Lewis X trisaccharide
Based on 1 Customer Validation
Lewis X trisaccharide (Lewis X, Lex) is a potent TH2 regulator, antagonizes LPS-induced IL-12 immune expression. Lewis X trisaccharide is a human histo-blood group antigen, plays an key role in cell-cell adhesion, and servers as a tumor marker. Lewis X trisaccharide is highly expressed in the outer membrane of the parasite, can be used for the immunology research of schistosomiasis.
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- Pureté: 98.0%
- CAS No.: 71208-06-5
- Formule: C20H35NO15
- Masse moléculaire:529.49
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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Schistosome |
Lewis X trisaccharide-BSA (25 μg/mL; 30 min; before LPS stimulation of 10 ng/mL for 4 h) IL-12p40 and suppresses IL-12p70 protein expression induced by Lipopolysaccharide (LPS, HY-D1056)[1].
Lewis X trisaccharide (2 μM; 30 min; before LPS stimulation of 10 ng/mL for 2 h) decreases nuclear NF-κB concentration in mice bone marrow derived dendritic cells (BDDCs)[1].
Lewis X trisaccharide-BSA (25 μg/mL; 48 h) or Lewis X trisaccharide (5 μg/mL; 48 h) plus ovalbumin (OVA, 25 μg/mL) increases cytokines (IL-4, IL-13, and INF-γ) level in mice splenocytes[1].
Lewis X trisaccharide-containing glycoconjugates stimulates B cells to proliferate and to produce factors that down-regulates the TH1 immune response and up-regulates the TH2 immune response[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice (BALB/c, IL-12 deficient on a BALB/c background, TLR4-defective C3H/hej, or TLR4-wild type C3H/HeOuj mice) (6-8 weeks old)[1]
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Dosage:5 μg, combinded with 50 μg ovalbumin
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Administration:Subcutaneous injection; once a week for 2 weeks
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Result:In C3H mice, coupled with BSA (LeX-BSA) and elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic TH2 cytokines when compared with those seen in BSA-sensitized mice.
In BALB/c mice, induced by ovalbumin, significantly increased levels of specific IgE, resulted IgG2a antibodies concomitant with reduced levels of serum IL-12p70.
In IL-12-deficient BALB/c mice, attenuated the downward trend of the above indicators.
Chemical Information
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CAS No. 71208-06-5
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Appearance Solid
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Masse moléculaire 529.49
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Formule C20H35NO15
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Color White to off-white
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SMILES
OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@H]1O[C@H]([C@H](O)CO)[C@@H]([C@H](C=O)NC(C)=O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
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Synonyms
Lewis X
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Structure Classification
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Initial Source
human
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
H2O : ≥ 100 mg/mL (188.86 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Hsu SC, et al. Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice. J Allergy Clin Immunol. 2007 Jun;119(6):1522-8. [Content Brief]
[2]. van Roon AM, et al. Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen. Structure. 2004 Jul;12(7):1227-36. [Content Brief]
[3]. Topin J, et al. The Hidden Conformation of Lewis x, a Human Histo-Blood Group Antigen, Is a Determinant for Recognition by Pathogen Lectins. ACS Chem Biol. 2016 Jul 15;11(7):2011-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8886 mL | 9.4430 mL | 18.8861 mL | 47.2152 mL |
| 5 mM | 0.3777 mL | 1.8886 mL | 3.7772 mL | 9.4430 mL | |
| 10 mM | 0.1889 mL | 0.9443 mL | 1.8886 mL | 4.7215 mL | |
| 15 mM | 0.1259 mL | 0.6295 mL | 1.2591 mL | 3.1477 mL | |
| 20 mM | 0.0944 mL | 0.4722 mL | 0.9443 mL | 2.3608 mL | |
| 25 mM | 0.0755 mL | 0.3777 mL | 0.7554 mL | 1.8886 mL | |
| 30 mM | 0.0630 mL | 0.3148 mL | 0.6295 mL | 1.5738 mL | |
| 40 mM | 0.0472 mL | 0.2361 mL | 0.4722 mL | 1.1804 mL | |
| 50 mM | 0.0378 mL | 0.1889 mL | 0.3777 mL | 0.9443 mL | |
| 60 mM | 0.0315 mL | 0.1574 mL | 0.3148 mL | 0.7869 mL | |
| 80 mM | 0.0236 mL | 0.1180 mL | 0.2361 mL | 0.5902 mL | |
| 100 mM | 0.0189 mL | 0.0944 mL | 0.1889 mL | 0.4722 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Lewis X trisaccharide Related Classifications
HY-N10534 Related Classifications
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