Ro 67-7476
Based on 25 publication(s) in Google Scholar
Ro 67-7476 is a potent positive allosteric modulator of mGluR1 and potentiates glutamate-induced calcium release in HEK293 cells expressing rat mGluR1a with an EC50 of 60.1 nM. Ro 67-7476 is a potent P-ERK1/2 agonist and activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC50=163.3 nM).
For research use only. We do not sell to patients.
- Purity: 99.69%
- CAS No.: 298690-60-5
- Formula: C17H18FNO2S
- Molecular Weight:319.39
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Publications Citing Use of MedChemExpress (MCE) Ro 67-7476
More- Adv Sci (Weinh). 2025 Nov 21:e11943. [Abstract]
- Int J Biol Sci. 2022 Jan 1;18(2):473-490. [Abstract]
- J Transl Med. 2025 Nov 12;23(1):1271. [Abstract]
- Stem Cell Res Ther. 2024 Oct 8;15(1):350. [Abstract]
- Cell Death Discov. 2026 Jun 3. [Abstract]
- Cell Death Discov. 2024 Mar 26;10(1):153. [Abstract]
- Mol Med. 2024 Jun 15;30(1):89. [Abstract]
- Chem Biol Interact. 2024 Dec 1:404:111270. [Abstract]
- Pharmaceuticals (Basel). 2022 Aug 20;15(8):1027. [Abstract]
- Eur J Pharmacol. 2026 Feb 15:1015:178601. [Abstract]
- Int Immunopharmacol. 2025 Sep 23:162:114500. [Abstract]
- Int Immunopharmacol. 2025 May 23:159:114894. [Abstract]
- Int Immunopharmacol. 2025 May 10:158:114821. [Abstract]
- Int Immunopharmacol. 2024 Oct 31;143(Pt 3):113526. [Abstract]
- Int Immunopharmacol. 2022 Oct:111:109171. [Abstract]
- Mol Neurobiol. 2024 Feb;61(2):1175-1186. [Abstract]
- Transl Oncol. 2026 Feb:64:102658. [Abstract]
- J Funct Foods. 2024 Jan, 112, 105932.
- Cell Signal. 2025 Jun 17:111946. [Abstract]
- Andrology. 2025 Jun 16. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Jul 14;1869(7):159533. [Abstract]
- Cell Biol Int. 2025 Jan;49(1):68-78. [Abstract]
- Ren Fail. 2024 Dec;46(2):2431150. [Abstract]
- J Anim Sci. 2025 Jan 4:103:skaf266. [Abstract]
- Anticancer Drugs. 2026 Jan 14. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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Cell Migration/Invasion Assay
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Cell Migration/Invasion Assay
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Cell Imaging/Staining
Biological Activity
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mGluR1a 60.1 nM (EC50) |
In the Purkinje cells of rat cerebellar slices, Ro 67-7476 increases the amplitude of mGluR1 excitatory postsynaptic potentials (EPSCs) evoked by 2,3-dihydroxy-6-nitro-7-sulfamoylbenzoquionxaline, picrotoxin, or AP5[3]. Ro 67-7476 activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC50=163.3 nM). The EC50 value of full P-ERK1/2 activation for Ro 67-7476 are nearly identical to the EC50 for calcium mobilization potentiation[3].Ro 67-7476 increases basal cAMP production approximately by 8%. It potentiated threshold responses to glutamate in the cAMP accumulation assay, with an EC50 value of 17.7 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 298690-60-5
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Appearance Solid
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Molecular Weight 319.39
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Formula C17H18FNO2S
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Color White to yellow
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SMILES
O=S(N1[C@H](C2=CC=C(F)C=C2)CCC1)(C3=CC=C(C)C=C3)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (25)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Lipid-Driven OLR1/FOXM1/FGF19 Axis Orchestrates Crosstalk in an Epithelial-Fibroblast Positive Feedback Promoting Progesterone Resistance in Endometrial Cancer. [Abstract]2025 Nov 21:e11943. PMID: 41270216
Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e11943. [Abstract]
Cell viability was assessed using Ishikawa and AN3CA, with either a MEK inhibitor or an ERK agonist (Ro 67-7476; 48 h).
