Ferrichrome Iron-free
Based on 1 Customer Validation
Ferrichrome Iron-free (Desferrichrome; DFC; N-Desferriferrichrome) is the ligand form of iron-loaded Ferrichrome when it is not bound to Fe3+. Ferrichrome Iron-free induces apoptosis in gastric cancer cells by activating the JNK-DDIT3 apoptotic axis. As an iron chelator, Ferrichrome Iron-free enables dual-modal imaging of HER2 via conjugation with SiR and Trastuzumab (HY-P9907). Ferrichrome Iron-free can be used in the research of gastric cancer and HER2-overexpressing cancers.
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- Pureté: 98.5%
- CAS No.: 34787-28-5
- Formule: C27H45N9O12
- Masse moléculaire:687.70
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Stockage:
Stored under nitrogen, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Iron-free Ferrichrome (1-100 µg/mL; 72-120 h) inhibits the growth of MKN-7, MKN-45, MKN-74 and NUGC-4 gastric cancer cells in a dose-dependent manner, with the strongest inhibitory activity against MKN-7 (IC50 = 0.86 µg/mL) and MKN-45 (IC50 = 0.87 µg/mL) cells after 72 h of treatment[1].
Iron-free Ferrichrome (1-100 µg/mL; 72 h, 120 h, 3 h) induces the expression of apoptosis-related proteins (cleaved PARP, cleaved caspase-9, cleaved caspase-8) in MKN-7, MKN-45, MKN-74 and NUGC-4 gastric cancer cells, and upregulates the phosphorylation level of JNK in MKN-45 gastric cancer cells[1].
Iron-free Ferrichrome (1 µg/mL; 72 h) increases the population of apoptotic cells in the G1 subphase in MKN-74 gastric cancer cells, but does not alter the cell cycle distribution of MKN-7, MKN-45 or NUGC-4 gastric cancer cells[1].
Iron-free Ferrichrome (0.2-1 µg/mL; 48 h) dose-dependently induces DDIT3 mRNA expression in MKN-45 gastric cancer cells, and significantly induces DDIT3 mRNA expression in MKN-7 gastric cancer cells after 48 h of treatment, but does not alter DDIT3 expression in MKN-74 or NUGC-4 gastric cancer cells[1].
Iron-free Ferrichrome (1-100 µg/mL; 24 h) reduces intracellular iron levels in MKN-7, MKN-45, MKN-74 and NUGC-4 gastric cancer cells to similar levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MKN-7, MKN-45, MKN-74, NUGC-4
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Concentration:1 µg/mL, 10 µg/mL, 100 µg/mL
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Incubation Time:24 h, 72 h, 120 h
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Result:Exerted dose-dependent growth-suppressive effects on all four gastric cancer cell lines after 72-120 h of treatment.
Demonstrated stronger potency in MKN-7 and MKN-45 cells, with 72 h IC50 values of 0.86 µg/mL and 0.87 µg/mL, respectively.
Suppressed growth significantly only at the highest concentration (100 µg/mL) in MKN-74 and NUGC-4 cells, with 72 h IC50 values of 107 µg/mL and 13 µg/mL, respectively.
Associated all significant growth suppression with p < 0.05 by Student’s t test.
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Cell Line:MKN-7, MKN-45, MKN-74, NUGC-4
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Concentration:1 µg/mL, 10 µg/mL, 100 µg/mL (apoptosis-related proteins); 1 µg/mL (JNK phosphorylation, MKN-45)
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Incubation Time:72 h (MKN-7, MKN-45), 120 h (MKN-74, NUGC-4) (apoptosis-related proteins); 3 h (JNK phosphorylation, MKN-45)
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Result:Increased the expression of cleaved poly (adenosine diphosphate-ribose) polymerase (PARP) and cleaved caspase-9 in all four cell lines at 100 µg/mL (p < 0.05).
Induced cleaved PARP expression in MKN-45 and MKN-74 cells even at 1 µg/mL.
Detected cleaved caspase-8 in MKN-7, MKN-74, and NUGC-4 cells, but not in MKN-45 cells.
Upregulated phosphorylation of stress-activated protein kinase (SAPK)/JNK in MKN-45 cells at 1 µg/mL after 3 h of treatment (p < 0.05).
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Cell Line:MKN-7, MKN-45, MKN-74, NUGC-4
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Concentration:200 ng/mL, 1 µg/mL
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Incubation Time:48 h
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Result:Significantly induced DDIT3 mRNA expression in MKN-7 and MKN-45 cells at 1 µg/mL.
Induced DDIT3 mRNA expression in a dose-dependent manner in MKN-45 cells, with significant increases at both 200 ng/mL and 1 µg/mL.
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Cell Line:MKN-7, MKN-45, MKN-74, NUGC-4
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Concentration:1.0 µg/mL
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Incubation Time:72 h
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Result:Increased the sub-G1 peak (a marker of apoptotic cell death) in MKN-74 cells.
Did not significantly alter the cell cycle profile or sub-G1 peak in MKN-7, MKN-45, or NUGC-4 cells.
89Zr-SiR-LDFC-trastuzumab, a conjugate integrated with Ferrichrome Iron-free (1.1-1.3 MBq; intravenous injection; single dose), achieves a tumor uptake of 16.9 %ID/g at 96 hours post-injection in HER2+ SKOV-3 xenograft mice, and enables successful fluorescence-guided tumor resection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nu/nu nude mice (male, 6 weeks of age)[1]
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Dosage:100 µg
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Administration:i.p.; daily
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Result:Reduced tumor volume ratio (tumor size/day 2 size) significantly at days 17, 19, and 21 compared to controls.
Showed no marked differences in hemoglobin levels or mean corpuscular volume (MCV) compared to controls.
Lost tumor-suppressive effects when ferrichrome was iron-chelated.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 34787-28-5
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Appearance Solid
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Masse moléculaire 687.70
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Formule C27H45N9O12
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Color White to off-white
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Synonyms
Desferrichrome; DFC; N-Desferriferrichrome
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Stored under nitrogen, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvant et solubilité
H2O : 10 mg/mL (14.54 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (286 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Ijiri M, et al. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2017 Jun;39(6):1010428317711311. [Content Brief]
[2]. Ahn SH, et al. Linear Desferrichrome-Linked Silicon-Rhodamine Antibody Conjugate Enables Targeted Multimodal Imaging of HER2 in Vitro and in Vivo. Molecular pharmaceutics. 2019 Mar 04;16(3):1412-1420. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.4541 mL | 7.2706 mL | 14.5412 mL | 36.3531 mL |
| 5 mM | 0.2908 mL | 1.4541 mL | 2.9082 mL | 7.2706 mL | |
| 10 mM | 0.1454 mL | 0.7271 mL | 1.4541 mL | 3.6353 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ferrichrome Iron-free Related Classifications
HY-P1997 Related Classifications
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