1. Induced Disease Models Products Immunology/Inflammation
  2. Cardiovascular System Disease Models NO Synthase
  3. Hypertension Models
  4. L-NAME hydrochloride

L-NAME hydrochloride  (Synonyms: NG-Nitroarginine methyl ester hydrochloride)

Cat. No.: HY-18729A Purity: 99.70%
COA Handling Instructions

L-NAME hydrochloride inhibits NOS with an IC50 of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC50 value of 1.4 μM.

For research use only. We do not sell to patients.

L-NAME hydrochloride Chemical Structure

L-NAME hydrochloride Chemical Structure

CAS No. : 51298-62-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in Water USD 61 In-stock
Solid
100 mg USD 25 In-stock
500 mg USD 35 In-stock
1 g USD 50 In-stock
5 g USD 140 In-stock
10 g USD 200 In-stock
50 g   Get quote  

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Customer Review

Based on 38 publication(s) in Google Scholar

Top Publications Citing Use of Products

35 Publications Citing Use of MCE L-NAME hydrochloride

WB
IF

    L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Mar 13;161:114484.  [Abstract]

    L-NAME (10 mg/kg; i.p.; single daily for 7 days; pretreat) alleviates Candesartan-induced glomerular shrinkage and renal tubular necrosis and detachment, in low-salt rats.

    L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2018 Aug;16(2):1079-1086.  [Abstract]

    Effect of L-name on the expression of eNOS, HSP90, Nrf2, Nqo1 and HO-1 in a rat model of spinal cord injury (SCI).

    L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Bosn J Basic Med Sci. 2017 May 20;17(2):132-137.  [Abstract]

    Effects of Akt/adenosine monophosphate-activated protein kinaseon CGP 48933 (VAL)-mediated endothelial nitric oxide (NO) synthase (eNOS) phosphorylation and NO production in human umbilical vein endothelial cells (HUVECs). (A) The variation of VAL (10 μM)-induced eNOS activation after HUVECs are incubated with LY294002 [LY] (10 μM), Compound C (1 μM), L-NAME (500 μM) for 3 hours. (B)The variation of VAL (10 μM)-induced nitric oxide (NO) productionafter HUVECs are incubated with LY294002 (10 μM),
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-NAME hydrochloride inhibits NOS with an IC50 of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC50 value of 1.4 μM.

    IC50 & Target

    IC50: 70 μM (NOS)[1]

    In Vitro

    L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with Nw-nitro-L-arginine methyl ester (L-NAME) being at the head[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC50= 70 μM) than L-NOARG (IC50= 1.4 μM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    L-NAME hydrochloride can be used in animal modeling to construct cardiovascular and cerebrovascular disease models.

    L-NAME infusion significantly decreases NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and IFNγ production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes[3]. There is increasing evidence that nitric oxide may be involved in learning and memory. l-NAME produces a task-dependent impairment of fear extinction, and implies that nitric oxide signaling is involved in memory process of certain fear extinction tasks[4]. Chronic L-NAME administration induces cardiac hypertrophy in rodent models. Six weeks L-NAME administration induces significant cardiac hypertrophy compared to control hearts[5].

    b>L-NAME hydrochloride is a classic hypertension modeling agent that decreases nitric oxide (NO) release with an inhibition competence in endothelial nitric oxide synthase (eNOS) in animals. Rats and mice are generally used as animal models[6][7].

    Dose reference for L-NAME hydrochloride induction[6][7]:
    (1) Model animal: Swiss Webster male mice
    Hypertension Model: 400 mg/kg/day, i.p, 7 day
    (2) Model animals: Male Sprague-Dawley (SD) rats
    Hypertension Model: 40 mg/kg, drinking water, 5 weeks

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Sprague-Dawley (SD) rats [7]
    Dosage: 40 mg/kg, 5 weeks
    Administration: drinking water
    Result: Induced hypertension with body weight loss and high blood pressure.
    Clinical Trial
    Molecular Weight

    269.69

    Formula

    C7H16ClN5O4

    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(OC)=O.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (370.80 mM; Need ultrasonic)

    DMSO : 100 mg/mL (370.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7080 mL 18.5398 mL 37.0796 mL
    5 mM 0.7416 mL 3.7080 mL 7.4159 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 140 mg/mL (519.11 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.70%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.7080 mL 18.5398 mL 37.0796 mL 92.6990 mL
    5 mM 0.7416 mL 3.7080 mL 7.4159 mL 18.5398 mL
    10 mM 0.3708 mL 1.8540 mL 3.7080 mL 9.2699 mL
    15 mM 0.2472 mL 1.2360 mL 2.4720 mL 6.1799 mL
    20 mM 0.1854 mL 0.9270 mL 1.8540 mL 4.6350 mL
    25 mM 0.1483 mL 0.7416 mL 1.4832 mL 3.7080 mL
    30 mM 0.1236 mL 0.6180 mL 1.2360 mL 3.0900 mL
    40 mM 0.0927 mL 0.4635 mL 0.9270 mL 2.3175 mL
    50 mM 0.0742 mL 0.3708 mL 0.7416 mL 1.8540 mL
    60 mM 0.0618 mL 0.3090 mL 0.6180 mL 1.5450 mL
    80 mM 0.0463 mL 0.2317 mL 0.4635 mL 1.1587 mL
    100 mM 0.0371 mL 0.1854 mL 0.3708 mL 0.9270 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    L-NAME hydrochloride
    Cat. No.:
    HY-18729A
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