1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. CXCL14
  6. CXCL14/BRAK Protein, Mouse (His)

CXCL14/BRAK Protein, Mouse (His)

Cat. No.: HY-P71887A
COA Handling Instructions

CXCL14/BRAK protein selectively attracts CESS B cells and THP-1 monocytes without affecting T cells. Its specific chemical attraction emphasizes its role in mediating B cell and monocyte migration, contributing to immune responses within the microenvironment. CXCL14/BRAK Protein, Mouse (His) is the recombinant mouse-derived CXCL14/BRAK protein, expressed by E. coli , with N-6*His labeled tag. The total length of CXCL14/BRAK Protein, Mouse (His) is 77 a.a., with molecular weight of ~13 kDa.

For research use only. We do not sell to patients.

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Description

CXCL14/BRAK protein selectively attracts CESS B cells and THP-1 monocytes without affecting T cells. Its specific chemical attraction emphasizes its role in mediating B cell and monocyte migration, contributing to immune responses within the microenvironment. CXCL14/BRAK Protein, Mouse (His) is the recombinant mouse-derived CXCL14/BRAK protein, expressed by E. coli , with N-6*His labeled tag. The total length of CXCL14/BRAK Protein, Mouse (His) is 77 a.a., with molecular weight of ~13 kDa.

Background

CXCL14/BRAK protein functions as a chemotactic factor specifically attracting CESS B-cells and THP-1 monocytes, with no chemotactic effect on T-cells. This selective chemoattraction underscores its role in mediating the migration of B-cells and monocytes, contributing to immune responses within the microenvironment. The distinct chemotactic properties of CXCL14/BRAK highlight its specificity for certain immune cell types, suggesting a specialized role in regulating the recruitment and activation of B-cells and monocytes while not influencing T-cell migration.

Biological Activity

The biological activity determined by a chemotaxis bioassay using THP-1 human monocytic leukemia cells.The ED50 for this effect is 1.081 ng/mL, corresponding to a specific activity is 9.25×105 units/mg.

  • The biological activity determined by a chemotaxis bioassay using THP-1 human monocytic leukemia cells.The ED50 for this effect is 1.081 ng/mL, corresponding to a specific activity is 9.25×105 units/mg.
Species

Mouse

Source

E. coli

Tag

N-6*His

Accession

Q9WUQ5 (S23-E99)

Gene ID

57266  [NCBI]

Molecular Construction
N-term
6*His
Cxcl14 (S23-E99)
Accession # Q9WUQ5
C-term
Synonyms
Cxcl14; Bmac; Kec; Ks1; Mip2g; Scyb14C-X-C motif chemokine 14; B-cell and monocyte-activating chemokine; Chemokine BRAK; Kidney-expressed chemokine CXC; MIP-2G; Small-inducible cytokine B14
AA Sequence

SKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVIVTTKSMSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRVYEE

Molecular Weight

Approximately 13 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 300 mM arginine, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

CXCL14/BRAK Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CXCL14/BRAK Protein, Mouse (His)
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HY-P71887A
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