Cardanol monoene (Synonyms: Cardanol C15:1)

Name: Cardanol monoene
Brand: MedChemExpress
SKU: HY-119979
Rating: 4.5 (0 reviews)

Cat. No.: HY-119979 Purity: 99.51%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Cardanol monoene (Cardanol C15:1) is a phenolic compound which can be found in cashew nut shell liquid. Cardanol monoene can inhibit cancer cells proliferation, migration, cause S phase arrest, induce apoptosis, ROS production and mitochondrial depolarization. Cardanol monoene downregulates MMP-2, MMP-9, cyclinA1 expression, regulates CDK2, p53, Bax, cytochrome c, cleaved caspase-3, cleaved PARP, Apaf-1 expression and Bax/Bcl-2 ratio. Cardanol monoene shows weak DPPH radical scavenging activity and AChE inhibition activity. Cardanol monoene is lethal to Artemia salina nauplii. Cardanol monoene. Cardanol monoene can be used for the research of cancer, infection and inflamation.

For research use only. We do not sell to patients.

Cardanol monoene Chemical Structure

CAS No. : 501-26-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

View All Isoforms

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

View All CDK Isoform Specific Products:

View All Isoforms

CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

View All PARP Isoform Specific Products:

View All Isoforms

PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

View All Cholinesterase (ChE) Isoform Specific Products:

View All Isoforms

AChE BChE

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

MMP-2

MMP-9

cdk2/cyclin A

Caspase 3

Bcl-2

Bax

AChE

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	9.09 6 Compound: 1, cardanol C15:1	Growth inhibition of human A549 cells after 2 days by SRB assay Growth inhibition of human A549 cells after 2 days by SRB assay	[PMID: 9677265]
A549	IC₅₀	9.09 6 Compound: 1, cardanol C15:1	Growth inhibition of human A549 cells after 2 days by SRB assay Growth inhibition of human A549 cells after 2 days by SRB assay	[PMID: 9677265]
A549	IC₅₀	9.09 6 Compound: 1, cardanol C15:1	Growth inhibition of human A549 cells after 2 days by SRB assay Growth inhibition of human A549 cells after 2 days by SRB assay	[PMID: 9677265]
CCD-18Co	IC₅₀	54.58 6 Compound: 1, cardanol C15:1	Growth inhibition of human CCD-18Co cells after 2 days by SRB assay Growth inhibition of human CCD-18Co cells after 2 days by SRB assay	[PMID: 9677265]
CCD-18Co	IC₅₀	54.58 6 Compound: 1, cardanol C15:1	Growth inhibition of human CCD-18Co cells after 2 days by SRB assay Growth inhibition of human CCD-18Co cells after 2 days by SRB assay	[PMID: 9677265]
CCD-18Co	IC₅₀	54.58 6 Compound: 1, cardanol C15:1	Growth inhibition of human CCD-18Co cells after 2 days by SRB assay Growth inhibition of human CCD-18Co cells after 2 days by SRB assay	[PMID: 9677265]
HCT-15	IC₅₀	29.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human HCT15 cells after 2 days by SRB assay Growth inhibition of human HCT15 cells after 2 days by SRB assay	[PMID: 9677265]
HCT-15	IC₅₀	29.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human HCT15 cells after 2 days by SRB assay Growth inhibition of human HCT15 cells after 2 days by SRB assay	[PMID: 9677265]
HCT-15	IC₅₀	29.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human HCT15 cells after 2 days by SRB assay Growth inhibition of human HCT15 cells after 2 days by SRB assay	[PMID: 9677265]
HT1197	IC₅₀	18.81 6 Compound: 1, cardanol C15:1	Growth inhibition of human HT1197 cells after 2 days by SRB assay Growth inhibition of human HT1197 cells after 2 days by SRB assay	[PMID: 9677265]
HT1197	IC₅₀	18.81 6 Compound: 1, cardanol C15:1	Growth inhibition of human HT1197 cells after 2 days by SRB assay Growth inhibition of human HT1197 cells after 2 days by SRB assay	[PMID: 9677265]
HT1197	IC₅₀	18.81 6 Compound: 1, cardanol C15:1	Growth inhibition of human HT1197 cells after 2 days by SRB assay Growth inhibition of human HT1197 cells after 2 days by SRB assay	[PMID: 9677265]
MCF7	IC₅₀	5.56 6 Compound: 1, cardanol C15:1	Growth inhibition of human MCF7 cells after 2 days by SRB assay Growth inhibition of human MCF7 cells after 2 days by SRB assay	[PMID: 9677265]
MCF7	IC₅₀	5.56 6 Compound: 1, cardanol C15:1	Growth inhibition of human MCF7 cells after 2 days by SRB assay Growth inhibition of human MCF7 cells after 2 days by SRB assay	[PMID: 9677265]
MCF7	IC₅₀	5.56 6 Compound: 1, cardanol C15:1	Growth inhibition of human MCF7 cells after 2 days by SRB assay Growth inhibition of human MCF7 cells after 2 days by SRB assay	[PMID: 9677265]
SK-OV-3	IC₅₀	12.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human SKOV3 cells after 2 days by SRB assay Growth inhibition of human SKOV3 cells after 2 days by SRB assay	[PMID: 9677265]
SK-OV-3	IC₅₀	12.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human SKOV3 cells after 2 days by SRB assay Growth inhibition of human SKOV3 cells after 2 days by SRB assay	[PMID: 9677265]
SK-OV-3	IC₅₀	12.9 6 Compound: 1, cardanol C15:1	Growth inhibition of human SKOV3 cells after 2 days by SRB assay Growth inhibition of human SKOV3 cells after 2 days by SRB assay	[PMID: 9677265]

