2. Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation
  3. Apoptosis Arginase
  4. nor-NOHA dihydrochloride

nor-NOHA dihydrochloride  (Synonyms: Nω-Hydroxy-nor-L-arginine dihydrochloride)

Cat. No.: HY-112885C Purity: 99.61%
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nor-NOHA dihydrochloride is a selective and reversible Arginase inhibitor. nor-NOHA dihydrochloride induces Apoptosis in ARG2-expressing cells under hypoxia. nor-NOHA dihydrochloride has anti-leukemic activity. nor-NOHA dihydrochloride improves liver ischemia-reperfusion injury. nor-NOHA dihydrochloride can used in study of tuberculosis, endothelial dysfunction, immunosuppression and metabolism.

For research use only. We do not sell to patients.

CAS No. : 291758-32-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of nor-NOHA dihydrochloride:

nor-NOHA dihydrochloride Related Antibodies

Top Publications Citing Use of Products

    nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7291-7308.e23.  [Abstract]

    Bar plots showing proportions of TNF-α+ T cells in fetal liver whole blood following PMA simulation. T cells were cultured alone (light green) or co-cultured with matched fetal neutrophils (orange) in the presence or absence of ARG1 inhibitor (nonor-NOHA monoacetate; overnight) or L-arginine supplementation, as indicated on the x axis.

    nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Sep 30;39(18):e71057.  [Abstract]

    nonor-NOHA monoacetate (1, 10, 50, 100 μM) increased HaCaT cell viability.

    nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Sep 30;39(18):e71057.  [Abstract]

    nonor-NOHA monoacetate (100 μM) increased K5 expression in HaCaT cells.

    nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Dec 23;9:741911.  [Abstract]

    nor-NOHA monoacetate (nor-NOHA) efficiently reduced immunosuppressive activity. Arg1 inhibitor (nor-NOHA) and/or iNOS inhibitor [L-NG-Monomethylarginine, Acetate Salt (L-NMMA)] were added in PMN-MDSCs’ suppressive function assays.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    nor-NOHA dihydrochloride is a selective and reversible Arginase inhibitor. nor-NOHA dihydrochloride induces Apoptosis in ARG2-expressing cells under hypoxia. nor-NOHA dihydrochloride has anti-leukemic activity. nor-NOHA dihydrochloride improves liver ischemia-reperfusion injury. nor-NOHA dihydrochloride can used in study of tuberculosis, endothelial dysfunction, immunosuppression and metabolism[1][2][3][4].

    IC50 & Target

    Arginase[1]
    In Vitro

    nor-NOHA (0.1-1 mM, 72 h) dihydrochloride induces apoptosis in a dose-dependent manner under hypoxia (1.5% O2) in K562 cells[1].
    nor-NOHA (1 mM, 72 h) dihydrochloride attenuates hypoxia-mediated resistance towards Imatinib (HY-15463) in K562 or KCL22 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    nor-NOHA dihydrochloride Related Antibodies
    In Vivo

    Nor-NOHA (0.04 mg/g of mouse; i.p.; daily) dihydrochloride modulates arginase to alter immune responses in experimental mouse models of pulmonary tuberculosis, including in the setting of HIV co-infection[4].
    nor-NOHA (100 mg/kg, i.v., once time) dihydrochloride induces a significant reduction of infarct size in male Sprague-Dawley rats[2].
    nor-NOHA (100 mg/kg, i.v., once time) dihydrochloride increases plasma citrulline and nitrite levels and decreases the ornithine plasma levels in male Sprague-Dawley rats[2].
    nor-NOHA (100 mg/kg; intravenous penile vein injection) dihydrochloride improves liver I/R injury[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Sprague-Dawley rats subjected to ischemia-reperfusion[2]
    Dosage: 100 mg/kg
    Administration: Intravenous injection (i.v.)
    Result: Induced a significant reduction of infarct size to 39%.
    Did not reduce infarct size when administered together with cPTIO or L-NMMA.
    Molecular Weight

    249.10

    Formula

    C5H14Cl2N4O3
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    OC([C@@H](N)CCNC(NO)=N)=O.Cl.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (401.45 mM)

    DMSO : 100 mg/mL (401.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.0145 mL 20.0723 mL 40.1445 mL
    5 mM 0.8029 mL 4.0145 mL 8.0289 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.61%

    SDS (252 KB)

    COA (246 KB) HNMR (448 KB) RP-HPLC (206 KB) LCMS (88 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.0145 mL 20.0723 mL 40.1445 mL 100.3613 mL
    5 mM 0.8029 mL 4.0145 mL 8.0289 mL 20.0723 mL
    10 mM 0.4014 mL 2.0072 mL 4.0145 mL 10.0361 mL
    15 mM 0.2676 mL 1.3382 mL 2.6763 mL 6.6908 mL
    20 mM 0.2007 mL 1.0036 mL 2.0072 mL 5.0181 mL
    25 mM 0.1606 mL 0.8029 mL 1.6058 mL 4.0145 mL
    30 mM 0.1338 mL 0.6691 mL 1.3382 mL 3.3454 mL
    40 mM 0.1004 mL 0.5018 mL 1.0036 mL 2.5090 mL
    50 mM 0.0803 mL 0.4014 mL 0.8029 mL 2.0072 mL
    60 mM 0.0669 mL 0.3345 mL 0.6691 mL 1.6727 mL
    80 mM 0.0502 mL 0.2509 mL 0.5018 mL 1.2545 mL
    100 mM 0.0401 mL 0.2007 mL 0.4014 mL 1.0036 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    nor-NOHA dihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    nor-NOHA291758-32-2Nω-Hydroxy-nor-L-arginineApoptosisArginaseArginase InhibitorARG2Anti-leukemicImunosuppressionEdothelial dysfunctionInhibitorinhibitorinhibit

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