1. Immunology/Inflammation
  2. NO Synthase
  3. 1400W Dihydrochloride

1400W Dihydrochloride 

Cat. No.: HY-18731 Purity: 99.65%
Handling Instructions

1400W dihydrochloride is a potent and selective inhibitor of human inducible NO synthase with Ki values of 7 nM.

For research use only. We do not sell to patients.

1400W Dihydrochloride Chemical Structure

1400W Dihydrochloride Chemical Structure

CAS No. : 214358-33-5

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

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Description

1400W dihydrochloride is a potent and selective inhibitor of human inducible NO synthase with Ki values of 7 nM.

IC50 & Target

Ki: 7 nM (iNOS), 2 µM (nNOS), 50 µM (eNOS)[1]

In Vitro

1400W is a slow, tight binding inhibitor of human inducible nitric- oxide synthase (iNOS). The slow onset of inhibition by 1400W shows saturation kinetics with a maximal rate constant of 0.028 s-1 and a binding constant of 2.0 μM. Inhibition is dependent on the cofactor NADPH. 1400W is at least 5000-fold selective for iNOS versus eNOS. In contrast, inhibition of human neuronal NOS and endothelial NOS (eNOS) is relatively weaker, rapidly reversible, and competitive with L-arginine, with Ki values of 2 μM and 50 μM, respectively[1]. 1400W treatment inhibits iNOS expression without affecting nNOS or eNOS. 1400W also reduces NO, 3-NT and MDA production, and prevents neuronal cell apoptosis in cerebral cortex[2].

In Vivo

1400W potently (ED50=0.3 mg/kg) reduces the delayed vascular injury in rats attributable to LPS-induced iNOS but fails to exacerbate acute vascular leakage when given concurrently with LPS[1]. Administration of 1400W lowers NOx levels in all the experimental groups. In addition, lipid peroxidation, the percentage of apoptotic cells, and nitrated protein expression fall in the late post-hypoxia period (48 h and 5 days)[3].

Molecular Weight

250.17

Formula

C₁₀H₁₇Cl₂N₃

CAS No.

214358-33-5

SMILES

CC(NCC1=CC=CC(CN)=C1)=N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (79.95 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9973 mL 19.9864 mL 39.9728 mL
5 mM 0.7995 mL 3.9973 mL 7.9946 mL
10 mM 0.3997 mL 1.9986 mL 3.9973 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Animal Administration
[1]

Rats: The effects of 1400W on plasma leakage are assessed in rats by determining the leakage of [125I]human serum albumin from plasma into organs. 1400W (0.1-10 mg/kg, subcutaneous) is dissolved in isotonic saline and administered either concurrently with endotoxin or 3 h following LPS administration (E. coli LPS, 3 mg/kg intravenously). Plasma leakage is then assessed 1 or 5 h after delivery of 1400W[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.65%

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Keywords:

1400WNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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Product Name:
1400W Dihydrochloride
Cat. No.:
HY-18731
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