Apelin-17(human, bovine)
Based on 1 Customer Validation
Apelin-17(human, bovine) is an endogenous orphan G protein-coupled receptor APJ agonist. Apelin-17(human, bovine) binds to human APJ receptors expressed in HEK 293 cells (pIC50=9.02). Apelin-17(human, bovine) inhibits the entry of some HIV-1 and HIV-2 into the NP2/CD4 cells expressing APJ.
For research use only. We do not sell to patients.
- Purity: 98.71%
- CAS No.: 217082-57-0
- Formula: C96H156N34O20S
- Molecular Weight:2138.55
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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HIV-1 |
HIV-2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
1.4 x 10-3 μM
Compound: 2, K17F
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Agonist activity at rat apelin receptor expressed in CHO cell membranes coexpressing EGFP assessed as inhibition of forskolin-induced cAMP production after 30 mins by fluorescence analysis
Agonist activity at rat apelin receptor expressed in CHO cell membranes coexpressing EGFP assessed as inhibition of forskolin-induced cAMP production after 30 mins by fluorescence analysis
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[PMID: 24625069] |
| CHO | IC50 |
0.025 nM
Compound: 1
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Displacement of [125I]-(Glp1,Nle11,Try14)apelin-13 from recombinant rat EGFP-fused apelin receptor expressed in CHO cell membrane measured after 3 hrs
Displacement of [125I]-(Glp1,Nle11,Try14)apelin-13 from recombinant rat EGFP-fused apelin receptor expressed in CHO cell membrane measured after 3 hrs
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[PMID: 30690406] |
Chemical Information
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CAS No. 217082-57-0
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Appearance Solid
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Molecular Weight 2138.55
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Formula C96H156N34O20S
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Color White to off-white
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Sequence
Lys-Phe-Arg-Arg-Gln-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe
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Sequence Shortening
KFRRQRPRLSHKGPMPF
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 100 mg/mL (46.76 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Tatemoto K, Hosoya M, Habata Y, et al. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochem Biophys Res Commun. 1998;251(2):471-476. [Content Brief]
[2]. Medhurst AD, Jennings CA, Robbins MJ, et al. Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin. J Neurochem. 2003;84(5):1162-1172. [Content Brief]
[3]. Zou MX, et al. Apelin peptides block the entry of human immunodeficiency virus (HIV). FEBS Lett. 2000;473(1):15-18. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4676 mL | 2.3380 mL | 4.6761 mL | 11.6902 mL |
| 5 mM | 0.0935 mL | 0.4676 mL | 0.9352 mL | 2.3380 mL | |
| 10 mM | 0.0468 mL | 0.2338 mL | 0.4676 mL | 1.1690 mL | |
| 15 mM | 0.0312 mL | 0.1559 mL | 0.3117 mL | 0.7793 mL | |
| 20 mM | 0.0234 mL | 0.1169 mL | 0.2338 mL | 0.5845 mL | |
| 25 mM | 0.0187 mL | 0.0935 mL | 0.1870 mL | 0.4676 mL | |
| 30 mM | 0.0156 mL | 0.0779 mL | 0.1559 mL | 0.3897 mL | |
| 40 mM | 0.0117 mL | 0.0585 mL | 0.1169 mL | 0.2923 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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