5-Bromoindole
Based on 1 Customer Validation
5-Bromoindole is an antibacterial agent with a MIC of 200 μg/mL. 5-Bromoindole inhibits biofilm and curli formation, reduces swarming motility and swimming motility. 5-Bromoindole can be used for the research of enterohemorrhagic escherichia coli o157:h7 infection.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 10075-50-0
- Formula: C8H6BrN
- Molecular Weight:196.05
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
5-bromoindole (50-200 μg/mL; 24 h) inhibits planktonic growth of Enterohemorrhagic Escherichia coli O157:H7[1].
5-bromoindole (2-100 μg/mL; 24 h) dose-dependently inhibits biofilm formation by Enterohemorrhagic Escherichia coli O157:H7, and complete inhibition at 50 and 100 μg/mL[1].
5-bromoindole (400 μg/mL; 2 h) exhibits bactericidal activity against Enterohemorrhagic Escherichia coli O157:H7[1].
5-bromoindole (5-50 μg/mL; 24 h) reduces the number of Enterohemorrhagic Escherichia coli O157:H7 cells in biofilms and eliminates curli fibers, without altering bacterial cell morphology[1].
5-bromoindole (20-50 μg/mL; 24 h) significantly and dose-dependently reduces swarming motility and swimming motility of Enterohemorrhagic Escherichia coli O157:H7[1].
5-bromoindole (20-50 μg/mL; 72 h) inhibits curli production by Enterohemorrhagic Escherichia coli O157:H7, as shown by reduced Congo red staining after 72 h of incubation on agar plates or 24 h of incubation in liquid culture[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10075-50-0
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Appearance Solid
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Molecular Weight 196.05
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Formula C8H6BrN
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Color White to light yellow
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SMILES
BrC1=CC2=C(C=C1)NC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (510.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.1007 mL | 25.5037 mL | 51.0074 mL | 127.5185 mL |
| 5 mM | 1.0201 mL | 5.1007 mL | 10.2015 mL | 25.5037 mL | |
| 10 mM | 0.5101 mL | 2.5504 mL | 5.1007 mL | 12.7518 mL | |
| 15 mM | 0.3400 mL | 1.7002 mL | 3.4005 mL | 8.5012 mL | |
| 20 mM | 0.2550 mL | 1.2752 mL | 2.5504 mL | 6.3759 mL | |
| 25 mM | 0.2040 mL | 1.0201 mL | 2.0403 mL | 5.1007 mL | |
| 30 mM | 0.1700 mL | 0.8501 mL | 1.7002 mL | 4.2506 mL | |
| 40 mM | 0.1275 mL | 0.6376 mL | 1.2752 mL | 3.1880 mL | |
| 50 mM | 0.1020 mL | 0.5101 mL | 1.0201 mL | 2.5504 mL | |
| 60 mM | 0.0850 mL | 0.4251 mL | 0.8501 mL | 2.1253 mL | |
| 80 mM | 0.0638 mL | 0.3188 mL | 0.6376 mL | 1.5940 mL | |
| 100 mM | 0.0510 mL | 0.2550 mL | 0.5101 mL | 1.2752 mL |
5-Bromoindole Related Classifications
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