1400W Dihydrochloride

Name: 1400W Dihydrochloride
Brand: MedChemExpress
SKU: HY-18731
Rating: 5.0 (28 reviews)

Cat. No.: HY-18731 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

1400W dihydrochloride is the dihydrochloride form of 1400W (HY-18730). 1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a K_d value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation.

For research use only. We do not sell to patients.

CAS No. : 214358-33-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 28 publication(s) in Google Scholar

Other Forms of 1400W Dihydrochloride:

1400W In-stock
1400W Dihydrochloride (Standard) Get quote

28 Publications Citing Use of MCE 1400W Dihydrochloride

Cell Proliferation/Viability Assay

Flow Cytometry

: 1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]; 1400W dihydrochloride inhibited PA-induced LDH release in Vγ9Vδ2-T cells.

: 1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]; 1400W dihydrochloride treatment effectively suppressed the induction of GSDMD cleavage and caspase 1 activation of Vγ9Vδ2-T cells following rhIFNγ stimulation under BSA or OA conditions.

: 1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Jan 7;37(1):291-304.e9. [Abstract]; Effects of NOS inhibitors on phagocytosis efficiency in M0 and M1 BMDMs. The results showed that treatment with the NOS inhibitor 1400W dihydrochloride suppressed the phagocytic efficiency of activated M1 BMDMs, implying that NOS‑mediated arginine metabolism was crucial for macrophage phagocytosis.

: 1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]; HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were treated with the indicated concentration of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 μM). The S-nitrosylation detection assay was performed. Addition of a high dosage of exogenous BH4 led to an increased LTBP1 S-nitrosylation under hypoxia in HT29 cells with expression of PTPS R25Q. The effects of exogenous BH4 found here were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.

: 1400W Dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]; HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were added with indicated concentrations of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 mM). Cells were treated with cycloheximide (CHX; 30 mg/mL) for the indicated length of time at 6 h under hypoxia treatment. Immunoblotting and relative intensities of LTBP1 to beta-actin were analyzed. Addition of a high dosage of exogenous BH4 led to decreased LTBP1 stability and increased LTBP1 ubiquitination. The effects of exogenous BH4 were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

iNOS

In Vitro

1400W (60 μM, 1 h) reduces NO, 3-NT and MDA production in primary adult microglia, and prevents neuronal cell apoptosis in cerebral cortex^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1400W (0.1-10 mg/kg, s.c., once time) inhibits the leakage of rats ileum, with an EC₅₀ of 0.16 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

250.17

Formula

C₁₀H₁₇Cl₂N₃

CAS No.

214358-33-5

Appearance

Solid

Color

White to pink

SMILES

CC(NCC1=CC=CC(CN)=C1)=N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (399.73 mM; Need ultrasonic)

DMSO : 20 mg/mL (79.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9973 mL	19.9864 mL	39.9728 mL
5 mM	0.7995 mL	3.9973 mL	7.9946 mL
10 mM	0.3997 mL	1.9986 mL	3.9973 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (7.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (399.73 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB) HNMR (241 KB) RP-HPLC (206 KB)

Handling Instructions (2659 KB)

References

[1]. Garvey EP, et al. 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem. 1997 Feb 21;272(8):4959-63. [Content Brief]

[2]. Shi Q, et al. 1400W ameliorates acute hypobaric hypoxia/reoxygenation-induced cognitive deficits by suppressing the induction of inducible nitric oxide synthase in rat cerebral cortex microglia. Behav Brain Res. 2017 Feb 15;319:188-199. [Content Brief]

Animal Administration
^[1]

Rats: The effects of 1400W on plasma leakage are assessed in rats by determining the leakage of [¹²⁵I]human serum albumin from plasma into organs. 1400W (0.1-10 mg/kg, subcutaneous) is dissolved in isotonic saline and administered either concurrently with endotoxin or 3 h following LPS administration (E. coli LPS, 3 mg/kg intravenously). Plasma leakage is then assessed 1 or 5 h after delivery of 1400W^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Garvey EP, et al. 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem. 1997 Feb 21;272(8):4959-63. [Content Brief]

[2]. Shi Q, et al. 1400W ameliorates acute hypobaric hypoxia/reoxygenation-induced cognitive deficits by suppressing the induction of inducible nitric oxide synthase in rat cerebral cortex microglia. Behav Brain Res. 2017 Feb 15;319:188-199. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.9973 mL	19.9864 mL	39.9728 mL	99.9320 mL
	5 mM	0.7995 mL	3.9973 mL	7.9946 mL	19.9864 mL
	10 mM	0.3997 mL	1.9986 mL	3.9973 mL	9.9932 mL
	15 mM	0.2665 mL	1.3324 mL	2.6649 mL	6.6621 mL
	20 mM	0.1999 mL	0.9993 mL	1.9986 mL	4.9966 mL
	25 mM	0.1599 mL	0.7995 mL	1.5989 mL	3.9973 mL
	30 mM	0.1332 mL	0.6662 mL	1.3324 mL	3.3311 mL
	40 mM	0.0999 mL	0.4997 mL	0.9993 mL	2.4983 mL
	50 mM	0.0799 mL	0.3997 mL	0.7995 mL	1.9986 mL
	60 mM	0.0666 mL	0.3331 mL	0.6662 mL	1.6655 mL
H₂O	80 mM	0.0500 mL	0.2498 mL	0.4997 mL	1.2492 mL
H₂O	100 mM	0.0400 mL	0.1999 mL	0.3997 mL	0.9993 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.