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Hepcidin/HAMP Protein, Mouse (GST)

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The hepcidin/HAMP protein is a liver-produced hormone that is a major regulator of iron absorption and distribution. It promotes endocytosis and degradation of SLC40A1, retaining iron within cells and reducing plasma iron flux. Hepcidin/HAMP Protein, Mouse (GST) is the recombinant mouse-derived Hepcidin/HAMP protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Hepcidin/HAMP Protein, Mouse (GST)
  • Experimental Validation Results for Hepcidin/HAMP Protein, Mouse (GST)
  • Hepcidin/HAMP Protein, Mouse (GST) Product Image
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The hepcidin/HAMP protein is a liver-produced hormone that is a major regulator of iron absorption and distribution. It promotes endocytosis and degradation of SLC40A1, retaining iron within cells and reducing plasma iron flux. Hepcidin/HAMP Protein, Mouse (GST) is the recombinant mouse-derived Hepcidin/HAMP protein, expressed by E. coli , with N-GST labeled tag.

Background

Hepcidin, a liver-produced hormone, stands as the principal circulating regulator governing the absorption and distribution of iron throughout the body. Its regulatory mechanism involves the promotion of endocytosis and degradation of SLC40A1, resulting in the retention of iron within iron-exporting cells and a consequential reduction in iron flow into the plasma. Hepcidin orchestrates vital iron fluxes, including the absorption of dietary iron in the intestine, the recycling of iron by macrophages through phagocytosis of aging erythrocytes and other cells, and the mobilization of stored iron from hepatocytes. Its interaction with SLC40A1 triggers rapid ubiquitination of the latter, underscoring hepcidin's pivotal role in maintaining iron homeostasis.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized HAMP at 10μg/mL (100μL/well) can bind Anti-HAMP antibody. The ED50 for this effect is 1.303 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized HAMP at 10 μg/mL (100 μL/well) can bind Anti-HAMP antibody. The ED50 for this effect is 1.303 μg/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-GST
  • Accession

    Q9EQ21 (D59-T83)

  • Gene ID
    84506  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • Hepcidin (D59-T83)
      Accession # Q9EQ21
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    HAMP; Liver-Expressed Antimicrobial Peptide 1; Hepcidin Antimicrobial Peptide; Putative Liver Tumor Regressor; LEAP1; Hepcidin; HEPC; PLTR; LEAP-1; Hepcidin Preproprotein; HFE2B

  • AA Sequence

    DTNFPICIFCCKCCNNSQCGICCKT

  • Molecular Weight

    Approximately 29 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized HAMP at 10 μg/mL (100 μL/well) can bind Anti-HAMP antibody. The ED50 for this effect is 1.303 μg/mL.

  • Hepcidin/HAMP Protein, Mouse (GST) Product Image
    1/16
  • Experimental Validation Results for Hepcidin/HAMP Protein, Mouse (GST)
  • Experimental Validation Results for Hepcidin/HAMP Protein, Mouse (GST)
  • Hepcidin/HAMP Protein, Mouse (GST) Product Image