Lotion Exosome extraction kit

The discovery of hit molecule is a cornerstone of drug development. Among the diverse tools available, DNA-encoded libraries have emerged a revolutionary platform for high-throughput screening. Compared with traditional HTS, DEL features shorter screening processes, lower costs, simpler assays, and larger library capacities.

DEL Construction utilizes split-and-pool synthesis, a combinatorial chemistry approach that involves iterative splitting, reaction, and pooling. This strategy enables rapid, exponential assembly of fragments in minimal steps without the need for individual compound synthesis andassoicicated isolation or purification steps, thus greatly reducing overall costs. The technology enables simultaneous affinity screeningof massive compound collections to target proteins in a single step. By coupling chemical structures with unique DNA barcodes, each compound is tagged with a distinct DNA sequence for convenient tracking and decoding.DELs readily enable the construction and efficient screening of libraries containing millions to billions of compounds. As a result, DEL screening combines the dual advantages of high efficiency and low cost, making DEL a transformative technology in modern drug discovery.

The DEL kit consists of 50 independent libraries with a total scale of 100 billion compounds. It is constructed through stepwise combinatorial chemistry strategies involving 2-, 3-, and 4-round synthesis. By employing diverse scaffolds and flexible linking strategies, it encompasses various ring systems, linear frameworks, and heterocyclic structures. Screening can be achieved solely through affinity, independent of target-specific activity detection methods. This library is suitable for DEL screening against a wide range of targets.

Exosomes are small membrane vesicles of endocytic origin that are secreted by most cells in culture. Exosomes contain nucleic acids, proteins, lipids, amino acids, and metabolites, etc. Their diverse constituents can reflect their cell of origin. Exosomes are associated with immune responses, viral pathogenicity, pregnancy, cardiovascular diseases, central nervous system-related diseases, and cancer progression. Proteins, metabolites, and nucleic acids delivered by exosomes into recipient cells effectively alter their biological response. Such exosome-mediated responses can be disease promoting or restraining.

The biology of exosomes in disease is still emerging, and the number of studies addressing their utility in the diagnosis and treatment of various pathologies has increased substantially. MCE supplies a unique collection of 53 compounds with the activity of inhibiting or stimulating exsomes secretion/biosynthesis. MCE Exosomes Compound Library is a useful tool for exsomes research.

Cyclic peptide library have advantages such as high affinity, high selectivity, and suitability for targeting protein–protein interactions. Through DEL synthesis technology, the library size can achieve hundreds of millions. DEL cyclic peptide library have advantages like low cost andhigh screeing efficiency, making them valuable for discovering lead compounds against challenging drug targets.

This cyclic peptide library is constructed with unnatural amino acids as building block, synthesized through DNA-compatible chemical reactions. Each cyclic peptide consist of six amino acids and constrained conformations such as side-chain cross-linking, disulfide bonds, and macrocyclization. These cyclic peptides exhibit significantly improved stability and druggability compared with linear peptides, filling the gap between small molecules and macromolecular biologics. Each cyclic peptide is uniquely conjugated to a DNA barcode sequence for molecular identification and sequencing decoding.

MCE’s cyclic peptide library has8 independent sub-libraries, with a total molecular diversity of 1.2 billion. It is constructed via multi-round combinatorial assembly of building blocks and diverse cyclization strategies, facilitating the discovery of cyclic peptide leads for undruggable targets.

Extracellular vesicles (EVs) are small membrane binding structures that are released from cells into the surrounding environment and play a crucial role in mediating and regulating intercellular communication related to physiological and pathological processes. EVs are lipid membrane vesicles composed of proteins, lipids, and nucleic acids. EVs can be divided into several types based on their source, such as extracellular vesicles, microcapsules, and apoptotic vesicles. The size range of exosomes is 30-150nm, which are endocrine in multi vesicular endosomes (MVEs); microvesicles (50-1000nm) are secreted directly through extracellular interactions, thereby releasing plasma membrane vesicles. In contrast, apoptotic bodies are usually larger, ranging in size from 1 to 5 μ m. This is generated during programmed cell death. EV plays a crucial role in transmitting information between cells and influencing the behavior and function of receptor cells.

MCE designs a unique collection of 643 small molecules related to extracellular vesicles (EVs). It is a good tool to be used for research on metabolize, cancer and other diseases.

MCE Exosome Protein Detection Kit (CD81) can specifically detect the exosome marker protein CD81.

MCE Exosome Isolation and Purification Kit (From Milk) provides a simple and effective method to isolate and purify intact exosomes from milk.

MCE Exosome Isolation and Purification Plus Kit (From Plasma and Serum) provides a simple and effective method to isolate and purify intact exosomes from plasma and serum

MCE Exosome Protein Detection Kit (CD63&TSG101) can specifically detect exosome proteins CD63 and TSG101. This product is suitable for the detection of human, rat and mouse exosomes.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from plasma and serum that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cell culture media that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Exosome Isolation and Purification Kit (From Tissue) provides a simple and effective method to isolate and purify intact exosomes from a variety of tissues (such as brain, heart, lung, liver, muscle, lymph nodes, thymus, embryo, tumor, etc.).

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cerebro-spinal fluid, amniotic fluid, milk, saliva, etc, which can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Plant Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from various plant leaf samples. The kit enables rapid release of genomic DNA from plant tissues (such as rice, maize, tobacco, rapeseed, etc.) in a single step. The resulting lysate can be directly used for downstream PCR amplification without the need for conventional DNA extraction and purification, thereby significantly simplifying the experimental workflow.

MCE Blood Direct PCR Kit (with Dye) is specifically designed for direct amplification from blood samples. It enables PCR amplification directly from whole blood without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the workflow and improving detection efficiency.

