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Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)

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PLET1 protein orchestrates leading keratinocyte migration and enhances cellular adhesion to matrix proteins, crucial for wound healing. Its role extends to promoting efficient wound repair, emphasizing its significance in tissue regeneration. Additionally, PLET1 may influence trichilemmal differentiation in the hair follicle, indicating a broader impact on skin biology and homeostasis. Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Placenta-expressed transcript 1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)
  • Experimental Validation Results for Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)
  • Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

PLET1 protein orchestrates leading keratinocyte migration and enhances cellular adhesion to matrix proteins, crucial for wound healing. Its role extends to promoting efficient wound repair, emphasizing its significance in tissue regeneration. Additionally, PLET1 may influence trichilemmal differentiation in the hair follicle, indicating a broader impact on skin biology and homeostasis. Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Placenta-expressed transcript 1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Background

Placenta-expressed transcript 1 (PLET1) protein plays a pivotal role in orchestrating leading keratinocyte migration and facilitating cellular adhesion to matrix proteins, thereby contributing to the intricate process of wound healing. Its involvement extends to promoting efficient wound repair, underscoring its significance in tissue regeneration. Furthermore, PLET1 may exert its influence during trichilemmal differentiation within the context of the hair follicle, suggesting a broader impact on skin biology and homeostasis.

Verified Bioactivity

Immobilized Mouse Placenta-expressed transcript 1 at 1 μg/mL (100 μL/well) can bind Anti- Placenta-expressed transcript 1. The ED50 for this effect is 0.03 μg/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-His;N-SUMO
  • Accession

    Q8VEN2-1 (S28-S218)

  • Gene ID
    76509  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • PLET1 (S28-S218)
      Accession # Q8VEN2
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PLET1; Placenta-Expressed Transcript 1 Protein; Prev. C11orf34; Placenta-Expressed Transcript 1; Placenta Expressed Transcript 1; Chromosome 11 Open Reading Frame 34

  • AA Sequence

    SDNGSCVVLDNIYTSDILEISTMANVSGGDVTYTVTVPVNDSVSAVILKAVKEDDSPVGTWSGTYEKCNDSSVYYNLTSQSQSVFQTNWTVPTSEDVTKVNLQVLIVVNRTASKSSVKMEQVQPSASTPIPESSETSQTINTTPTVNTAKTTAKDTANTTAVTTANTTANTTAVTTAKTTAKSLAIRTLGS

  • Molecular Weight

    Approximately 43 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 6% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Mouse Placenta-expressed transcript 1 at 1 μg/mL (100 μL/well) can bind Anti- Placenta-expressed transcript 1. The ED50 for this effect is 0.03 μg/mL.

  • Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) Product Image
    1/16
  • Experimental Validation Results for Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)
  • Experimental Validation Results for Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)
  • Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) Product Image