Cinnamosyn
Cinnamosyn is a 10-mer N-cinnamoyl-containing peptide that is cytotoxic to human cells.
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- Formel: C60H90N12O12
- Molecular Weight:1171.43
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Beschreibung
Chemical Information
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Molecular Weight 1171.43
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Formel C60H90N12O12
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Sequence
Cyclo({(E)-Mecinn}-{allo-Thr}-Phe-{d-Leu}-Val-Ala-{d-Orn}-Gly-Pro-Orn-Leu)
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Sequence Shortening
Cyclo({(E)-Mecinn}-{allo-Thr}-F-{d-Leu}-VA-{d-Orn}-GP-{Orn}-L)
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)