Dihydrocapsaicin

Name: Dihydrocapsaicin
Brand: MedChemExpress
SKU: HY-N0361
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0361 Pureté: 99.93%: Fiche technique
COA

SDS
Instruction de manipulation
FAQs
Technical Support

Dihydrocapsaicin, a capsaicin, is a potent and selective TRPV1 (transient receptor potential vanilloid channel 1) agonist. Dihydrocapsaicin reduces AIF, Bax, and Caspase-3 expressions, and increased Bcl-2, Bcl-xL and p-Akt levels. Dihydrocapsaicin enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

CAS No. : 19408-84-5

Size	Stock	Quantité

Échantillon gratuit (0.1 - 0.2 mg)	Appliquer maintenant
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	Obtenir un devis
200 mg	Obtenir un devis

or Bulk Inquiry

* Veuillez sélectionner la quantité avant d'ajouter des articles.

This product is a controlled substance and not for sale in your territory.

Avis client

Based on 4 publication(s) in Google Scholar

Other Forms of Dihydrocapsaicin:

Dihydrocapsaicin-d₃ Obtenir un devis
Dihydrocapsaicin (Standard) Obtenir un devis

4 Publications Citing Use of MCE Dihydrocapsaicin

Cell Proliferation/Viability Assay

Apoptosis Analysis

Histological Imaging/Staining

: Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598.; The proliferation of NCI-N87 cells was detected by CCK-8 at 12, 24, and 48 h treated with Dihydrocapsaicin (DHC) (20, 40, 60, 80 μM).

: Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598.; Effect of Dihydrocapsaicin (DHC) (60, 80 μM) on apoptosis of NCI-N87 cells. The apoptosis of NCI-N87 cells was detected by flow cytometry.

: Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Cancer Manag Res. 2026 Mar 21:18:579598.; The apoptosis-related protein expressions of Bax, Caspase3, and Caspase9 in NCI-N87 cells were detected by WB treated with Dihydrocapsaicin (DHC) (60, 80 μM).

: Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]; Immunofluorescence staining for CASP3 (red) and CD31 (green) in the skin tissue of control and Dihydrocapsaicin (DHC) (5 mg/kg, p.o) groups.

: Dihydrocapsaicin purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 May;38(5):2539-2559. [Abstract]; Typical HE staining of skin tissues in control and Dihydrocapsaicin (DHC) (5 mg/kg,p.o.) groups.

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Akt:

Voir toutes les isoformes

Akt Akt1 Akt2 Akt3

Voir tous les produits spécifiques à Isoform TRP Channel:

Voir toutes les isoformes

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Voir tous les produits spécifiques à Isoform Bcl-2 Family:

Voir toutes les isoformes

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

Voir tous les produits spécifiques à Isoform Caspase:

Voir toutes les isoformes

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Voir tous les produits spécifiques à Isoform PI3K:

Voir toutes les isoformes

PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

Activité biologique
Pureté et documentation
Références
Avis client

Description

IC₅₀ & Target

TRPV1

PI3K

Caspase 3

Bax

Bcl-2

Bcl-xL

Cellular Effect

Cell Line	Type	Value	Description	References
Astrocyte	EC₅₀	41.83 μM Compound: 15	Antiproliferative activity against mouse astrocyte cells by MTT assay Antiproliferative activity against mouse astrocyte cells by MTT assay	[PMID: 17417631]
HeLa	IC₅₀	> 50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 29685681]
K562	IC₅₀	> 50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 29685681]
MCF7	IC₅₀	> 50 μM Compound: Dihydrocapsaicin	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 29685681]
Medulloblastoma cell	EC₅₀	0.02 μM Compound: 15	Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay	[PMID: 17417631]

In Vitro

Dihydrocapsaicin (0-100 μM) inhibits platelet aggregation and the activity of clotting factors VIII:C (6.26-100 μM) and IX (25-100 μM)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dihydrocapsaicin (0.5 mg/kg, IP, once) exhibits hypothermic effect and neuroprotection in rat MCAO models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley rats (adult, male, 300-340 g, subjected to right middle cerebral artery occlusion (MCAO)^[2]
Dosage:	0.5 mg/kg
Administration:	IP, once
Result:	Exhibits hypothermic effect, rectal temperature dropped to approximately 35.0 ℃ at 30 min, stayed at equal or below 35.0 ℃ for approximately 20 min, and then gradually returned to approximately 36.5 ℃ at 120 min. Significantly reduced Ischemia-reperfusion induced infarct volume (36.2% ± 2.5%). Reduces ROS levels at 24 h, and reduced ischemia-reperfusion induced a high level of cell death.

Masse moléculaire

307.43

Formule

C₁₈H₂₉NO₃

CAS No.

19408-84-5

Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCCCCCC(NCC1=CC=C(O)C(OC)=C1)=O

Structure Classification

Initial Source

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (325.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2528 mL	16.2639 mL	32.5277 mL
5 mM	0.6506 mL	3.2528 mL	6.5055 mL
10 mM	0.3253 mL	1.6264 mL	3.2528 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.93%

Select Batch:

Fiche technique (272 KB) SDS (480 KB)

COA (242 KB) HNMR (259 KB) LCMS (87 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Gao F, et al. Impairment in function and expression of transient receptor potential vanilloid type 4 in Dahl salt-sensitive rats: significance and mechanism. Hypertension. 2010 Apr;55(4):1018-25. [Content Brief]

[2]. Dihydrocapsaicin, et al. Dihydrocapsaicin (DHC) enhances the hypothermia-induced neuroprotection following ischemic stroke via PI3K/Akt regulation in rat. Brain Res. 2017 Sep 15;1671:18-25. [Content Brief]

[3]. Adams MJ, et al. Effect of capsaicin and dihydrocapsaicin on in vitro blood coagulation and platelet aggregation. Thromb Res. 2009 Dec;124(6):721-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2528 mL	16.2639 mL	32.5277 mL	81.3193 mL
	5 mM	0.6506 mL	3.2528 mL	6.5055 mL	16.2639 mL
	10 mM	0.3253 mL	1.6264 mL	3.2528 mL	8.1319 mL
	15 mM	0.2169 mL	1.0843 mL	2.1685 mL	5.4213 mL
	20 mM	0.1626 mL	0.8132 mL	1.6264 mL	4.0660 mL
	25 mM	0.1301 mL	0.6506 mL	1.3011 mL	3.2528 mL
	30 mM	0.1084 mL	0.5421 mL	1.0843 mL	2.7106 mL
	40 mM	0.0813 mL	0.4066 mL	0.8132 mL	2.0330 mL
	50 mM	0.0651 mL	0.3253 mL	0.6506 mL	1.6264 mL
	60 mM	0.0542 mL	0.2711 mL	0.5421 mL	1.3553 mL
	80 mM	0.0407 mL	0.2033 mL	0.4066 mL	1.0165 mL
	100 mM	0.0325 mL	0.1626 mL	0.3253 mL	0.8132 mL