK-8 assay.
3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
Representative images of T24 and 5637 cells were observed after treatment with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.
3-Indoleacetic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 25:e04688. [Abstract]
Representative images of wound healing assays were observed after treating T24 and 5637 cells with different concentrations of 3-IAA (300 µM and 600 µM; 24 h) or DMSO.
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Exp Mol Med
Proline/serine-rich coiled-coil 1 alleviates atherosclerosis via remodeling tryptophan metabolism mediated by Akkermansia muciniphila. [Abstract]2026 Mar;58(3):848-863. PMID: 41792233 -
Plant Commun
Brassinosteroid signaling regulates PIN5/10-mediated intracellular auxin homeostasis to promote lateral root development in tomato. [Abstract]2026 Mar 16:101823. PMID: 41846312 -
Clin Cancer Res
Dual Inhibition of TRIP13 and Aurora A Induces Mitotic DNA Damage and Concurrent Pyroptotic-apoptotic Cell Death in Rb-deficient Cancer Cells. [Abstract]2026 Apr 21:10.1158/1078-0432.CCR-25-4712. PMID: 42013305 -
Mater Today Bio
Inhaled PAMAM-based nano-formulation prolonged lung retention for alleviating pulmonary inflammation of COPD. [Abstract]2025 May 6:32:101845. PMID: 40469693 -
Proc Natl Acad Sci U S A
The RPA-RNF20-SNF2H cascade promotes proper chromosome segregation and homologous recombination repair. [Abstract]2023 May 16;120(20):e2303479120. PMID: 37155876 -
Free Radic Biol Med
Gut microbial metabolite alleviates osteoporosis by attenuating AKT-NFATc1 signaling pathway and ROS production. [Abstract]2025 Nov 23:243:351-366. PMID: 41290102 -
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Diabetes
Gut microbiota-tryptophan metabolism-GLP-1 axis participates in β-cell regeneration induced by dapagliflozin. [Abstract]2024 Jun 1;73(6):926-940. PMID: 38471012 -
Cell Rep
Aryl hydrocarbon receptor-induced activation of AIM2 inflammasome is mediated by MOMP and MPT: A vital therapeutic pathway for inflammation. [Abstract]2025 Dec 10;44(12):116673. PMID: 41385364 -
Cell Rep
Bifidobacterium animalis suppresses non-small cell lung cancer progression and modulates tumor immunity through indole-3-acetic acid. [Abstract]2025 Aug 12;44(8):116132. PMID: 40802513 -
Cell Prolif
Tryptophan Metabolic Enzyme IL4I1 Inhibits Ferroptosis by Decreasing Ubiquitination of Nrf2 via I3P in Glioblastoma. [Abstract]2025 Jun;58(6):e13816. PMID: 40071723 -
Plants
Integrated Multi-Omics Analysis Revealed the Synergistic Regulatory Mechanisms of Salt Tolerance in Soybean (Kefeng 1). [Abstract]2026 Feb 10;15(4):555. PMID: 41754262 -
Sci Rep
Multi-omics study reveals gut microbiota dysbiosis and tryptophan metabolism alterations in GH-PitNET progression. [Abstract]2025 Jul 7;15(1):24261. PMID: 40624207 -
Mol Med Rep
Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium. [Abstract]2023 Sep;28(3):159. PMID: 37417356 -
Urolithiasis
Gut microbiota-derived indole-3-acetic acid ameliorates calcium oxalate renal stone formation via AHR/NF‑κB axis. [Abstract]2025 Jul 2;53(1):134. PMID: 40601009 -
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Solvent & Solubility
DMSO : 125 mg/mL (713.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1 mg/mL (5.71 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Li Y, et al. Inoculating indoleacetic acid bacteria promotes the enrichment of halotolerant bacteria during secondary fermentation of composting. J Environ Manage. 2022 Nov 15;322:116021. [Content Brief]
[2]. ElShamey EAZ, et al. Growth Regulators Improve Outcrossing Rate of Diverse Rice Cytoplasmic Male Sterile Lines through Affecting Floral Traits. Plants (Basel). 2022 May 12;11(10):1291. [Content Brief]
[3]. Zhou J, et al. Effects of exogenous 3-indoleacetic acid and cadmium stress on the physiological and biochemical characteristics of Cinnamomum camphora. Ecotoxicol Environ Saf. 2020 Mar 15;191:109998. [Content Brief]
[4]. Ismail HTH. Assessment toxic effects of exposure to 3-indoleacetic acid via hemato-biochemical, hormonal, and histopathological screening in rats. Environ Sci Pollut Res Int. 2022 Dec;29(60):90703-90718. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.7081 mL | 28.5404 mL | 57.0809 mL | 142.7022 mL |
| 5 mM | 1.1416 mL | 5.7081 mL | 11.4162 mL | 28.5404 mL | |
| DMSO | 10 mM | 0.5708 mL | 2.8540 mL | 5.7081 mL | 14.2702 mL |
| 15 mM | 0.3805 mL | 1.9027 mL | 3.8054 mL | 9.5135 mL | |
| 20 mM | 0.2854 mL | 1.4270 mL | 2.8540 mL | 7.1351 mL | |
| 25 mM | 0.2283 mL | 1.1416 mL | 2.2832 mL | 5.7081 mL | |
| 30 mM | 0.1903 mL | 0.9513 mL | 1.9027 mL | 4.7567 mL | |
| 40 mM | 0.1427 mL | 0.7135 mL | 1.4270 mL | 3.5676 mL | |
| 50 mM | 0.1142 mL | 0.5708 mL | 1.1416 mL | 2.8540 mL | |
| 60 mM | 0.0951 mL | 0.4757 mL | 0.9513 mL | 2.3784 mL | |
| 80 mM | 0.0714 mL | 0.3568 mL | 0.7135 mL | 1.7838 mL | |
| 100 mM | 0.0571 mL | 0.2854 mL | 0.5708 mL | 1.4270 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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