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Int J Biol Sci
ID09, A Newly-Designed Tubulin Inhibitor, Regulating the Proliferation, Migration, EMT Process and Apoptosis of Oral Squamous Cell Carcinoma. [Abstract]2022 Jan 1;18(2):473-490. PMID: 35002504
Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490. [Abstract]
Ro 67-7476 (1 μM for 5 min). Assess the activation level of the Ras-Erk signaling pathway in SCC-15 and Cal-27 cells after ID09 treatment.
Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490. [Abstract]
After 24 and 48 hours of ID09 treatment, the wound healing rate of SCC-15 cells decreased, while treatment with Ro 67-7476 restored wound healing.
Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490. [Abstract]
The figure above shows the results of a clonogenic assay of OSCC cells treated with ID09 and/or Ro 67-7476.
Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490. [Abstract]
Ro 67-7476 can rescue ID09-mediated apoptosis in SCC-15 cells. The survival, apoptosis, and necrosis of cultured OSCC cells can be visually visualized using the Hoechst 33342-PI double staining method.
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J Transl Med
Retinal ALKBH5 inhibition induces myopia protection through selective regulation of ERK1/2 signaling. [Abstract]2025 Nov 12;23(1):1271. PMID: 41225555 -
Stem Cell Res Ther
SMYD1 modulates the proliferation of multipotent cardiac progenitor cells derived from human pluripotent stem cells during myocardial differentiation through GSK3β/β-catenin&ERK signaling. [Abstract]2024 Oct 8;15(1):350. PMID: 39380045 -
Cell Death Discov
Apatinib inhibits synovial sarcoma progression and angiogenesis via VEGFR2-mediated AKT/FOXO3A and ERK1/2/FOXM1 signaling pathways. [Abstract]2026 Jun 3. PMID: 42236679 -
Cell Death Discov
Preso enhances mGluR1-mediated excitotoxicity by modulating the phosphorylation of mGluR1-Homer1 complex and facilitating an ER stress after traumatic brain injury. [Abstract]2024 Mar 26;10(1):153. PMID: 38531909 -
Mol Med
Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction. [Abstract]2024 Jun 15;30(1):89. PMID: 38879488 -
Chem Biol Interact
Oxymatrine inhibits migration and invasion of esophageal squamous cell carcinoma cell lines via the MEK1/ERK/β-catenin pathway. [Abstract]2024 Dec 1:404:111270. PMID: 39419199 -
Pharmaceuticals (Basel)
2022 Aug 20;15(8):1027. PMID: 36015175 -
Eur J Pharmacol
Bufotalin targets COL1A1 to suppress non-small cell lung cancer growth and remodel the tumor microenvironment via dual inhibition of PI3K/AKT/mTOR and MAPK pathways. [Abstract]2026 Feb 15:1015:178601. PMID: 41581717 -
Int Immunopharmacol
FN14 promotes ventilator-induced lung injury by regulating pyroptosis via the ANXA2/ERK1/2 axis. [Abstract]2025 Sep 23:162:114500. PMID: 40744784 -
Int Immunopharmacol
Isorhapontigenin suppresses inflammation, proliferation and aggressiveness of rheumatoid arthritis fibroblast-like synoviocytes by targeting farnesyl diphosphate synthase. [Abstract]2025 May 23:159:114894. PMID: 40412131 -
Int Immunopharmacol
Fucosterol ameliorates depressive-like behaviours by suppressing microglial activation and neuroinflammation via inhibition the MAPK/ERK1/2 signaling pathway. [Abstract]2025 May 10:158:114821. PMID: 40349406 -
Int Immunopharmacol
CD83 mediates the inhibitory effect of the S1PR1 agonist CYM5442 on LPS-induced M1 polarization of macrophages through the ERK-STAT-1 signaling pathway. [Abstract]2024 Oct 31;143(Pt 3):113526. PMID: 39486189 -
Int Immunopharmacol
