PNCK Protein, Human (Active, sf9, His, GST)
The PNCK protein is a calcium/calmodulin-dependent protein kinase and a key component of the calcium-triggered signaling cascade. In vitro, PNCK exhibits the ability to phosphorylate CREB1 and SYN1/synapsin I, emphasizing its involvement in regulating cellular processes associated with these substrates. PNCK Protein, Human (sf9, His, GST) is the recombinant human-derived PNCK protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PNCK protein is a calcium/calmodulin-dependent protein kinase and a key component of the calcium-triggered signaling cascade. In vitro, PNCK exhibits the ability to phosphorylate CREB1 and SYN1/synapsin I, emphasizing its involvement in regulating cellular processes associated with these substrates. PNCK Protein, Human (sf9, His, GST) is the recombinant human-derived PNCK protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Background
The PNCK protein is identified as a calcium/calmodulin-dependent protein kinase, suggesting its involvement in a proposed calcium-triggered signaling cascade. In vitro studies have revealed that PNCK has the ability to phosphorylate CREB1 and SYN1 (synapsin I), indicating a role in modulating cellular processes associated with synaptic function and gene expression. Moreover, PNCK phosphorylates and activates CAMK1, suggesting its participation in the regulation of other calcium/calmodulin-dependent kinases. These findings highlight PNCK's potential significance in cellular signaling pathways, particularly those related to calcium-mediated events, and underscore its role in the phosphorylation and activation of key proteins involved in synaptic function and gene regulation. Further research is needed to elucidate the specific biological contexts and physiological consequences of PNCK-mediated phosphorylation events.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His;N-GST
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Accession
Q6P2M8-1 (L2-W343)
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Gene ID139728
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Molecular Construction
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N-term
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8*His-GST
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PNCK (L2-W343)
Accession # Q6P2M8-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
PNCK; CaM Kinase IB; Pregnancy Up-Regulated Nonubiquitous CaM Kinase; CaM-KI Beta; Calcium/Calmodulin-Dependent Protein Kinase Type 1B; CaMKI-Beta; CaM Kinase I Beta; MGC45419; CaMK1b; Pregnancy Up-Regulated Non-Ubiquitously Expressed CaM Kinase; Pregnancy Up-Regulated Non-Ubiquitously-Expressed CaM Kinase; BSTK3; Pregnancy Upregulated Non-Ubiquitously Expressed CaM Kinase
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AA Sequence
LLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEALVENEIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYLHSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWALGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALRHLWISGDTAFDRDILGSVSEQIRKNFARTHWKRAFNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRAGQPPKW
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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