Indole-3-acetamide

Name: Indole-3-acetamide
Brand: MedChemExpress
SKU: HY-W016784
Rating: 5.0 (2 reviews)

Cat. No.: HY-W016784 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Indole-3-acetamide is a biosynthesis intermediate of 3-Indoleacetic acid (HY-18569). Indole-3-acetic acid is the most common natural plant growth hormone of the auxin class.

For research use only. We do not sell to patients.

CAS No. : 879-37-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Indole-3-acetamide:

Indole-3-acetamide-d₅ In-stock
Indole-3-acetamide (Standard) In-stock

2 Publications Citing Use of MCE Indole-3-acetamide

ELISA

: Indole-3-acetamide purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Aug 11;11(18):8797-8812.; N2a cells were treated with DMSO, Diosmin (Dio; 40 μM), FICZ (250 nM), Indole-3-acetamide (I3C; 10 μM) or L-KN (3 μM) for 12 h. Whole cell extracts were examined by western blotting with the indicated antibodies. NEP expression was adjusted to that of β-actin, which was used as the loading control. n = 3.

: Indole-3-acetamide purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Aug 11;11(18):8797-8812.; N2a-APP cells were treated with Diosmin (Dio; 80 μM), FICZ (250 nM), L-KN (3 μM) or Indole-3-acetamide (I3C; 10 μM) with or without SR1 (2 μM) or Sacubitril (Sac; 1 μM) for 24 h, the supernatant was collected and the Aβ42 levels were determined by ELISA.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Indole-3-acetamide is a biosynthesis intermediate of 3-Indoleacetic acid (HY-18569). Indole-3-acetic acid is the most common natural plant growth hormone of the auxin class^[1].

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Indole-3-acetamide regulates the production of indole-3-acetic acid, which would be affected by factors such as bacterial growth stage, gene expression, and metal ions. Indole-3-acetic acid is a plant growth hormone that regulates the plants physiological processes, such as cell elongation, differentiation, flowering and fruit development^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

174.20

Formula

C₁₀H₁₀N₂O

CAS No.

879-37-8

Appearance

Solid

Color

White to light brown

SMILES

NC(CC1=CNC2=C1C=CC=C2)=O

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (574.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.3 mg/mL (7.46 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7405 mL	28.7026 mL	57.4053 mL
5 mM	1.1481 mL	5.7405 mL	11.4811 mL
10 mM	0.5741 mL	2.8703 mL	5.7405 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2.5 mg/mL (14.35 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (252 KB) HNMR (124 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

References

[1]. Thomas Lehmann, et al. Indole-3-acetamide-dependent auxin biosynthesis: a widely distributed way of indole-3-acetic acid production? Eur J Cell Biol. 2010 Dec;89(12):895-905. [Content Brief]

[2]. Dimkpa CO, et al., Production of indole-3-acetic acid via the indole-3-acetamide pathway in the plant-beneficial bacterium Pseudomonas chlororaphis O6 is inhibited by ZnO nanoparticles but enhanced by CuO nanoparticles. Appl Environ Microbiol. 2012 Mar;78(5):1404-10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.7405 mL	28.7026 mL	57.4053 mL	143.5132 mL
H₂O / DMSO	5 mM	1.1481 mL	5.7405 mL	11.4811 mL	28.7026 mL
DMSO	10 mM	0.5741 mL	2.8703 mL	5.7405 mL	14.3513 mL
	15 mM	0.3827 mL	1.9135 mL	3.8270 mL	9.5675 mL
	20 mM	0.2870 mL	1.4351 mL	2.8703 mL	7.1757 mL
	25 mM	0.2296 mL	1.1481 mL	2.2962 mL	5.7405 mL
	30 mM	0.1914 mL	0.9568 mL	1.9135 mL	4.7838 mL
	40 mM	0.1435 mL	0.7176 mL	1.4351 mL	3.5878 mL
	50 mM	0.1148 mL	0.5741 mL	1.1481 mL	2.8703 mL
	60 mM	0.0957 mL	0.4784 mL	0.9568 mL	2.3919 mL
	80 mM	0.0718 mL	0.3588 mL	0.7176 mL	1.7939 mL
	100 mM	0.0574 mL	0.2870 mL	0.5741 mL	1.4351 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.