PNCK Protein, Human (Active, sf9, His, GST)

The PNCK protein is a calcium/calmodulin-dependent protein kinase and a key component of the calcium-triggered signaling cascade. In vitro, PNCK exhibits the ability to phosphorylate CREB1 and SYN1/synapsin I, emphasizing its involvement in regulating cellular processes associated with these substrates. PNCK Protein, Human (sf9, His, GST) is the recombinant human-derived PNCK protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

The PNCK protein is identified as a calcium/calmodulin-dependent protein kinase, suggesting its involvement in a proposed calcium-triggered signaling cascade. In vitro studies have revealed that PNCK has the ability to phosphorylate CREB1 and SYN1 (synapsin I), indicating a role in modulating cellular processes associated with synaptic function and gene expression. Moreover, PNCK phosphorylates and activates CAMK1, suggesting its participation in the regulation of other calcium/calmodulin-dependent kinases. These findings highlight PNCK's potential significance in cellular signaling pathways, particularly those related to calcium-mediated events, and underscore its role in the phosphorylation and activation of key proteins involved in synaptic function and gene regulation. Further research is needed to elucidate the specific biological contexts and physiological consequences of PNCK-mediated phosphorylation events.

Human

Sf9 insect cells

N-8*His;N-GST

Q6P2M8-1 (L2-W343)

139728

Partial

LLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEALVENEIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYLHSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWALGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALRHLWISGDTAFDRDILGSVSEQIRKNFARTHWKRAFNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRAGQPPKW

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.