Gondoic acid alleviates LPS‑induced Kupffer cells inflammation by inhibiting ROS production and PKCθ/ERK/STAT3 signaling pathway. [Abstract]2022 Oct:111:109171. PMID: 35998508 -
Mol Neurobiol
TGN-020 Alleviate Inflammation and Apoptosis After Cerebral Ischemia-Reperfusion Injury in Mice Through Glymphatic and ERK1/2 Signaling Pathway. [Abstract]2024 Feb;61(2):1175-1186. PMID: 37695472 -
Transl Oncol
Zeaxanthin targets TOP2A to regulate autophagy and suppress lung cancer progression via the MAPK/ERK pathway. [Abstract]2026 Feb:64:102658. PMID: 41496415 -
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Cell Signal
The role of FAK signaling in early placental development and trophoblast lineage specification in human pregnancy. [Abstract]2025 Jun 17:111946. PMID: 40553964 -
Andrology
Expression of IL-33 in rodent testes and its role in Leydig cell steroidogenesis and aging. [Abstract]2025 Jun 16. PMID: 40521800 -
Biochim Biophys Acta Mol Cell Biol Lipids
Ilexgenin A inhibits lipid accumulation in macrophages and reduces the progression of atherosclerosis through PTPN2/ERK1/2/ABCA1 signalling pathway. [Abstract]2024 Jul 14;1869(7):159533. PMID: 39009241 -
Cell Biol Int
Flurbiprofen axetil is involved in basal-like breast cancer metastasis via suppressing the MEK/ERK signaling pathway. [Abstract]2025 Jan;49(1):68-78. PMID: 39364685 -
Ren Fail
TRPC6 knockdown-mediated ERK1/2 inactivation alleviates podocyte injury in minimal change disease via upregulating Lon peptidase 1. [Abstract]2024 Dec;46(2):2431150. PMID: 39566913 -
J Anim Sci
Roles of JNK, ERK, and PI3K/AKT signaling pathways in zinc-mediated alleviation of thermal stress-induced damage to the integrity and barrier function of primary cultured chick jejunal epithelial cells. [Abstract]2025 Jan 4:103:skaf266. PMID: 40795135 -
Anticancer Drugs
APG-1252 enhances the anticancer role of paclitaxel in non-small-cell lung cancer cells by suppressing the extracellular regulated protein kinases/myeloid cell leukemia 1 pathway. [Abstract]2026 Jan 14. PMID: 41608859
Solvent & Solubility
DMSO : ≥ 40 mg/mL (125.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. F Knoflach, et al. Positive allosteric modulators of metabotropic glutamate 1 receptor: characterization, mechanism of action, and binding site. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13402-7 [Content Brief]
[2]. Kamondanai Hemstapat, et al. A novel class of positive allosteric modulators of metabotropic glutamate receptor subtype 1 interact with a site distinct from that of negative allosteric modulators. Mol Pharmacol. 2006 Aug;70(2):616-26. [Content Brief]
[3]. Douglas J Sheffler, et al. Allosteric potentiators of metabotropic glutamate receptor subtype 1a differentially modulate independent signaling pathways in baby hamster kidney cells. Neuropharmacology. 2008 Sep;55(4):419-27 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1310 mL | 15.6548 mL | 31.3097 mL | 78.2742 mL |
| 5 mM | 0.6262 mL | 3.1310 mL | 6.2619 mL | 15.6548 mL | |
| 10 mM | 0.3131 mL | 1.5655 mL | 3.1310 mL | 7.8274 mL | |
| 15 mM | 0.2087 mL | 1.0437 mL | 2.0873 mL | 5.2183 mL | |
| 20 mM | 0.1565 mL | 0.7827 mL | 1.5655 mL | 3.9137 mL | |
| 25 mM | 0.1252 mL | 0.6262 mL | 1.2524 mL | 3.1310 mL | |
| 30 mM | 0.1044 mL | 0.5218 mL | 1.0437 mL | 2.6091 mL | |
| 40 mM | 0.0783 mL | 0.3914 mL | 0.7827 mL | 1.9569 mL | |
| 50 mM | 0.0626 mL | 0.3131 mL | 0.6262 mL | 1.5655 mL | |
| 60 mM | 0.0522 mL | 0.2609 mL | 0.5218 mL | 1.3046 mL | |
| 80 mM | 0.0391 mL | 0.1957 mL | 0.3914 mL | 0.9784 mL | |
| 100 mM | 0.0313 mL | 0.1565 mL | 0.3131 mL | 0.7827 mL |
Ro 67-7476 Related Classifications
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