1. Anti-infection
    Protein Tyrosine Kinase/RTK
    Apoptosis
    Autophagy
  2. HIV
    FAK
    Apoptosis
    Autophagy
  3. Fangchinoline

Fangchinoline 

Cat. No.: HY-N1372A Purity: 99.92%
Handling Instructions

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer.

For research use only. We do not sell to patients.

Fangchinoline Chemical Structure

Fangchinoline Chemical Structure

CAS No. : 436-77-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 67 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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50 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Fangchinoline is isolated from Stephania tetrandra with extensive biological activities, such as enhancing immunity, anti-inflammatory sterilization and anti-atherosclerosis. Fangchinoline, a novel HIV-1 inhibitor, inhibits HIV-1 replication by impairing gp160 proteolytic processing[1]. Fangchinoline targets Focal adhesion kinase (FAK) and suppresses FAK-mediated signaling pathway in tumor cells which highly expressed FAK[2]. Fangchinoline induces apoptosis and adaptive autophagy in bladder cancer[3].

IC50 & Target

HIV-1 replication[1]; Focal adhesion kinase (FAK)[2]; apoptosis; autophagy[3]

In Vitro

Fangchinoline (2.5-40 µM; 24-96 hours) inhibits both T24 and 5637 cells in dose-dependent manner, the IC50 values of Fangchinoline in T24 cells are 19.0 µM (24 h), 12.0 µM (48 h) and 7.57 µM (72 h), and 11.9 µM (24 h), 9.92 µM (48 h) and 7.13 µM (72 h) in 5637 cells[1].
Fangchinoline (5 µM; 24 hours) induces a significant increase in the LC3-II/LC3-I ratio and a decrease in p62 in both T24 and 5637 cells, and causes a significant increase in the cleavage of caspase-3[1].

Cell Viability Assay[3]

Cell Line: T24 and 5637 cells
Concentration: 2.5 µM; 5 µM; 10 µM; 20 µM; 30 µM; 40 µM
Incubation Time: 24 hours; 48 hours; 96 hours
Result: Inhibited both T24 and 5637 cells proliferation.

Western Blot Analysis[3]

Cell Line: T24 and 5637 cells
Concentration: 5 µM
Incubation Time: 24 hours
Result: Incresed LC3-II/LC3-I ratio and the cleavage of caspase-3.
Molecular Weight

608.72

Formula

C₃₇H₄₀N₂O₆

CAS No.

436-77-1

SMILES

OC1=C(OC2=CC([[email protected]]3([H])CC(C=C4)=CC=C4O5)=C(CCN3C)C=C2OC)C([[email protected]](N6C)([H])CC7=CC=C(OC)C5=C7)=C(CC6)C=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (82.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6428 mL 8.2140 mL 16.4279 mL
5 mM 0.3286 mL 1.6428 mL 3.2856 mL
10 mM 0.1643 mL 0.8214 mL 1.6428 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.42 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

FangchinolineHIVFAKApoptosisAutophagyHuman immunodeficiency virusPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinaseInhibitorinhibitorinhibit

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Fangchinoline
Cat. No.:
HY-N1372A
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