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  3. Isomerases (EC 5)
  4. Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution)

Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution)

Cat. No.: HY-P70011
COA Handling Instructions

Cyclophilin B/PPIB protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the cis-trans isomerization of proline imide peptide bonds and may contribute to protein folding. This important enzymatic role highlights its importance in ensuring appropriate conformational changes in polypeptide chains, which are critical for functional maturation. Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution) is the recombinant human-derived Cyclophilin B/PPIB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution) is 179 a.a., with molecular weight of ~21.0 kDa.

For research use only. We do not sell to patients.

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Description

Cyclophilin B/PPIB protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the cis-trans isomerization of proline imide peptide bonds and may contribute to protein folding. This important enzymatic role highlights its importance in ensuring appropriate conformational changes in polypeptide chains, which are critical for functional maturation. Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution) is the recombinant human-derived Cyclophilin B/PPIB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution) is 179 a.a., with molecular weight of ~21.0 kDa.

Background

Cyclophilin B/PPIB Protein serves as a peptidyl-prolyl cis-trans isomerase (PPIase), actively engaging in the catalysis of cis-trans isomerization of proline imidic peptide bonds in oligopeptides, thus potentially contributing to the facilitation of protein folding. This enzymatic function underscores the protein's role in the dynamic process of promoting proper conformational changes in polypeptide chains, essential for their functional maturation. As a key player in the intricate realm of protein folding, Cyclophilin B/PPIB plays a crucial part in maintaining cellular protein homeostasis by facilitating the correct folding of nascent or misfolded polypeptides. The precise orchestration of these PPIase activities underscores the protein's importance in cellular physiology, warranting further exploration to unveil the specific molecular mechanisms and cellular pathways through which Cyclophilin B/PPIB contributes to protein folding dynamics.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P23284 (D34-A212)

Gene ID
Molecular Construction
N-term
PPIB (D34-A212)
Accession # P23284
6*His
C-term
Synonyms
rHuPeptidyl-prolyl cis-trans isomerase B/PPIB, His; CYP-S1; CYPB; HEL-S-39; OI9; SCYLP
AA Sequence

DEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFA

Molecular Weight

Approximately 21.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 6.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Cyclophilin B/PPIB Protein, Human (HEK293, C-His, solution)
Cat. No.:
HY-P70011
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