ICMT-IN-1
Based on 1 Customer Validation
ICMT-IN-1 (compound 75) is an inhibitor of ICMT (IC50=0.0013 μM). ICMT-IN-1 dose-dependently induces ICMT accumulation in the cytoplasm of HCT-116 cells and inhibits the proliferation of multiple cancer cell lines expressing K-Ras and N-Ras.
For research use only. We do not sell to patients.
- Purity: 98.43%
- CAS No.: 1313603-22-3
- Formula: C24H33NO2
- Molecular Weight:367.52
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 0.0013 μM (ICMT)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | GI50 |
0.3 μM
Compound: 75
|
Cytotoxicity against human CCRF-CEM cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human CCRF-CEM cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
|
[PMID: 21661760] |
| DU-145 | GI50 |
45 μM
Compound: 75
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Cytotoxicity against human DU145 cells expressing wild type Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human DU145 cells expressing wild type Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
|
[PMID: 21661760] |
| HCT-116 | GI50 |
20 μM
Compound: 75
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Cytotoxicity against human HCT116 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human HCT116 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
|
[PMID: 21661760] |
| HL-60 | GI50 |
3 μM
Compound: 75
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Cytotoxicity against human HL60 cells expressing N-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human HL60 cells expressing N-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
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[PMID: 21661760] |
| MIA PaCa-2 | GI50 |
19 μM
Compound: 75
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Cytotoxicity against human MIAPaCa2 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human MIAPaCa2 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
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[PMID: 21661760] |
| PC-3 | GI50 |
13 μM
Compound: 75
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Cytotoxicity against human PC3 cells expressing wild type Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human PC3 cells expressing wild type Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
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[PMID: 21661760] |
| SK-MEL-2 | GI50 |
>100 μM
Compound: 75
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Cytotoxicity against human SK-MEL-2 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human SK-MEL-2 cells expressing K-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
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[PMID: 21661760] |
| T-24 | GI50 |
12 μM
Compound: 75
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Cytotoxicity against human T24 cells expressing N-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
Cytotoxicity against human T24 cells expressing N-Ras assessed as growth inhibition after 7 days by luminescence-based ATPlite assay
|
[PMID: 21661760] |
Chemical Information
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CAS No. 1313603-22-3
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Appearance Solid
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Molecular Weight 367.52
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Formula C24H33NO2
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Color Brown to reddish brown
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SMILES
CC(C)(C1)OC(C)(C)CC1(C2=CC=CC=C2)CCNC3=CC(OC)=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (272.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7209 mL | 13.6047 mL | 27.2094 mL | 68.0235 mL |
| 5 mM | 0.5442 mL | 2.7209 mL | 5.4419 mL | 13.6047 mL | |
| 10 mM | 0.2721 mL | 1.3605 mL | 2.7209 mL | 6.8024 mL | |
| 15 mM | 0.1814 mL | 0.9070 mL | 1.8140 mL | 4.5349 mL | |
| 20 mM | 0.1360 mL | 0.6802 mL | 1.3605 mL | 3.4012 mL | |
| 25 mM | 0.1088 mL | 0.5442 mL | 1.0884 mL | 2.7209 mL | |
| 30 mM | 0.0907 mL | 0.4535 mL | 0.9070 mL | 2.2675 mL | |
| 40 mM | 0.0680 mL | 0.3401 mL | 0.6802 mL | 1.7006 mL | |
| 50 mM | 0.0544 mL | 0.2721 mL | 0.5442 mL | 1.3605 mL | |
| 60 mM | 0.0453 mL | 0.2267 mL | 0.4535 mL | 1.1337 mL | |
| 80 mM | 0.0340 mL | 0.1701 mL | 0.3401 mL | 0.8503 mL | |
| 100 mM | 0.0272 mL | 0.1360 mL | 0.2721 mL | 0.6802 mL |
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