2. GPCR/G Protein Neuronal Signaling
  3. mGluR
  4. Ro 67-7476

Ro 67-7476 is a potent positive allosteric modulator of mGluR1 and potentiates glutamate-induced calcium release in HEK293 cells expressing rat mGluR1a with an EC50 of 60.1 nM. Ro 67-7476 is a potent P-ERK1/2 agonist and activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC50=163.3 nM).

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CAS No. : 298690-60-5

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Customer Review

Based on 23 publication(s) in Google Scholar

Other Forms of Ro 67-7476:

Ro 67-7476 Related Antibodies

Top Publications Citing Use of Products

    Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e11943.  [Abstract]

    Cell viability was assessed using Ishikawa and AN3CA, with either a MEK inhibitor or an ERK agonist (Ro 67-7476; 48 h).

    Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490.  [Abstract]

    Ro 67-7476 (1 μM for 5 min). Assess the activation level of the Ras-Erk signaling pathway in SCC-15 and Cal-27 cells after ID09 treatment.

    Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490.  [Abstract]

    After 24 and 48 hours of ID09 treatment, the wound healing rate of SCC-15 cells decreased, while treatment with Ro 67-7476 restored wound healing.

    Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490.  [Abstract]

    The figure above shows the results of a clonogenic assay of OSCC cells treated with ID09 and/or Ro 67-7476.

    Ro 67-7476 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jan 1;18(2):473-490.  [Abstract]

    Ro 67-7476 can rescue ID09-mediated apoptosis in SCC-15 cells. The survival, apoptosis, and necrosis of cultured OSCC cells can be visually visualized using the Hoechst 33342-PI double staining method.

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    Description

    Ro 67-7476 is a potent positive allosteric modulator of mGluR1 and potentiates glutamate-induced calcium release in HEK293 cells expressing rat mGluR1a with an EC50 of 60.1 nM[1][2]. Ro 67-7476 is a potent P-ERK1/2 agonist and activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC50=163.3 nM)[3].

    IC50 & Target[1]

    mGluR1a

    60.1 nM (EC50)

    In Vitro

    In the Purkinje cells of rat cerebellar slices, Ro 67-7476 increases the amplitude of mGluR1 excitatory postsynaptic potentials (EPSCs) evoked by 2,3-dihydroxy-6-nitro-7-sulfamoylbenzoquionxaline, picrotoxin, or AP5[3].
    Ro 67-7476 activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC50=163.3 nM). The EC50 value of full P-ERK1/2 activation for Ro 67-7476 are nearly identical to the EC50 for calcium mobilization potentiation[3].
    Ro 67-7476 increases basal cAMP production approximately by 8%. It potentiated threshold responses to glutamate in the cAMP accumulation assay, with an EC50 value of 17.7 μM[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ro 67-7476 Related Antibodies
    Molecular Weight

    319.39

    Formula

    C17H18FNO2S
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=S(N1[C@H](C2=CC=C(F)C=C2)CCC1)(C3=CC=C(C)C=C3)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 40 mg/mL (125.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1310 mL 15.6548 mL 31.3097 mL
    5 mM 0.6262 mL 3.1310 mL 6.2619 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1310 mL 15.6548 mL 31.3097 mL 78.2742 mL
    5 mM 0.6262 mL 3.1310 mL 6.2619 mL 15.6548 mL
    10 mM 0.3131 mL 1.5655 mL 3.1310 mL 7.8274 mL
    15 mM 0.2087 mL 1.0437 mL 2.0873 mL 5.2183 mL
    20 mM 0.1565 mL 0.7827 mL 1.5655 mL 3.9137 mL
    25 mM 0.1252 mL 0.6262 mL 1.2524 mL 3.1310 mL
    30 mM 0.1044 mL 0.5218 mL 1.0437 mL 2.6091 mL
    40 mM 0.0783 mL 0.3914 mL 0.7827 mL 1.9569 mL
    50 mM 0.0626 mL 0.3131 mL 0.6262 mL 1.5655 mL
    60 mM 0.0522 mL 0.2609 mL 0.5218 mL 1.3046 mL
    80 mM 0.0391 mL 0.1957 mL 0.3914 mL 0.9784 mL
    100 mM 0.0313 mL 0.1565 mL 0.3131 mL 0.7827 mL
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    Ro 67-7476 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ro 67-7476298690-60-5mGluRMetabotropic glutamate receptorstraumatic injurycAMP accumulationexcitatory postsynaptic potentialsmGluR1neuronal cultureInhibitorinhibitorinhibit

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