In Vitro

Cardanol monoene (2.5-40 μM; 24-48 h) inhibits human M14 melanoma cell proliferation with IC₅₀ values of 23.15 ± 2.42 μM at 24 h and 12.30 ± 1.67 μM at 48 h, acting in a dose-dependent and time-dependent manner^[1].
Cardanol monoene (10-50 μM; 2 weeks) dose-dependently reduces the colony-formation ability of human M14 melanoma cells, with 40 μM treatment lowering efficiency to 5.46% over 7 days^[1].
Cardanol monoene (10-40 μM; 48 h) dose-dependently inhibits human M14 melanoma cell migration over 48 h by down-regulating MMP-2 and MMP-9 protein expression^[1].
Cardanol monoene (10-40 μM; 24 h) induces dose-dependent S phase arrest in human M14 melanoma cells after 24 h treatment, reducing cyclinA1 mRNA expression and increasing CDK2 mRNA expression to inhibit cell proliferation^[1].
Cardanol monoene (10-50 μM; 24-48 h) induces dose-dependent caspase-mediated apoptosis in human M14 melanoma cells via up-regulating pro-apoptotic proteins (p53, Bax, cytochrome c, cleaved caspase-3, cleaved PARP, Apaf-1), increasing the Bax/Bcl-2 ratio, and down-regulating anti-apoptotic Bcl-2, with 40 μM treatment causing 43.46% apoptotic cells after 24 h^[1].
Cardanol monoene (10-40 μM; 24 h) dose-dependently increases intracellular ROS levels in human M14 melanoma cells after 24 h treatment, with 40 μM causing a 65.27% increase relative to control^[1].
Cardanol monoene (10-40 μM; 24 h) dose-dependently induces mitochondrial depolarization in human M14 melanoma cells after 24 h treatment, with 40 μM reducing the red/green JC-1 fluorescence ratio to 50.37% of control^[1].
Cardanol monoene (40 μM; 24 h) alters the expression of 2527 genes in human M14 melanoma cells, with DEGs enriched in cancer-related pathways including Pathways in cancer, PI3K-Akt, MAPK, and p53 signaling pathways^[1].
Cardanol monoene shows weak DPPH radical scavenging and AChE inhibition activity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	human M14 melanoma cells
Concentration:	2.5, 5, 10, 20, 30, 40 μM
Incubation Time:	24 h; 48 h
Result:	Inhibited M14 cell growth in a dose-dependent and time-dependent manner. Reached IC₅₀ values of 23.15 ± 2.42 μM after 24 h and 12.30 ± 1.67 μM after 48 h of treatment.

Cell Migration Assay ^[1]

Cell Line:	human M14 melanoma cells
Concentration:	10, 20, 30, 40 μM
Incubation Time:	48 h
Result:	Dose-dependently reduced M14 cell migration ability, with wound gap size increasing as cardanol monoene concentration increased. Dose-dependently down-regulated the expression levels of MMP-2 and MMP-9 proteins.

Cell Cycle Analysis^[1]

Cell Line:	human M14 melanoma cells
Concentration:	10, 20, 30, 40 μM
Incubation Time:	24 h
Result:	Induced dose-dependent S phase arrest: increased the proportion of cells in S phase from 16.1% (control) to 37.9% (40 μM). Reduced cyclinA1 mRNA expression and increased CDK2 mRNA expression via Real Time qPCR.

