expressed

Oxygen homeostasis regulation is the most fundamental cellular process for adjusting physiological oxygen variations, and its irregularity leads to various human diseases, including cancer. Hypoxia is closely associated with cancer development, and hypoxia/oxygen-sensing signaling plays critical roles in the modulation of cancer progression.

Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that functions as a master regulator of oxygen homeostasis. A variety of HF-1 target genes have been identified thus far which encode proteins that play key roles in critical developmental and physiological processes including angiogenesis/vascular remodeling, erythropoiesis, glucose transport, glycolysis, iron transport, and cell proliferation/survival.

HIF-1 is a heterodimeric transcription factor consisting of a constitutively expressed β-subunit and an oxygen-regulated α-subunit. The unique feature of HIF-1 is the regulation of HIF-1α expression and activity based upon the cellular O₂ concentration. Under normoxic conditions, hydroxylation of HIF-1α on these different proline residues is essential for HIF proteolytic degradation by promoting interaction with the von Hippel-Lindau tumor-suppressor protein (pVHL) through hydrogen bonding to the hydroxyproline-binding pocket in the pVHL β-domain. As oxygen levels decrease, hydroxylation of HIF decreases; HIF-1α then no longer binds pVHL, and becomes stabilized, allowing more of the protein to translocate to the cell’s nucleus, where it acts as a transcription factor, upregulating (often within minutes) the production of proteins that stimulate blood perfusion in tissues and thus tissue oxygenation.

MCE offers a unique collection of 2507 oxygen sensing related compounds targeting HIF/HIF Prolyl-Hydroxylase, MAPK/ERK, PI3K/AKT signaling pathways, etc. MCE Oxygen Sensing Compound Library is a useful tool to study hypoxia, oxidative stress and discover new anti-cancer drugs.

RNase Inhibitor is a 50 kDa recombinant protein expressed in E.coli, and can specifically inhibit RNase A, B and C.

RNase H is an endoribonuclease expressed and purified from Escherichia coli and can specifically hydrolyze RNA in DNA-RNA hybrid strands.

This product is obtained by expressing the Exo I gene (E. coli) in Escherichia coli and then purifying and isolating it multiple times.

RNase III is a specific exonuclease expressed in E.coli that can cleave dsRNA into 12-15 bp dsRNA fragments.

Reverse Transcriptase is a reverse transcriptase that clones and expresses the deletion mutant RNase H- of M-MuLV through genetic recombination technology.

This product is a DNA polymerase obtained by expressing DNA Polymerase I (E. coli) in E. coli and purifying and isolating it multiple times.

MCE Anti-His Magnetic Beads are used for immunoprecipitation (IP) of specific His-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

This enzyme is a DNA ligase cloned from Thermus aquaticus HB8. It is expressed in E. coli using genetic recombination technology and purified and isolated multiple times.

MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-HA Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and their fusion-expressed proteins. Moreover, it can be utilized for immunoprecipitation (IP) assays.

MCE Anti-c-Myc Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

Bst DNA Polymerase large fragment is a part of Bacillus stearothermophilus DNA polymerase, which is derived from E. coli strain. It is expressed in E. coli and purified and isolated multiple times.

MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Anti-HA Affinity Gel can be employed for the detection and purification of HA fusion expressed proteins, and can also be used in immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

MCE Anti-GFP Affinity Gel is produced through the covalent coupling of high-quality GFP antibody with agarose. It boasts a high loading capacity, exceptional specificity, and stability. This gel can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins. Moreover, it can be utilized for cimmunoprecipitation (IP) assays without binding to BFP-tagged proteins.