7ACC1 (Synonyms: DEAC; Coumarin D 1421; D 1421)

Name: 7ACC1
Brand: MedChemExpress
SKU: HY-D0067
Rating: 5.0 (10 reviews)

Cat. No.: HY-D0067 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

7ACC1 (DEAC; Coumarin D 1421; D 1421) is a monocarboxylate transporter 1 (MCT-1)/MCT-4 specifc blocker. 7ACC1 attenuates renal cancer cell proliferation, migration, invasion and down-regulates the levels of MCT1/MCT4 expression and extracellular lactate. 7ACC1 is promising for research of cancers.

For research use only. We do not sell to patients.

CAS No. : 50995-74-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE 7ACC1

: 7ACC1 purchased from MedChemExpress. Usage Cited in: Cancer Med. 2018 Sep;7(9):4690-4700. [Abstract]; The protein expression of MCT4 in the murine breast cancer cell line 4T1 treated with 7ACC1 (0.1 mM) or transfected with different ShMCT4 vectors.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Monocarboxylate Transporter Isoform Specific Products:

View All Isoforms

MCT1 MCT2 MCT4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

MCT1

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	> 100 μM Compound: 7	Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 34626786]
HepG2	IC₅₀	> 100 μM Compound: 10	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28666736]
MCF-10A	IC₅₀	> 100 μM Compound: 7	Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 34626786]
MCF7	IC₅₀	> 100 μM Compound: 10	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 28666736]
MDA-MB-231	IC₅₀	> 100 μM Compound: 10	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 28666736]
MDA-MB-231	IC₅₀	> 100 μM Compound: 7	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 34626786]
MDA-MB-231	IC₅₀	> 50 μM Compound: 11	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 37602711]
SK-HEP1	IC₅₀	> 100 μM Compound: 10	Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay	[PMID: 28666736]
SiHa	EC₅₀	1.8 μM Compound: 17	Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay	[PMID: 24095010]
SiHa	IC₅₀	0.86 μM Compound: 17	Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting	[PMID: 24095010]
SiHa	IC₅₀	250 nM Compound: 17	Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting	[PMID: 24095010]
WiDr	IC₅₀	> 100 μM Compound: 7	Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 34626786]

In Vitro

7ACC1 (10 μM, 0-96 h) attenuates the viability, decreases cell migration and invasion in co-culturing conditions of 786-O cells and HUVECs^[1].
7ACC1 (10 μM, 24 h) inhibits the protein expressions of MCT1 and MCT4, down-regulates the extracellular lactate levels in co-culturing conditions of 786-O cells and HUVECs^[1].
7ACC1 (0-1 μM, 24 h) inhibit the influx but not the efflux of lactate in cancer cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	786-O cells and HUVECs
Concentration:	10 µM
Incubation Time:	24 h
Result:	Attenuated the protein expressions of MCT1 and MCT4 in co-culturing of 786-O cells and HUVECs.

In Vivo

7ACC1 (3 mg/kg, i.p., daily for 12 days) inhibits the growth of MCT-expressing cervix SiHa, colorectal HCT116 and breast MCF-7 tumors in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	mice bearing tumors, including cervix cancer SiHa xenografts but also tumors derived from human colon cancer cells HCT116 and bladder cancer cells UM-UC3^[2]
Dosage:	3 mg/kg
Administration:	i.p., daily for 12 days
Result:	Led to significant SiHa and HCT116 tumor growth delays in mice bearing tumors.

Molecular Weight

261.27

Formula

C₁₄H₁₅NO₄

CAS No.

50995-74-9

Appearance

Solid

Color

Orange to red

Emission (Em)

445

Excitation (Ex)

345

SMILES

O=C(C1=CC2=CC=C(N(CC)CC)C=C2OC1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (127.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8275 mL	19.1373 mL	38.2746 mL
5 mM	0.7655 mL	3.8275 mL	7.6549 mL
10 mM	0.3827 mL	1.9137 mL	3.8275 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (248 KB) HNMR (236 KB) RP-HPLC (256 KB)

Handling Instructions (2659 KB)

References

[1]. Guo C, et al. Monocarboxylate transporter 1 and monocarboxylate transporter 4 in cancer-endothelial co-culturing microenvironments promote proliferation, migration, and invasion of renal cancer cells. Cancer Cell Int. 2019 Jun 28;19:170. [Content Brief]

[2]. Draoui N, et al. Antitumor activity of 7-aminocarboxycoumarin derivatives, a new class of potent inhibitors of lactate influx but not efflux. Mol Cancer Ther. 2014 Jun;13(6):1410-8. [Content Brief]

[3]. Mohamed F A, et al. Mitochondrial Pyruvate Carrier Inhibition Mitigates Murine Chronic Graft Versus Host Disease By Attenuating the Germinal Center Reaction[J]. Blood, 2023, 142: 459.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8275 mL	19.1373 mL	38.2746 mL	95.6865 mL
	5 mM	0.7655 mL	3.8275 mL	7.6549 mL	19.1373 mL
	10 mM	0.3827 mL	1.9137 mL	3.8275 mL	9.5686 mL
	15 mM	0.2552 mL	1.2758 mL	2.5516 mL	6.3791 mL
	20 mM	0.1914 mL	0.9569 mL	1.9137 mL	4.7843 mL
	25 mM	0.1531 mL	0.7655 mL	1.5310 mL	3.8275 mL
	30 mM	0.1276 mL	0.6379 mL	1.2758 mL	3.1895 mL
	40 mM	0.0957 mL	0.4784 mL	0.9569 mL	2.3922 mL
	50 mM	0.0765 mL	0.3827 mL	0.7655 mL	1.9137 mL
	60 mM	0.0638 mL	0.3190 mL	0.6379 mL	1.5948 mL
	80 mM	0.0478 mL	0.2392 mL	0.4784 mL	1.1961 mL
	100 mM	0.0383 mL	0.1914 mL	0.3827 mL	0.9569 mL

7ACC1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

7ACC1 (Synonyms: DEAC; Coumarin D 1421; D 1421)

Customer Review

7ACC1 Related Antibodies

10 Publications Citing Use of MCE 7ACC1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Monocarboxylate Transporter Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

7ACC1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

7ACC1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report