Apoptosis Analysis^[1]

Cell Line:	human M14 melanoma cells
Concentration:	10, 20, 30, 40, 50 μM (AO/EB, Hoechst 33258 staining); 10, 20, 30, 40 μM (Annexin V-FITC/PI staining, western blotting); 30 μM (apoptosis inhibition assay with Z-VAD-FMK (HY-16658B))
Incubation Time:	24 h (Annexin V-FITC/PI staining, western blotting, apoptosis inhibition assay); 24 h, 48 h (AO/EB, Hoechst 33258 staining)
Result:	Dose-dependently increased M14 cell apoptosis rate; raised the proportion of early and late apoptotic cells to 43.46% with 40 μM treatment, compared to 1.85% in the control. Reduced cardanol monoene (30 μM)-induced M14 cell apoptosis ratio from 30.56% to 13.17% when combined with 40 μM Z-VAD-FMK. Induced apoptotic morphological changes including nuclear condensation, bright staining, and fragmentation, which were reduced by Z-VAD-FMK treatment. Dose-dependently up-regulated the expression of p53, cytochrome c, cleaved caspase-3, cleaved PARP, and Apaf-1, increased the Bax/Bcl-2 ratio, and down-regulated Bcl-2 expression at 20, 30, 40 μM. Increased cytochrome c release from mitochondria to cytosol in a dose-dependent manner. Elevated mRNA expression levels of Bax, p53, and p21, down-regulated Bcl-2 mRNA expression, and increased the Bax/Bcl-2 ratio dose-dependently via Real Time qPCR.

In Vivo

Cardanol monoene (1-1000 mg/mL; 24 h) is lethal to Artemia salina nauplii with an LC₅₀ of 43,186.00 ± 1991.00 μg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Artemia salina nauplii^[2]
Dosage:	1, 10, 100, 1000 ppm
Administration:	24 h
Result:	Showed an LC50 of 43,186.00 ± 1991.00 μg/mL.

Molecular Weight

302.49

Formula

C₂₁H₃₄O

CAS No.

501-26-8

Appearance

Oil

Color

Colorless to light yellow

SMILES

OC1=CC=CC(CCCCCCC/C=C\CCCCCC)=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (330.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3059 mL	16.5295 mL	33.0589 mL
5 mM	0.6612 mL	3.3059 mL	6.6118 mL
10 mM	0.3306 mL	1.6529 mL	3.3059 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.51%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (238 KB)

Handling Instructions (2659 KB)

References

[1]. Su WC, et al. Mitochondria-Associated Apoptosis in Human Melanoma Cells Induced by Cardanol Monoene from Cashew Nut Shell Liquid. J Agric Food Chem. 2017;65(28):5620-5631. [Content Brief]

[2]. Rodrigues Valério RB, et al. Unsaturation-Driven Modulation of Antioxidant and Acetylcholinesterase Inhibitory Activities of Cardanol Derivatives. Bioengineering (Basel). 2025;12(12):1316. Published 2025 Dec 1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3059 mL	16.5295 mL	33.0589 mL	82.6474 mL
	5 mM	0.6612 mL	3.3059 mL	6.6118 mL	16.5295 mL
	10 mM	0.3306 mL	1.6529 mL	3.3059 mL	8.2647 mL
	15 mM	0.2204 mL	1.1020 mL	2.2039 mL	5.5098 mL
	20 mM	0.1653 mL	0.8265 mL	1.6529 mL	4.1324 mL
	25 mM	0.1322 mL	0.6612 mL	1.3224 mL	3.3059 mL
	30 mM	0.1102 mL	0.5510 mL	1.1020 mL	2.7549 mL
	40 mM	0.0826 mL	0.4132 mL	0.8265 mL	2.0662 mL
	50 mM	0.0661 mL	0.3306 mL	0.6612 mL	1.6529 mL
	60 mM	0.0551 mL	0.2755 mL	0.5510 mL	1.3775 mL
	80 mM	0.0413 mL	0.2066 mL	0.4132 mL	1.0331 mL
	100 mM	0.0331 mL	0.1653 mL	0.3306 mL	0.8265 mL

Cardanol monoene Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Product Name			Requested Quantity *
Applicant Name *			Salutation
Email Address *			Phone Number *
Department			Organization Name *
City			State
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Cardanol monoene (Synonyms: Cardanol C15:1)

Customer Review

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

View All CDK Isoform Specific Products:

View All Caspase Isoform Specific Products:

View All Bcl-2 Family Isoform Specific Products:

View All PARP Isoform Specific Products:

View All Cholinesterase (ChE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cardanol monoene Related Classifications

Keywords:

Your Recently Viewed Products:

Name
Email *

	Sorry, but the email address you supplied was invalid.

Cardanol monoene (Synonyms: Cardanol C15:1)

Customer Review

Cardanol monoene Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

View All CDK Isoform Specific Products:

View All Caspase Isoform Specific Products:

View All Bcl-2 Family Isoform Specific Products:

View All PARP Isoform Specific Products:

View All Cholinesterase (ChE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cardanol monoene Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cardanol monoene Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report