Cyclo(Arg-Gly-Asp-D-Phe-Cys)
Based on 2 publication(s) in Google Scholar
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (Cyclo RGDfC), a cyclic RGD peptide which has high affinity to αvβ3, can disrupt cell integrin interactions. Cyclo(Arg-Gly-Asp-D-Phe-Cys) inhibits pluripotent marker expression in embryonic stem cells (ESCs) and the tumorigenic potential of mESCs in vivo. Cyclo(Arg-Gly-Asp-D-Phe-Cys) can be used in the research of tumors.
For research use only. We do not sell to patients.
- Purity: 98.08%
- CAS No.: 862772-11-0
- Formula: C24H34N8O7S
- Molecular Weight:578.64
-
Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cyclo(Arg-Gly-Asp-D-Phe-Cys)
More
Biological Activity
|
αvβ3 |
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM; 24 h) down-regulates the transcription factors Oct 4, Sox 2 and Nanog of mESCs[1].
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM) inhibits integrin gene expression mESC-col I (type I collagen) constructs[1].
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM)-treated mESC leads to the formation of aggregates and detachment from the surface[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MESCs
-
Concentration:0.5 mM
-
Incubation Time:24 h
-
Result:Down-regulated Oct 4, Nanog, Sox 2 by 99%, 97.5%, and 90% respectively.
Up-regulated Rex1 gene expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 862772-11-0
-
Appearance Solid
-
Molecular Weight 578.64
-
Formula C24H34N8O7S
-
Color White to off-white
-
Synonyms
Cyclo(RGDfC)
-
Sequence
Cyclo(Arg-Gly-Asp-D-Phe-Cys)
-
Sequence Shortening
cyclo(RGDfC)
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
J Mol Cell Cardiol
Vinculin haploinsufficiency impairs integrin-mediated costamere remodeling on stiffer microenvironments. [Abstract]2025 Mar:200:1-10. PMID: 39793757 -
Solvent & Solubility
DMSO : 50 mg/mL (86.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7282 mL | 8.6410 mL | 17.2819 mL | 43.2048 mL |
| 5 mM | 0.3456 mL | 1.7282 mL | 3.4564 mL | 8.6410 mL | |
| 10 mM | 0.1728 mL | 0.8641 mL | 1.7282 mL | 4.3205 mL | |
| 15 mM | 0.1152 mL | 0.5761 mL | 1.1521 mL | 2.8803 mL | |
| 20 mM | 0.0864 mL | 0.4320 mL | 0.8641 mL | 2.1602 mL | |
| 25 mM | 0.0691 mL | 0.3456 mL | 0.6913 mL | 1.7282 mL | |
| 30 mM | 0.0576 mL | 0.2880 mL | 0.5761 mL | 1.4402 mL | |
| 40 mM | 0.0432 mL | 0.2160 mL | 0.4320 mL | 1.0801 mL | |
| 50 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL | 0.8641 mL | |
| 60 mM | 0.0288 mL | 0.1440 mL | 0.2880 mL | 0.7201 mL | |
| 80 mM | 0.0216 mL | 0.1080 mL | 0.2160 mL | 0.5401 mL |
Cyclo(Arg-Gly-Asp-D-Phe-Cys) Related Classifications
HY-P2300 Related Classifications
Powered by Bioz