2. Others
  3. mRNA Biochemical Assay Reagents
  4. Firefly luciferase mRNA-LNP

Firefly luciferase mRNA-LNP is a lipid nanoparticle (LNP) containing firefly luciferase mRNA. Firefly luciferase mRNA-LNP exhibits a stable nanostructure, in which LNP plays a key role in effectively protecting and transporting mRNA to cells. Luciferase is a bioluminescent reporter gene for gene regulation and functional studies. Firefly Luciferase mRNA-LNP expresses firefly luciferase protein after entering the cells, which is often used for promoter activity detection or dual fluorescent molecular complementation experiments. Firefly luciferase mRNA-LNP is studied in research for RNA delivery, cell viability, and translation efficiency.

For research use only. We do not sell to patients.

Size Price Stock Quantity
100 μg In-stock
500 μg In-stock

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Firefly luciferase mRNA-LNP Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Firefly luciferase mRNA-LNP

In Vivo Imaging

    Firefly luciferase mRNA-LNP purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Mar 28;11(13):eads2295.  [Abstract]

    Representative in vivo imaging system images of major organs in SCI mice after being administered with firefly luciferase (FLuc) mRNA-LNPs (0.5 mg mRNA/kg). From left to right are the heart, liver, spleen, lung, two kidneys, and spinal cord. iv, intravenous; ip, intraperitoneal.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Firefly luciferase mRNA-LNP is a lipid nanoparticle (LNP) containing firefly luciferase mRNA. Firefly luciferase mRNA-LNP exhibits a stable nanostructure, in which LNP plays a key role in effectively protecting and transporting mRNA to cells. Luciferase is a bioluminescent reporter gene for gene regulation and functional studies. Firefly Luciferase mRNA-LNP expresses firefly luciferase protein after entering the cells, which is often used for promoter activity detection or dual fluorescent molecular complementation experiments. Firefly luciferase mRNA-LNP is studied in research for RNA delivery, cell viability, and translation efficiency[1][2].

    In Vitro

    Firefly luciferase mRNA-LNP (5-20 ng, 24 h) significantly increases the transfection efficiency dose-dependently in HepG2 cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Firefly luciferase mRNA-LNP Related Antibodies
    In Vivo

    Firefly luciferase mRNA-LNP (2 μg, i.v., single dose) allows total luminescent flux in C57BL/6 mice[3].
    Firefly luciferase mRNA-LNP (1 μg, i.m., on day 1 and day 21) is formulated with either ALC-0315 or SM-102, and SM-102 has 60% higher expression level of luciferase protein than ALC-0315 and cKK-E12 in BALB/c mice[4].
    Firefly luciferase mRNA-LNP (5-20 μg, i.m., single dose) induces activation of Aif1 in the hypothalamus but not in the prefrontal cortex of adult Balb/c male mice[5].
    Firefly luciferase mRNA-LNP (5 μg, i.m./i.v./i.p./i.t., single dose) results in a large portion of the luciferase activity as well as high levels of protein production in the liver, and significant bioluminescent signal in lungs and muscles of BALB/c mouse model[6].
    Firefly luciferase mRNA-LNP (5 μg, i.d./s.c., single dose) results in protein production only at the site of injection in BALB/c mouse model[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female BALB/c mice aged 6 weeks[1]
    Dosage: 0.1, 1.0, 5.0 μg
    Administration: Intradermal injection (i.d.)/Intraperitoneal injection (i.p.)/Subcutaneous injection (s.c.)/Intramuscular injection (i.m.)/Intravenous injection (i.v.)/Intratracheal injection (i.t.)
    Result: Could not measure signal when mice were injected with 0.1 μg of mRNA-LNPs.
    Resulted in the highest amount of peak protein production and the shortest duration of expression with intravenous delivery.
    Did not result in measurable luciferase expression in the liver or other anatomical sites with subcutaneous delivery.
    Resulted in the longest duration of translation with intramuscular and intradermal delivery.
    Allowed saturation of the uptake or translational capacity of the intradermal space with intradermal delivery.
    Observed linear dose response with intravenous injection.
    Led to similar levels of peak translation at 4 h post injection with all three doses, but the increased doses extended the duration of translation from 5 (1.0 μg) to 10 (5.0 μg) days.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Firefly luciferase mRNA-LNP]
    Shipping

    Shipping with dry ice.
    Storage

    -80°C
    Purity & Documentation

    SDS (251 KB)

    COA (240 KB)

    Handling Instructions (2659 KB)
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Firefly luciferase mRNA-LNP Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Firefly luciferase mRNA-LNPmRNABiochemical Assay Reagentsmessenger RNAlipid nanoparticlebioluminescenttranslation efficiencyInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Firefly luciferase mRNA-LNP
    Cat. No.:
    HY-153229
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.