Lewis X trisaccharide
Based on 1 Customer Validation
Lewis X trisaccharide (Lewis X, Lex) is a potent TH2 regulator, antagonizes LPS-induced IL-12 immune expression. Lewis X trisaccharide is a human histo-blood group antigen, plays an key role in cell-cell adhesion, and servers as a tumor marker. Lewis X trisaccharide is highly expressed in the outer membrane of the parasite, can be used for the immunology research of schistosomiasis.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.0%
- CAS. Nr.: 71208-06-5
- Formel: C20H35NO15
- Molecular Weight:529.49
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
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Schistosome |
In Vitro
Lewis X trisaccharide-BSA (25 μg/mL; 30 min; before LPS stimulation of 10 ng/mL for 4 h) IL-12p40 and suppresses IL-12p70 protein expression induced by Lipopolysaccharide (LPS, HY-D1056)[1].
Lewis X trisaccharide (2 μM; 30 min; before LPS stimulation of 10 ng/mL for 2 h) decreases nuclear NF-κB concentration in mice bone marrow derived dendritic cells (BDDCs)[1].
Lewis X trisaccharide-BSA (25 μg/mL; 48 h) or Lewis X trisaccharide (5 μg/mL; 48 h) plus ovalbumin (OVA, 25 μg/mL) increases cytokines (IL-4, IL-13, and INF-γ) level in mice splenocytes[1].
Lewis X trisaccharide-containing glycoconjugates stimulates B cells to proliferate and to produce factors that down-regulates the TH1 immune response and up-regulates the TH2 immune response[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice (BALB/c, IL-12 deficient on a BALB/c background, TLR4-defective C3H/hej, or TLR4-wild type C3H/HeOuj mice) (6-8 weeks old)[1]
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Dosage:5 μg, combinded with 50 μg ovalbumin
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Administration:Subcutaneous injection; once a week for 2 weeks
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Result:In C3H mice, coupled with BSA (LeX-BSA) and elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic TH2 cytokines when compared with those seen in BSA-sensitized mice.
In BALB/c mice, induced by ovalbumin, significantly increased levels of specific IgE, resulted IgG2a antibodies concomitant with reduced levels of serum IL-12p70.
In IL-12-deficient BALB/c mice, attenuated the downward trend of the above indicators.
Chemical Information
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CAS. Nr. 71208-06-5
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Appearance Solid
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Molecular Weight 529.49
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Formel C20H35NO15
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Color White to off-white
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SMILES
OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@H]1O[C@H]([C@H](O)CO)[C@@H]([C@H](C=O)NC(C)=O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
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Synonyms
Lewis X
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Structure Classification
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Initial Source
human
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
H2O : ≥ 100 mg/mL (188.86 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Naïve CD4+ T-cell subset differentiation/polarization
Naïve CD4+ T-cell subset differentiation/polarization is an in vitro assay in which purified naïve CD4+ T cells are activated through TCR and CD28 costimulation and cultured with defined cytokines and neutralizing antibodies to generate Th0, Th1, Th2, Th17, or induced Treg-like populations. Differentiation is detected by subset-associated cytokines and transcription factors: IFN-γ/T-bet for Th1, IL-4/GATA3 for Th2, IL-17A/RORγt for Th17, and Foxp3 for induced Treg cells. The assay readout is usually generated by intracellular cytokine staining after restimulation, transcription-factor staining by flow cytometry, ELISA of secreted cytokines, or gene-expression analysis. The result reflects cytokine-directed lineage commitment or polarization rather than antigen-specific immune protection by itself.
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Reinheit & Dokumentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Hsu SC, et al. Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice. J Allergy Clin Immunol. 2007 Jun;119(6):1522-8. [Content Brief]
[2]. van Roon AM, et al. Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen. Structure. 2004 Jul;12(7):1227-36. [Content Brief]
[3]. Topin J, et al. The Hidden Conformation of Lewis x, a Human Histo-Blood Group Antigen, Is a Determinant for Recognition by Pathogen Lectins. ACS Chem Biol. 2016 Jul 15;11(7):2011-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8886 mL | 9.4430 mL | 18.8861 mL | 47.2152 mL |
| 5 mM | 0.3777 mL | 1.8886 mL | 3.7772 mL | 9.4430 mL | |
| 10 mM | 0.1889 mL | 0.9443 mL | 1.8886 mL | 4.7215 mL | |
| 15 mM | 0.1259 mL | 0.6295 mL | 1.2591 mL | 3.1477 mL | |
| 20 mM | 0.0944 mL | 0.4722 mL | 0.9443 mL | 2.3608 mL | |
| 25 mM | 0.0755 mL | 0.3777 mL | 0.7554 mL | 1.8886 mL | |
| 30 mM | 0.0630 mL | 0.3148 mL | 0.6295 mL | 1.5738 mL | |
| 40 mM | 0.0472 mL | 0.2361 mL | 0.4722 mL | 1.1804 mL | |
| 50 mM | 0.0378 mL | 0.1889 mL | 0.3777 mL | 0.9443 mL | |
| 60 mM | 0.0315 mL | 0.1574 mL | 0.3148 mL | 0.7869 mL | |
| 80 mM | 0.0236 mL | 0.1180 mL | 0.2361 mL | 0.5902 mL | |
| 100 mM | 0.0189 mL | 0.0944 mL | 0.1889 mL | 0.4722 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Lewis X trisaccharide Related Classifications
HY-N10534 Related Classifications
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