MCE Exosome Protein Lysis Buffer is a lysis buffer specifically designed for exosome samples, capable of efficiently lysing exosome proteins.

MCE Mouse Tissue Direct PCR Kit – Pro (with Dye) is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

MCE Mouse Tissue Direct PCR Kit (with Dye) is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

MCE Mouse Tissue Direct PCR Plus Kit is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

MCE Exosome-Specific Culture Medium (Serum-Free, Phenol Red-Free) is specifically formulated for optimized exosome collection.

MCE Exosome-Specific 293 Cell Medium (Serum-Free) is specifically optimized for exosome production from HEK293 cells.

MCE Exosome Preservation Solution (Chemically Defined) is a buffer specifically formulated for the storage of exosome samples, designed to maintain their morphological integrity and biological activity.

MCE Animal Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from a variety of animal tissue samples. It enables rapid release of genomic DNA from animal tissues (such as insect legs/wings, mouse tails, ears, toes, skin, and internal organs) in a single step, allowing the lysate to be used directly for downstream PCR amplification without the need for conventional DNA extraction and purification procedures, thereby significantly simplifying the workflow.

MCE Exosome-Specific Mesenchymal Stem Cell Medium (Serum-Free, Phenol Red-Free) is specifically optimized for the production and preparation of exosomes from mesenchymal stem cells (MSCs).

MCE Exosome-Specific 293 Cell Medium Supplement is designed as a feed supplement for use with the Exosome-Specific 293 Cell Medium (Serum-Free) (MCE Cat. No.: HY-K3113).

MCE Mammalian Active Protein Extraction Reagent are gentle, non-denaturing detergents designed for the rapid, high-quality, and high-activity extraction of cytoplasmic, nuclear, and membrane proteins from mammalian cells or tissues. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Organoid Vitality Assay Kit is a fluorescence-based assay kit specifically designed for measuring cell viability in organoids.

MCE Mycoplasma Detection Kit (PCR) is a diagnostic kit that utilizes the PCR method to detect mycoplasma contamination in biological materials such as cultured cells.

MCE Milk-derived exosomes are high-purity extracellular vesicles isolated and purified from fresh milk, with diameters ranging from approximately 30 to 200 nm.

MCE ATP Assay Kit provides a rapid method to measure intracellular ATP. The kit is suitable for ATP determination in cells, tissue, and other biological samples.

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

The MCE three-color immunohistochemical kit marks the dual indicators. The kit contains rabbit secondary antibodies and can be used in combination with rabbit primary antibodies

The MCE four-color immunohistochemical kit can label three indicators. The kit contains rabbit secondary antibodies and can be used in combination with rabbit primary antibodies.

MCE Mycoplasma Detection Kit (qPCR) is a diagnostic kit that utilizes the real-time quantitative PCR (qPCR) method to detect mycoplasma contamination in biological materials such as cultured cells.

MCE Hydroxyl Magnetic beads (200 nm, 10 mg/mL) can rapidly isolate nucleic acids from biological samples, which is conducive to the automation and high throughput extraction of nucleic acids.

The MCE three-color immunohistochemical kit marks the dual indicators. The kit contains a universal secondary antibody for mice and rats and can be used in combination with rabbit or mouse primary antibodies.

The MCE four-color histochemical kit can label three indicators. The kit contains a universal secondary antibody for rabbits and mice and can be used in combination with primary antibodies for rabbits or mice.

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

MCE Human iPSC/ESC Whole-Brain Organoid Induction Differentiation Kit is a non-region-specific brain organoid induction kit designed based on the neuroectoderm self-differentiation system. After 38 d of culture using this kit, the resulting whole-brain organoids stably express core neuronal markers such as TUJ1, SOX2, Nestin, and NeuN, as well as forebrain/cortical markers including FOXG1 and CTIP2.

MCE Mouse Intestinal Organoid Plus Kit is an upgraded serum-free culture system developed based on the Mouse Small Intestinal Organoid Culture Kit (HY-K6119), designed for the establishment, expansion, and long-term maintenance of mouse small intestinal organoids. The kit contains basal culture medium and multiple essential supplements, and an optional Wnt ligand supplement is provided to enable modulation of Wnt signaling activity in the culture system.

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.The 5 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE ROS Assay Kit utilizes the fluorescent probe DCFH-DA to detect ROS.

MCE Lentivirus Packaging Kit is based on the second-generation lentiviral packaging system and is also compatible with both second- and third-generation packaging plasmids. Lentiviruses produced using this kit exhibit high infection efficiency and a broad host range, enabling stable and sustained expression of exogenous genes in host cells.

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH₂O) protects protein from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis.

MCE Gram Stain Kit can be used to differentiate between Gram-negative and Gram-positive bacteria.

MCE Cell Senescence β-Galactosidase Staining Kit can be used to evaluate the senescence of cells or tissues.

MCE Cell Apoptosis Analysis Kit (Hoechst staining) provides a rapid and convenient method to detect cell apoptosis.

MCE Antibody Labeling Kit-VF 488 can efficiently and covalently attach the VF 488 dye to antibodies.

MCE Human iPSC/ESC Cardiomyocyte Induction Differentiation Kit is based on the classical GiWi system. By precisely modulating the Wnt/β-catenin signaling pathway in a temporally controlled manner (sequential activation and inhibition), the kit enables highly efficient directed differentiation of human pluripotent stem cells into cardiomyocytes. It is suitable for cardiac disease modeling, drug cardiotoxicity assessment, and mechanistic studies.

MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.

MCE Nitric Oxide Detection Kit employs a modified Griess method to detect the content of Nitric Oxide in various samples.