nor-NOHA dihydrochloride
Based on 7 publication(s) in Google Scholar
nor-NOHA dihydrochloride is a selective and reversible Arginase inhibitor. nor-NOHA dihydrochloride induces Apoptosis in ARG2-expressing cells under hypoxia. nor-NOHA dihydrochloride has anti-leukemic activity. nor-NOHA dihydrochloride improves liver ischemia-reperfusion injury. nor-NOHA dihydrochloride can used in study of tuberculosis, endothelial dysfunction, immunosuppression and metabolism.
For research use only. We do not sell to patients.
- Purity: 99.61%
- CAS No.: 291758-32-2
- Formula: C5H14Cl2N4O3
- Molecular Weight:249.10
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
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Publications Citing Use of MedChemExpress (MCE) nor-NOHA dihydrochloride
More- Cell. 2025 Dec 11;188(25):7291-7308.e23. [Abstract]
- Free Radic Biol Med. 2026 Apr:247:516-527. [Abstract]
- Chin Med. 2026 May 28;21(1):151. [Abstract]
- Front Cell Dev Biol. 2021 Dec 23;9:741911. [Abstract]
- FASEB J. 2025 Sep 30;39(18):e71057. [Abstract]
- Clin Immunol. 2024 Nov:268:110355. [Abstract]
- Res Sq. 2026 May 8:rs.3.rs-9404453. [Abstract]
Customer Validation & Images
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Flow Cytometry
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Cell Proliferation/Viability Assay
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RT-PCR
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Bio/Physico-chemical Assay
Biological Activity
Arginase[1]
nor-NOHA (0.1-1 mM, 72 h) dihydrochloride induces apoptosis in a dose-dependent manner under hypoxia (1.5% O2) in K562 cells[1].
nor-NOHA (1 mM, 72 h) dihydrochloride attenuates hypoxia-mediated resistance towards Imatinib (HY-15463) in K562 or KCL22 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
nor-NOHA (100 mg/kg, i.v., once time) dihydrochloride induces a significant reduction of infarct size in male Sprague-Dawley rats[2].
nor-NOHA (100 mg/kg, i.v., once time) dihydrochloride increases plasma citrulline and nitrite levels and decreases the ornithine plasma levels in male Sprague-Dawley rats[2].
nor-NOHA (100 mg/kg; intravenous penile vein injection) dihydrochloride improves liver I/R injury[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats subjected to ischemia-reperfusion[2]
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Dosage:100 mg/kg
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Administration:Intravenous injection (i.v.)
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Result:Induced a significant reduction of infarct size to 39%.
Did not reduce infarct size when administered together with cPTIO or L-NMMA.
Chemical Information
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CAS No. 291758-32-2
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Appearance Solid
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Molecular Weight 249.10
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Formula C5H14Cl2N4O3
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Color Off-white to light yellow
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SMILES
OC([C@@H](N)CCNC(NO)=N)=O.Cl.Cl
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Synonyms
Nω-Hydroxy-nor-L-arginine dihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Cell
2025 Dec 11;188(25):7291-7308.e23. PMID: 41161316
nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7291-7308.e23. [Abstract]
Bar plots showing proportions of TNF-α+ T cells in fetal liver whole blood following PMA simulation. T cells were cultured alone (light green) or co-cultured with matched fetal neutrophils (orange) in the presence or absence of ARG1 inhibitor (nonor-NOHA monoacetate; overnight) or L-arginine supplementation, as indicated on the x axis.
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Free Radic Biol Med
Endothelial Arg2 regulates HIMM-induced mitochondrial hyperfission via affecting arginine metabolism. [Abstract]2026 Apr:247:516-527. PMID: 41720362 -
Chin Med
Licoisoflavone A potentiates oxaliplatin efficacy in colorectal cancer by dual targeting arginine metabolism of tumor microenvironment and cancer cells. [Abstract]2026 May 28;21(1):151. PMID: 42210399 -
Front Cell Dev Biol
mTOR Signaling Regulates the Development and Therapeutic Efficacy of PMN-MDSCs in Acute GVHD. [Abstract]2021 Dec 23;9:741911. PMID: 35004668
nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Dec 23;9:741911. [Abstract]
nor-NOHA monoacetate (nor-NOHA) efficiently reduced immunosuppressive activity. Arg1 inhibitor (nor-NOHA) and/or iNOS inhibitor [L-NG-Monomethylarginine, Acetate Salt (L-NMMA)] were added in PMN-MDSCs’ suppressive function assays.
