1. Immunology/Inflammation
  2. NO Synthase
  3. L-NAME hydrochloride

L-NAME hydrochloride (Synonyms: NG-Nitroarginine methyl ester hydrochloride)

Cat. No.: HY-18729A Purity: 99.07%
Handling Instructions

L-NAME hydrochloride inhibits NOS with an IC50 of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC50 value of 1.4 μM.

For research use only. We do not sell to patients.

L-NAME hydrochloride Chemical Structure

L-NAME hydrochloride Chemical Structure

CAS No. : 51298-62-5

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Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    L-NAME hydrochloride purchased from MCE. Usage Cited in: Bosn J Basic Med Sci. 2017 May 20;17(2):132-137.

    Effects of Akt/adenosine monophosphate-activated protein kinaseon Valsartan(VAL)-mediated endothelial nitric oxide (NO) synthase (eNOS) phosphorylation and NO production in human umbilical vein endothelial cells (HUVECs). (A) The variation of VAL (10 μM)-induced eNOS activation after HUVECs are incubated with LY294002 [LY] (10 μM), Compound C (1 μM), L-NAME (500 μM) for 3 hours. (B)The variation of VAL (10 μM)-induced nitric oxide (NO) productionafter HUVECs are incubated with LY294002 (10 μM),

    L-NAME hydrochloride purchased from MCE. Usage Cited in: Exp Ther Med. 2018 Aug;16(2):1079-1086.

    Effect of L-name on the expression of eNOS, HSP90, Nrf2, Nqo1 and HO-1 in a rat model of spinal cord injury (SCI).
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    Description

    L-NAME hydrochloride inhibits NOS with an IC50 of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC50 value of 1.4 μM.

    IC50 & Target

    IC50: 70 μM (NOS)[1]

    In Vitro

    L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with Nw-nitro-L-arginine methyl ester (L-NAME) being at the head[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC50= 70 μM) than L-NOARG (IC50= 1.4 μM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    L-NAME infusion significantly decreases NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and interferon-gamma production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes[3]. There is increasing evidence that nitric oxide may be involved in learning and memory. l-NAME produces a task-dependent impairment of fear extinction, and implies that nitric oxide signaling is involved in memory process of certain fear extinction tasks[4]. Chronic L-NAME administration induces cardiac hypertrophy in rodent models. Six weeks L-NAME administration induces significant cardiac hypertrophy compared to control hearts[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    269.69

    Formula

    C₇H₁₆ClN₅O₄

    CAS No.

    51298-62-5

    SMILES

    N[[email protected]@H](CCCNC(N[N+]([O-])=O)=N)C(OC)=O.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (463.50 mM; Need ultrasonic)

    DMSO : 100 mg/mL (370.80 mM; Need ultrasonic)

    H2O : ≥ 32 mg/mL (118.65 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7080 mL 18.5398 mL 37.0796 mL
    5 mM 0.7416 mL 3.7080 mL 7.4159 mL
    10 mM 0.3708 mL 1.8540 mL 3.7080 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Animal Administration
    [3][5]

    Rats: The purpose is to investigate dose effects of chronically infused NOS inhibitor, LNAME on the anabolism, inflammatory responses, and arginine metabolism in parenterally fed rats with cecal puncture-induced subacute peritonitis. Male Wistar rats (8-9 weeks old), initially weighing 250 g, are used in the study. Rats are divided into 4 groups and are administered total parenteral nutrition solutions with 0, 5 (low dose), 25 (medium dose), or 50 (high dose) mg/kg per day of L-NAME for 7 days[3].

    Mice: 12-20 week old C57BL/6J mice (5 per group) are administered L-NAME (0.325mg/mL) in the drinking water. Hearts are excised at 1-day, 2-days, 5-days, 2-weeks or 6-weeks; or controls which received no L-NAME. Ventricular cross-sectional wall thickness and individual cardiac myocytes cross-sectional area and cardiomyocyte/nuclear ratio to determine cardiac hypertrophy. Immuno-histochemical staining for c-kit, sca-1 and BCRP undertaken[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.07%

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    Keywords:

    L-NAMENG-Nitroarginine methyl esterNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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    Product Name:
    L-NAME hydrochloride
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