Fangchinoline

Name: Fangchinoline
Brand: MedChemExpress
SKU: HY-N1372A
Rating: 5.0 (10 reviews)

Cat. No.: HY-N1372A Purity: 99.90%: Data Sheet
COA

SDS
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Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer.

For research use only. We do not sell to patients.

CAS No. : 436-77-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Fangchinoline:

Fangchinoline (Standard) Get quote

10 Publications Citing Use of MCE Fangchinoline

Cell Proliferation/Viability Assay

Flow Cytometry

Cell Migration/Invasion Assay

: Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]; CCK-8 examination of the viability of Fangchinoline (Fan)-treated NSCLC cells.

: Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]; NSCLC cells were stained with CFDA-SE and treated with Fangchinoline (Fan) (1.25, 2.5, 5 μM) for 24 h. The mean fluorescence intensity was detected by flow cytometry in the FITC channel.

: Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]; Cell viability was determined by the EdU assay treated with Fangchinoline (Fan) (1.25, 2.5, 5 μM).

: Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]; EMT-related biomarker detection revealed that Fangchinoline (Fan) (1.25, 2.5, 5 μM) significantly inhibited EMT dose-dependent.

: Fangchinoline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Sep 1:543:215783. [Abstract]; The effect of Fangchinoline (Fan) (1.25, 2.5, 5 μM, 24, 48 h) on cell migration and invasion was assessed with the wound healing assay.

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HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing^[1]. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK^[2]. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer^[3].

IC₅₀ & Target

HIV-1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 14	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 23621840]
HEK-293T	CC₅₀	> 1 μM Compound: 2; fan	Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay	[PMID: 37043739]
HEL	IC₅₀	22.71 μM Compound: Fangchinoline	Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
HL-60	IC₅₀	> 10 μM Compound: 14	Cytotoxicity against human HL60 cells after 48 hrs by MTS assay Cytotoxicity against human HL60 cells after 48 hrs by MTS assay	[PMID: 23621840]
HepG2	IC₅₀	9.08 μM Compound: Fangchinoline	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28057423]
K562	IC₅₀	5.94 μM Compound: Fangchinoline	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
KB	ED₅₀	9210 nM Compound: 20	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 9917283]
MCF7	IC₅₀	10.1 μM Compound: Fangchinoline	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 28057423]
MCF7	IC₅₀	17.41 μM Compound: Fangchinoline	Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
MCF7	IC₅₀	5 μM Compound: 14	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 23621840]
MDA-MB-231	IC₅₀	58.61 μM Compound: Fangchinoline	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
SMMC-7721	IC₅₀	> 10 μM Compound: 14	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay	[PMID: 23621840]
SW480	IC₅₀	> 10 μM Compound: 14	Cytotoxicity against human SW480 cells after 48 hrs by MTS assay Cytotoxicity against human SW480 cells after 48 hrs by MTS assay	[PMID: 23621840]
U-937	IC₅₀	> 50 μM Compound: 15, NSC 77036	Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay	[PMID: 22766217]

In Vitro

Fangchinoline (2.5-40 μM; 24-96 hours) inhibits both T24 and 5637 cells in dose-dependent manner, the IC₅₀ values of Fangchinoline in T24 cells are 19.0 μM (24 h), 12.0 μM (48 h) and 7.57 μM (72 h), and 11.9 μM (24 h), 9.92 μM (48 h) and 7.13 μM (72 h) in 5637 cells^[1].
Fangchinoline (5 μM; 24 hours) induces a significant increase in the LC3-II/LC3-I ratio and a decrease in p62 in both T24 and 5637 cells, and causes a significant increase in the cleavage of caspase-3^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	T24 and 5637 cells
Concentration:	2.5 µM; 5 µM; 10 µM; 20 µM; 30 µM; 40 µM
Incubation Time:	24 hours; 48 hours; 96 hours
Result:	Inhibited both T24 and 5637 cells proliferation.

Western Blot Analysis^[3]

Cell Line:	T24 and 5637 cells
Concentration:	5 µM
Incubation Time:	24 hours
Result:	Incresed LC3-II/LC3-I ratio and the cleavage of caspase-3.

Molecular Weight

608.72

Formula

C₃₇H₄₀N₂O₆

CAS No.

436-77-1

Appearance

Solid

Color

White to off-white

SMILES

OC1=C(OC2=CC([C@]3([H])CC(C=C4)=CC=C4O5)=C(CCN3C)C=C2OC)C([C@](N6C)([H])CC7=CC=C(OC)C5=C7)=C(CC6)C=C1OC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (82.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6428 mL	8.2140 mL	16.4279 mL
5 mM	0.3286 mL	1.6428 mL	3.2856 mL
10 mM	0.1643 mL	0.8214 mL	1.6428 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (285 KB) SDS (392 KB)

COA (263 KB) HNMR (205 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

References

[1]. Wan Z, et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One. 2012;7(6):e39225. [Content Brief]

[2]. Guo B, et al. Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549. J Drug Target. 2015 Apr;23(3):266-74. [Content Brief]

[3]. Fan B, et al. Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer. Cell Physiol Biochem. 2017;43(3):1003-1011. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6428 mL	8.2140 mL	16.4279 mL	41.0698 mL
	5 mM	0.3286 mL	1.6428 mL	3.2856 mL	8.2140 mL
	10 mM	0.1643 mL	0.8214 mL	1.6428 mL	4.1070 mL
	15 mM	0.1095 mL	0.5476 mL	1.0952 mL	2.7380 mL
	20 mM	0.0821 mL	0.4107 mL	0.8214 mL	2.0535 mL
	25 mM	0.0657 mL	0.3286 mL	0.6571 mL	1.6428 mL
	30 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3690 mL
	40 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0267 mL
	50 mM	0.0329 mL	0.1643 mL	0.3286 mL	0.8214 mL
	60 mM	0.0274 mL	0.1369 mL	0.2738 mL	0.6845 mL
	80 mM	0.0205 mL	0.1027 mL	0.2053 mL	0.5134 mL

Fangchinoline Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fangchinoline436-77-1HIVFAKApoptosisAutophagyHuman immunodeficiency virusPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinaseInhibitorinhibitorinhibit

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Fangchinoline

Customer Review

Other Forms of Fangchinoline:

Fangchinoline Related Antibodies

10 Publications Citing Use of MCE Fangchinoline

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Fangchinoline Related Antibodies

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Complete Stock Solution Preparation Table

Fangchinoline Related Classifications

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