7-Benzyloxy-4-(trifluoromethyl)coumarin
Based on 1 Customer Validation
7-Benzyloxy-4-(trifluoromethyl) coumarin (7-BFC) is a coumarin-based fluorescent substrate. 7-Benzyloxy-4-(trifluoromethyl) coumarin serves as a substrate for cDNA-expressed CYP1A2 and CYP3A4, and is metabolized into 7-hydroxy-4-trifluoromethylcoumarin (HFC). 7-Benzyloxy-4-(trifluoromethyl) coumarin supports metabolic studies of cytochrome P450 subtypes via its fluorescent metabolite.
For research use only. We do not sell to patients.
- Purity : 99.47%
- CAS No.: 220001-53-6
- Formula: C17H11F3O3
- Molecular Weight:320.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CYP1A2 |
CYP3A4 |
In Vitro
7-Benzyloxy-4-(trifluoromethyl)coumarin (2-100 μM; 15 min) is metabolized to HFC in pooled liver microsomes with a mean Km of 8.3 μM and mean Vmax of 454 pmol/min/mg protein[1].
7-Benzyloxy-4-(trifluoromethyl)coumarin (20 μM; 15 min) metabolism in pooled liver microsomes is inhibited by CYP1A2 and CYP3A4 inhibitors and anti-CYP3A4 antibody, confirming primary catalysis by CYP1A2 and CYP3A4[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 220001-53-6
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Appearance Solid
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Molecular Weight 320.27
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Formula C17H11F3O3
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Color White to off-white
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SMILES
C1=CC=C(C=C1)COC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F
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Synonyms
7-BFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (390.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1224 mL | 15.6118 mL | 31.2237 mL | 78.0591 mL |
| 5 mM | 0.6245 mL | 3.1224 mL | 6.2447 mL | 15.6118 mL | |
| 10 mM | 0.3122 mL | 1.5612 mL | 3.1224 mL | 7.8059 mL | |
| 15 mM | 0.2082 mL | 1.0408 mL | 2.0816 mL | 5.2039 mL | |
| 20 mM | 0.1561 mL | 0.7806 mL | 1.5612 mL | 3.9030 mL | |
| 25 mM | 0.1249 mL | 0.6245 mL | 1.2489 mL | 3.1224 mL | |
| 30 mM | 0.1041 mL | 0.5204 mL | 1.0408 mL | 2.6020 mL | |
| 40 mM | 0.0781 mL | 0.3903 mL | 0.7806 mL | 1.9515 mL | |
| 50 mM | 0.0624 mL | 0.3122 mL | 0.6245 mL | 1.5612 mL | |
| 60 mM | 0.0520 mL | 0.2602 mL | 0.5204 mL | 1.3010 mL | |
| 80 mM | 0.0390 mL | 0.1951 mL | 0.3903 mL | 0.9757 mL | |
| 100 mM | 0.0312 mL | 0.1561 mL | 0.3122 mL | 0.7806 mL |
7-Benzyloxy-4-(trifluoromethyl)coumarin Related Classifications
HY-W267897 Related Classifications
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