Cyclosomatostatin TFA
Based on 1 publication(s) in Google Scholar
Cyclosomatostatin TFA is a non-selective somatostatin receptor antagonist that blocks SSTR1-5-mediated signaling. Cyclosomatostatin TFA decreases the ALDH+ stem cell population and sphere formation without affecting cell viability, and reduces cell proliferation. Cyclosomatostatin TFA activates opioid receptors. Cyclosomatostatin TFA can be used for research on arthritis and colorectal cancer.
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- Formel: C46H58F3N7O8
- Molecular Weight:893.99
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cyclosomatostatin TFA
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Biologische Aktivität
Beschreibung
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SSTR1 |
In Vitro
Cyclosomatostatin (3-10 μM; 5 min) TFA inhibits nerve-mediated cholinergic contractions of the rat stomach fundus strip[4].
Cyclosomatostatin (1 μM; 10 min) TFA prevents SST-mediated hyperpolarization and reduced excitability of mouse prelimbic cortex pyramidal neurons, demonstrating that these effects are dependent on SSTRs[5].
Cyclosomatostatin (1 μM) TFA largely does not reverse SST-mediated hyperpolarization and reduced excitability of mouse prelimbic cortex pyramidal neurons[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT29 and SW480
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Concentration:10 μM
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Incubation Time:48 h
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Result:Decreased ALDH+ cells, cell count by 50%, and sphere formation in HT29 cells without affecting cell viability.
Decreased ALDH+ cells, sphere formation, and cell count by 30% in SW480 cells without affecting cell viability.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (adult male, 240-420 g)[1]
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Dosage:0.1 mM, 0.2 mL
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Administration:i.a.; single injection
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Result:Increased responses to noxious movements in half of the units, with a mean response of approximately 150% of control values.
The effect was transient, returning to control levels after about 30 minutes.
- Mammalian live/dead viability and cytotoxicity staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Collagen-Induced Arthritis
- Ki-67 Immunostaining Proliferation Assay
- Cell Cytotoxicity Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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Molecular Weight 893.99
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Formel C46H58F3N7O8
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Sequence
cyclo(7-Aminoheptanoyl-Phe-dTrp-Lys-Thr(Bzl))
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Sequence Shortening
Cyclo(7-Aminoheptanoyl-FwK-Thr(Bzl))
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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J Clin Invest
Genomic and transcriptomic features of androgen receptor signaling inhibitor resistance in metastatic castration-resistant prostate cancer. [Abstract]2024 Aug 13;134(19):e178604. PMID: 39352383
Lösungsmittel & Löslichkeit
In Vitro
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Cyclosomatostatin TFA Related Classifications
HY-P1201A Related Classifications
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Keywords
- Cyclosomatostatin
- Somatostatin Receptor
- Opioid Receptor
- articular afferents
- non-selective somatostatin receptor antagonist
- rat hepatocytes
- SSTR1-5-mediated signaling
- rat liver basolateral plasma membranes
- HT29 and SW480 colorectal cancer cell lines
- ALDH+ stem cell population
- pI 6.1 carboxylesterase inhibitor
- rat stomach fundus strip
- guinea-pig ileum
- Inhibitor
- inhibitor
- inhibit