1400W (Synonyms: W1400)

Name: 1400W
Brand: MedChemExpress
SKU: HY-18730
Rating: 5.0 (28 reviews)

Cat. No.: HY-18730 Purity: 98.49%: Data Sheet
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1400W is a slow, tight binding, and highly selective inducible nitric-oxide synthase (iNOS) inhibitor, with a K_d value ≤ 7 nM. 1400W inhibits iNOS induction in microglial cells, and reduces generation of NO, thereby mitigating oxidative stress and neuronal cell apoptosis in the rat cerebral cortex, and improving the spatial memory dysfunction caused by acute hypobaric hypoxia-reoxygenation.

For research use only. We do not sell to patients.

CAS No. : 180001-34-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 28 publication(s) in Google Scholar

Other Forms of 1400W:

1400W Dihydrochloride In-stock

28 Publications Citing Use of MCE 1400W

Cell Proliferation/Viability Assay

Flow Cytometry

: 1400W purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]; 1400W dihydrochloride inhibited PA-induced LDH release in Vγ9Vδ2-T cells.

: 1400W purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Jul 3;10(1):209. [Abstract]; 1400W dihydrochloride treatment effectively suppressed the induction of GSDMD cleavage and caspase 1 activation of Vγ9Vδ2-T cells following rhIFNγ stimulation under BSA or OA conditions.

: 1400W purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Jan 7;37(1):291-304.e9. [Abstract]; Effects of NOS inhibitors on phagocytosis efficiency in M0 and M1 BMDMs. The results showed that treatment with the NOS inhibitor 1400W dihydrochloride suppressed the phagocytic efficiency of activated M1 BMDMs, implying that NOS‑mediated arginine metabolism was crucial for macrophage phagocytosis.

: 1400W purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]; HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were treated with the indicated concentration of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 μM). The S-nitrosylation detection assay was performed. Addition of a high dosage of exogenous BH4 led to an increased LTBP1 S-nitrosylation under hypoxia in HT29 cells with expression of PTPS R25Q. The effects of exogenous BH4 found here were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.

: 1400W purchased from MedChemExpress. Usage Cited in: Mol Cell. 2020 Jan 2;77(1):95-107.e5. [Abstract]; HT29 cells with depleted PTPS and reconstituted expression of rPTPS R25Q were cultured for 12 h under hypoxia and were added with indicated concentrations of exogenous BH4 in the presence or absence of 1400W Dihydrochloride (100 mM). Cells were treated with cycloheximide (CHX; 30 mg/mL) for the indicated length of time at 6 h under hypoxia treatment. Immunoblotting and relative intensities of LTBP1 to beta-actin were analyzed. Addition of a high dosage of exogenous BH4 led to decreased LTBP1 stability and increased LTBP1 ubiquitination. The effects of exogenous BH4 were abolished when cells were treated with the iNOS inhibitor 1400W Dihydrochloride.

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: 2 µM (nNOS), 50 µM (eNOS)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	EC₅₀	> 100 μM Compound: 1400W	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 5 days by PrestoBlue reagent based assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 5 days by PrestoBlue reagent based assay	[PMID: 36641860]
N9	IC₅₀	2.2 μM Compound: N-(3-aminomethyl)benzylacetamidine	Inhibitory effect of compound on the accumulation of NO2 in the culture media of N9 cells in response to LPS/IFN-gamma Inhibitory effect of compound on the accumulation of NO2 in the culture media of N9 cells in response to LPS/IFN-gamma	[PMID: 15013012]
N9	IC₅₀	3.6 μM Compound: 1400W	Antiinflammatory activity in LPS/IFN-gamma-stimulated mouse N9 cells assessed as inhibition of nitric oxide production pretreated 1 hr before LPS/IFNgamma challenge Antiinflammatory activity in LPS/IFN-gamma-stimulated mouse N9 cells assessed as inhibition of nitric oxide production pretreated 1 hr before LPS/IFNgamma challenge	[PMID: 12662101]
N9	IC₅₀	6.3 μM Compound: 1400W	Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation after 24 hrs Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation after 24 hrs	[PMID: 15568764]
RAW	IC₅₀	5.2 μM Compound: N-(3-aminomethyl)benzylacetamidine	Inhibition of 1ug/ml LPS-stimulated nitirc oxide accumulation in RAW 264 cells after 24 hrs Inhibition of 1ug/ml LPS-stimulated nitirc oxide accumulation in RAW 264 cells after 24 hrs	[PMID: 17320246]
RAW	IC₅₀	6.1 μM Compound: N-(3-aminomethyl)benzylacetamidine	Inhibitory effect of compound on the accumulation of NO2 in the culture media of RAW 264.7 cells in response to LPS Inhibitory effect of compound on the accumulation of NO2 in the culture media of RAW 264.7 cells in response to LPS	[PMID: 15013012]
RAW264.7	IC₅₀	0.2 μM Compound: 1400W	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23232148]
RAW264.7	IC₅₀	0.3 μM Compound: 1400W	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs by fluorimetry Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs by fluorimetry	[PMID: 11678654]
RAW264.7	IC₅₀	1.5 μM Compound: 1400W	Inhibition of LPS-induced nitric oxide generation in mouse RAW264.7 cells pretreated 1 hr before LPS challenge by Griess method Inhibition of LPS-induced nitric oxide generation in mouse RAW264.7 cells pretreated 1 hr before LPS challenge by Griess method	[PMID: 19278854]
RAW264.7	IC₅₀	2.3 μM Compound: 1400W	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production pretreated 1 hr before LPS challenge Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production pretreated 1 hr before LPS challenge	[PMID: 12662101]
RAW264.7	IC₅₀	2.7 μM Compound: 1400W	Inhibition of LPS-induced nitric oxide accumulation in mouse RAW264.7 cells Inhibition of LPS-induced nitric oxide accumulation in mouse RAW264.7 cells	[PMID: 19699097]
RAW264.7	IC₅₀	2.9 μM Compound: 1400W	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs	[PMID: 15568764]
RAW264.7	IC₅₀	3.1 μM Compound: 1400W	Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as effect on NO production pretreated 1 hr before LPS challenge measured after 24 hrs Antiinflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as effect on NO production pretreated 1 hr before LPS challenge measured after 24 hrs	[PMID: 11908984]
RAW264.7	IC₅₀	3.78 μM Compound: 1400W	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells	[PMID: 17988874]
RAW264.7	IC₅₀	47.47 μM Compound: 1400W	Cytotoxicity against mouse RAW264.7 cells assessed as cell viability after 24 hrs by MTS assay Cytotoxicity against mouse RAW264.7 cells assessed as cell viability after 24 hrs by MTS assay	[PMID: 23232148]
RAW264.7	IC₅₀	> 10 μM Compound: 1400W	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as PGE2 accumulation after 20 hrs by RIA Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as PGE2 accumulation after 20 hrs by RIA	[PMID: 11678654]
THP-1	IC₅₀	0.33 μM Compound: 1, W1400	Inhibition of iNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay Inhibition of iNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay	[PMID: 19203270]
THP-1	IC₅₀	1100 μM Compound: 1, W1400	Inhibition of eNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay Inhibition of eNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay	[PMID: 19203270]

