Plamotamab
Based on 1 Customer Validation
Plamotamab (XmAb-13676) is a human bispecific antibody (bsAb) that binds CD3 and CD20. Plamotamab recruits cytotoxic T cells to kill CD20+ expressing tumor cells. Plamotamab induces a mild hematologic reaction (MR), and results in tumor regression in vivo.
For research use only. We do not sell to patients.
- Purity: 99.12%
- CAS No.: 2138442-31-4
- Molecular Weight:124.88 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
half-IGG1-kappa/(scFv-heavy-lambda)-h-CH2-CH3
Human
CD3E & MS4A1
Plamotamab induces the killing of endogenous B cells and activation of endogenous CD8+ T cells within human PBMCs in vitro, with an EC50 value of 0.113 nM[2].
Plamotamab induces T cell-mediated killing in Raji tumor cells in vitro, with an EC50 value of 0.144 nM[2].
Plamotamab binds to CD3δ/ε, CD3+ T cells, CD4+ T cells, CD8+ T cells, B cells, Raji cells, with EC50s of 2.1 nM, 11.78 nM, 10.12 nM, 200.3 nM, and 134.8 nM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Plamotamab (0.3 mg/kg; i.p.) leads to cytokine release in cynomolgus monkeys, indicating a safety to be improved[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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half-IGG1-kappa/(scFv-heavy-lambda)-h-CH2-CH3
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD3E (red) with Plamotamab (HY-P99776). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.
Chemical Information
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CAS No. 2138442-31-4
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Appearance Liquid
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Molecular Weight 124.88 kDa
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Color Colorless to light yellow
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SMILES
[plamotamab]
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Synonyms
XmAb-13676
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (258 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)