Cat. No.: HY-18731

1400W Dihydrochloride

29 Cited Publications

Customer Review

Based on 29 publication(s) in Google Scholar

1400W dihydrochloride is the dihydrochloride form of 1400W (HY-18730). 1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a K_d value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 214358-33-5
Formula: C₁₀H₁₇Cl₂N₃
Molecular Weight:250.17

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

1400W Dihydrochloride Chemical Structure

CAS No.: 214358-33-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (29)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

iNOS

All NO Synthase Isoforms :

In Vitro

1400W (60 μM, 1 h) reduces NO, 3-NT and MDA production in primary adult microglia, and prevents neuronal cell apoptosis in cerebral cortex^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1400W (0.1-10 mg/kg, s.c., once time) inhibits the leakage of rats ileum, with an EC₅₀ of 0.16 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 214358-33-5
Appearance Solid
Molecular Weight 250.17
Formula C₁₀H₁₇Cl₂N₃
Color White to pink
SMILES

CC(NCC1=CC=CC(CN)=C1)=N.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (29)

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Journal Impact Factor

Signal Transduct Target Ther

Oleic acid restores the impaired antitumor immunity of γδ-T cells induced by palmitic acid. [Abstract]

2025 Jul 3;10(1):209. PMID: 40603316

1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]

1400W dihydrochloride inhibited PA-induced LDH release in Vγ9Vδ2-T cells.

1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]

1400W dihydrochloride treatment effectively suppressed the induction of GSDMD cleavage and caspase 1 activation of Vγ9Vδ2-T cells following rhIFNγ stimulation under BSA or OA conditions.
Cell Metab

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]

2025 Jan 7;37(1):291-304.e9. PMID: 39413790

1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Jan 7;37(1):291-304.e9. [Abstract]

Effects of NOS inhibitors on phagocytosis efficiency in M0 and M1 BMDMs. The results showed that treatment with the NOS inhibitor 1400W dihydrochloride suppressed the phagocytic efficiency of activated M1 BMDMs, implying that NOS‑mediated arginine metabolism was crucial for macrophage phagocytosis.
Nat Commun

USP16 S-nitrosylation aggravates coronary microembolization-induced myocardial injury via repressing KDM1A-mediated glutathione homeostasis. [Abstract]

2025 Dec 3;17(1):255. PMID: 41339351
ACS Nano

Microneedles Loaded with Nitric-Oxide Driven Nanomotors Improve Force-Induced Efferocytosis Impairment and Sterile Inflammation by Revitalizing Macrophage Energy Metabolism. [Abstract]

2025 Mar 11;19(9):9390-9411. PMID: 40025734
Redox Biol

VEO-IBD NOX1 variant highlights a structural region essential for NOX/DUOX catalytic activity. [Abstract]

2023 Nov:67:102905. PMID: 37820403
Mol Cell

PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. [Abstract]

2020 Jan 2;77(1):95-107.e5. PMID: 31628042

1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]

HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were treated with the indicated concentration of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 μM). The S-nitrosylation detection assay was performed. Addition of a high dosage of exogenous BH4 led to an increased LTBP1 S-nitrosylation under hypoxia in HT29 cells with expression of PTPS R25Q. The effects of exogenous BH4 found here were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.

1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]

HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were added with indicated concentrations of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 mM). Cells were treated with cycloheximide (CHX; 30 mg/mL) for the indicated length of time at 6 h under hypoxia treatment. Immunoblotting and relative intensities of LTBP1 to beta-actin were analyzed. Addition of a high dosage of exogenous BH4 led to decreased LTBP1 stability and increased LTBP1 ubiquitination. The effects of exogenous BH4 were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.
Int J Biol Sci

Downregulation of Nitric Oxide Collaborated with Radiotherapy to Promote Anti-Tumor Immune Response via Inducing CD8+ T Cell Infiltration. [Abstract]

2020 Mar 5;16(9):1563-1574. PMID: 32226302
ISME J

Protozoan predation enhances stress resistance and antibiotic tolerance in Burkholderia cenocepacia by triggering the SOS response. [Abstract]

2024 Jan 8;18(1):wrae014. PMID: 38366016
J Transl Med

Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche. [Abstract]

2023 Nov 25;21(1):852. PMID: 38007452
Hum Reprod Open

Impaired PIEZO1 function drives uterine hypercontractility in adenomyosis-associated dysmenorrhea. [Abstract]

2026 Feb 17;2026(2):hoag013. PMID: 41836349
Cancer Immunol Res

CLK1 Promotes Myeloid-Derived Suppressor Cell Trafficking and Reprograms the Tumor Microenvironment by Activating Hippo/YAP Signaling in Colorectal Cancer. [Abstract]

2026 May 4;14(5):875-891. PMID: 41686262
Aging Cell

Aging-Associated Liver Sinusoidal Endothelial Cells Dysfunction Aggravates the Progression of Metabolic Dysfunction-Associated Steatotic Liver Disease. [Abstract]

2025 May;24(5):e14502. PMID: 39912563
Cell Rep

Blockage of citrate export prevents TCA cycle fragmentation via Irg1 inactivation. [Abstract]

2022 Feb 15;38(7):110391. PMID: 35172156
Int Immunopharmacol

A novel triazole derivative ameliorates ethanol-induced gastric ulcer via a NOS2-centered inhibition of the AGE-RAGE pathway. [Abstract]

2026 Mar 1:172:116240. PMID: 41576570
Mucosal Immunol

Deficiency of immunoglobulin IgSF6 enhances antibacterial effects by promoting ER stress and the inflammatory response in intestinal macrophages. [Abstract]