In Vitro

1400W (60 μM, 1 h) reduces NO, 3-NT and MDA production in primary adult microglia, and prevents neuronal cell apoptosis in cerebral cortex^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

1400W (0.1-10 mg/kg, s.c., once time) inhibits the leakage of rats ileum, with an EC₅₀ of 0.16 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

177.25

Formula

C₁₀H₁₅N₃

CAS No.

180001-34-7

Appearance

Solid

Color

White to off-white

SMILES

CC(NCC1=CC=CC(CN)=C1)=N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (564.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6417 mL	28.2087 mL	56.4175 mL
5 mM	1.1283 mL	5.6417 mL	11.2835 mL
10 mM	0.5642 mL	2.8209 mL	5.6417 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (28.21 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (28.21 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (28.21 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.49%

Select Batch:

Data Sheet (271 KB) SDS (392 KB)

COA (243 KB) HNMR (444 KB) RP-HPLC (259 KB) MS (67 KB)

Handling Instructions (2659 KB)

References

[1]. Garvey EP, et al. 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem. 1997 Feb 21;272(8):4959-63. [Content Brief]

[2]. Shi Q, et al. 1400W ameliorates acute hypobaric hypoxia/reoxygenation-induced cognitive deficits by suppressing the induction of inducible nitric oxide synthase in rat cerebral cortex microglia. Behav Brain Res. 2017 Feb 15;319:188-199. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6417 mL	28.2087 mL	56.4175 mL	141.0437 mL
	5 mM	1.1283 mL	5.6417 mL	11.2835 mL	28.2087 mL
	10 mM	0.5642 mL	2.8209 mL	5.6417 mL	14.1044 mL
	15 mM	0.3761 mL	1.8806 mL	3.7612 mL	9.4029 mL
	20 mM	0.2821 mL	1.4104 mL	2.8209 mL	7.0522 mL
	25 mM	0.2257 mL	1.1283 mL	2.2567 mL	5.6417 mL
	30 mM	0.1881 mL	0.9403 mL	1.8806 mL	4.7015 mL
	40 mM	0.1410 mL	0.7052 mL	1.4104 mL	3.5261 mL
	50 mM	0.1128 mL	0.5642 mL	1.1283 mL	2.8209 mL
	60 mM	0.0940 mL	0.4701 mL	0.9403 mL	2.3507 mL
	80 mM	0.0705 mL	0.3526 mL	0.7052 mL	1.7630 mL
	100 mM	0.0564 mL	0.2821 mL	0.5642 mL	1.4104 mL

1400W Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1400W180001-34-7W1400W 1400W-1400NO SynthaseApoptosisNitric oxide synthasesNOSiNOSneuronal NOSendothelial NOSmicroglialNOneuronal cellapoptosisInhibitorinhibitorinhibit

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1400W (Synonyms: W1400)

Customer Review

Other Forms of 1400W:

1400W Related Antibodies

28 Publications Citing Use of MCE 1400W

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Biological Activity

Purity & Documentation

References

Customer Review

1400W Related Antibodies

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Complete Stock Solution Preparation Table

1400W Related Classifications

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