1. Immunology/Inflammation Apoptosis
  2. NO Synthase Apoptosis
  3. 1400W Dihydrochloride

1400W Dihydrochloride 

Cat. No.: HY-18731 Purity: 99.94%
COA Handling Instructions

1400W dihydrochloride is the dihydrochloride form of 1400W (HY-18731). 1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a Kd value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation.

For research use only. We do not sell to patients.

1400W Dihydrochloride Chemical Structure

1400W Dihydrochloride Chemical Structure

CAS No. : 214358-33-5

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
5 mg USD 55 In-stock
10 mg USD 88 In-stock
50 mg USD 308 In-stock
100 mg USD 528 In-stock
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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of 1400W Dihydrochloride:

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  • Biological Activity

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Description

1400W dihydrochloride is the dihydrochloride form of 1400W (HY-18731). 1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a Kd value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation[1][2].

IC50 & Target

iNOS

 

In Vitro

1400W (60 μM, 1 h) reduces NO, 3-NT and MDA production in primary adult microglia, and prevents neuronal cell apoptosis in cerebral cortex[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1400W (0.1-10 mg/kg, s.c., once time) inhibits the leakage of rats ileum, with an EC50 of 0.16 mg/kg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

250.17

Formula

C10H17Cl2N3

CAS No.
Appearance

Solid

Color

White to pink

SMILES

CC(NCC1=CC=CC(CN)=C1)=N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (399.73 mM; Need ultrasonic)

DMSO : 20 mg/mL (79.95 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9973 mL 19.9864 mL 39.9728 mL
5 mM 0.7995 mL 3.9973 mL 7.9946 mL
10 mM 0.3997 mL 1.9986 mL 3.9973 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (399.73 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.94%

References
Animal Administration
[1]

Rats: The effects of 1400W on plasma leakage are assessed in rats by determining the leakage of [125I]human serum albumin from plasma into organs. 1400W (0.1-10 mg/kg, subcutaneous) is dissolved in isotonic saline and administered either concurrently with endotoxin or 3 h following LPS administration (E. coli LPS, 3 mg/kg intravenously). Plasma leakage is then assessed 1 or 5 h after delivery of 1400W[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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1400W Dihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
1400W Dihydrochloride
Cat. No.:
HY-18731
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