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FASEB J
Spatial Metabolism of Primary Limited Cutaneous Amyloidosis Based on Mass Spectrometry Imaging. [Abstract]2025 Sep 30;39(18):e71057. PMID: 40955599
nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Sep 30;39(18):e71057. [Abstract]
nonor-NOHA monoacetate (1, 10, 50, 100 μM) increased HaCaT cell viability.
nor-NOHA dihydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Sep 30;39(18):e71057. [Abstract]
nonor-NOHA monoacetate (100 μM) increased K5 expression in HaCaT cells.
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Clin Immunol
MAPK signaling pathway induced LOX-1+ polymorphonuclear myeloid-derived suppressor cells in biliary atresia. [Abstract]2024 Nov:268:110355. PMID: 39237078 -
Res Sq
Protective coinfection: influenza reprograms myeloid cells to limit CD8 T cell-mediated malaria pathology. [Abstract]2026 May 8:rs.3.rs-9404453. PMID: 42147182
Solvent & Solubility
H2O : ≥ 100 mg/mL (401.45 mM)
DMSO : 100 mg/mL (401.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ng KP, et al. The arginase inhibitor Nω-hydroxy-nor-arginine (nor-NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. PLoS One. 2018 Oct 11;13(10):e0205254. [Content Brief]
[2]. Jung C, et al. Arginase inhibition mediates cardioprotection during ischaemia-reperfusion. Cardiovasc Res. 2010 Jan 1;85(1):147-54. [Content Brief]
[3]. Reid KM, et al. Liver I/R injury is improved by the arginase inhibitor, N(omega)-hydroxy-nor-L-arginine (nor-NOHA). Am J Physiol Gastrointest Liver Physiol. 2007 Feb;292(2):G512-7. [Content Brief]
[4]. Chauhan , et al. Therapeutic Modulation of Arginase with nor-NOHA Alters Immune Responses in Experimental Mouse Models of Pulmonary Tuberculosis including in the Setting of Human Immunodeficiency Virus (HIV) Co-Infection. Trop Med Infect Dis. 2024 Jun 6;9(6):129. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 4.0145 mL | 20.0723 mL | 40.1445 mL | 100.3613 mL |
| 5 mM | 0.8029 mL | 4.0145 mL | 8.0289 mL | 20.0723 mL | |
| 10 mM | 0.4014 mL | 2.0072 mL | 4.0145 mL | 10.0361 mL | |
| 15 mM | 0.2676 mL | 1.3382 mL | 2.6763 mL | 6.6908 mL | |
| 20 mM | 0.2007 mL | 1.0036 mL | 2.0072 mL | 5.0181 mL | |
| 25 mM | 0.1606 mL | 0.8029 mL | 1.6058 mL | 4.0145 mL | |
| 30 mM | 0.1338 mL | 0.6691 mL | 1.3382 mL | 3.3454 mL | |
| 40 mM | 0.1004 mL | 0.5018 mL | 1.0036 mL | 2.5090 mL | |
| 50 mM | 0.0803 mL | 0.4014 mL | 0.8029 mL | 2.0072 mL | |
| 60 mM | 0.0669 mL | 0.3345 mL | 0.6691 mL | 1.6727 mL | |
| 80 mM | 0.0502 mL | 0.2509 mL | 0.5018 mL | 1.2545 mL | |
| 100 mM | 0.0401 mL | 0.2007 mL | 0.4014 mL | 1.0036 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
nor-NOHA dihydrochloride Related Classifications
HY-112885C Related Classifications
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