2024 Apr;17(2):288-302. PMID: 38387824
Neuropharmacology

Microglial-derived nitric oxide regulates amygdala synaptic plasticity to drive chronic pain and depression induced by lumbar disc herniation. [Abstract]

2025 Dec 1:280:110662. PMID: 40887007
Mol Cell Biochem

Nitric oxide induces apoptosis of human primary melanocytes by regulating calcium homeostasis via VDAC1. [Abstract]

2025 Aug 3. PMID: 40753527
J Physiol

The protective effect of 1400W against ischaemia and reperfusion injury is countered by transient medullary kidney endothelial dysregulation. [Abstract]

2024 Jul 26. PMID: 39057844
iScience

Nosip is a potential therapeutic target in hepatocellular carcinoma cells. [Abstract]

2023 Jul 10;26(8):107353. PMID: 37529099
Skelet Muscle

Oxidative stress-induced premature senescence and aggravated denervated skeletal muscular atrophy by regulating progerin-p53 interaction. [Abstract]

2022 Jul 29;12(1):19. PMID: 35906707
Microvasc Res

Hypoglycaemia aggravates impaired endothelial-dependent vasodilation in diabetes by suppressing endothelial nitric oxide synthase activity and stimulating inducible nitric oxide synthase expression. [Abstract]

2023 Mar:146:104468. PMID: 36513147
Biochem Biophys Res Commun

A novel genetically encoded indicator for deciphering cytosolic and mitochondrial nitric oxide in live cells. [Abstract]

2025 Feb 16:749:151345. PMID: 39862720
Int J Ophthalmol

Dynamic changes of inducible nitric oxide synthase expression in rat's retina and its role on blood-retinal barrier injury after acute high intraocular pressure. [Abstract]

2022 Jul 18;15(7):1053-1061. PMID: 35919326
bioRxiv

2026 Jun 4.
bioRxiv

2025 Feb 17.
University of Jena

2023 Dec.
Biomed Pharmacother

Oridonin attenuates lung inflammation and fibrosis in silicosis via covalent targeting iNOS. [Abstract]

2022 Sep;153:113532. PMID: 36076611
Biomed Pharmacother

Dendrobium officinale polysaccharides attenuate uropathogenic Escherichia coli (UPEC)-induced pyroptosis in macrophage cells. [Abstract]

2022 Jul;151:113098. PMID: 35594714
Sci Total Environ

Manganese induces autophagy dysregulation: The role of S-nitrosylation in regulating autophagy related proteins in vivo and in vitro. [Abstract]

2020 Jan 1:698:134294. PMID: 31783459

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (399.73 mM; Need ultrasonic)

DMSO : 20 mg/mL (79.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9973 mL

19.9864 mL

39.9728 mL

5 mM

0.7995 mL

3.9973 mL

7.9946 mL

10 mM

0.3997 mL

1.9986 mL

3.9973 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (399.73 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protocol

Animal Administration^[1]

Rats: The effects of 1400W on plasma leakage are assessed in rats by determining the leakage of [¹²⁵I]human serum albumin from plasma into organs. 1400W (0.1-10 mg/kg, subcutaneous) is dissolved in isotonic saline and administered either concurrently with endotoxin or 3 h following LPS administration (E. coli LPS, 3 mg/kg intravenously). Plasma leakage is then assessed 1 or 5 h after delivery of 1400W^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Garvey EP, et al. 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem. 1997 Feb 21;272(8):4959-63. [Content Brief]

[2]. Shi Q, et al. 1400W ameliorates acute hypobaric hypoxia/reoxygenation-induced cognitive deficits by suppressing the induction of inducible nitric oxide synthase in rat cerebral cortex microglia. Behav Brain Res. 2017 Feb 15;319:188-199. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.9973 mL	19.9864 mL	39.9728 mL	99.9320 mL
	5 mM	0.7995 mL	3.9973 mL	7.9946 mL	19.9864 mL
	10 mM	0.3997 mL	1.9986 mL	3.9973 mL	9.9932 mL
	15 mM	0.2665 mL	1.3324 mL	2.6649 mL	6.6621 mL
	20 mM	0.1999 mL	0.9993 mL	1.9986 mL	4.9966 mL
	25 mM	0.1599 mL	0.7995 mL	1.5989 mL	3.9973 mL
	30 mM	0.1332 mL	0.6662 mL	1.3324 mL	3.3311 mL
	40 mM	0.0999 mL	0.4997 mL	0.9993 mL	2.4983 mL
	50 mM	0.0799 mL	0.3997 mL	0.7995 mL	1.9986 mL
	60 mM	0.0666 mL	0.3331 mL	0.6662 mL	1.6655 mL
H₂O	80 mM	0.0500 mL	0.2498 mL	0.4997 mL	1.2492 mL
H₂O	100 mM	0.0400 mL	0.1999 mL	0.3997 mL	0.9993 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

1400W Dihydrochloride

29 Cited Publications

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1400W Dihydrochloride

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Neuroprotective Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Targeted Diversity Library

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Highly Selective Inhibitors Library

Cell Death Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

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Anti-Aging Compound Library Mini

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) 1400W Dihydrochloride

All NO Synthase Isoforms

Biological Activity

Chemical Information

Publications (29)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

1400W Dihydrochloride

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Neuroprotective Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Targeted Diversity Library

Human Metabolite Library

Anti-Pulmonary Fibrosis Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Highly Selective Inhibitors Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Anti-Aging Compound Library Mini

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) 1400W Dihydrochloride

All NO Synthase Isoforms

Biological Activity

Chemical Information

Publications (29)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

1400W Dihydrochloride Related Classifications

HY-18731 Related Classifications