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Cat. No.: HY-P2963

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Nuclease P1

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

For research use only. We do not sell to patients.

CAS No.: 54576-84-0

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Please store the product under the recommended conditions in the Certificate of Analysis.

Nuclease P1 Chemical Structure

CAS No.: 54576-84-0

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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- In Vitro
- EC Number
- Specific Activity
- Unit Definition
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
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Biological Activity

Description

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries^[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

All Endogenous Metabolite Isoforms :

In Vitro

Reaction condition
pH range: Effective pH range: 4.0-8.0, optimal pH range: 4.5-5.5
Temperature range: Effective temperature range: 35°C-85°C, optimal temperature range: 65°C-75°C

Protocol Yeast nucleic acid (RNA) is used as the substrate, the substrate concentration is 2%-5%, the recommended enzyme addition amount is 2%-4%(based on the dry matter of the substrate), and the enzymatic hydrolysis time under the optimal conditions is 7 h-9 h;
Yeast is used as the substrate, the yeast concentration is 10%-12%, the nuclease amount is 0.3%-0.5%(based on dry yeast matter), and the enzymatic hydrolysis time under the optimal conditions is 7 h-9 h.
Note: Due to differences in application fields and experimental parameters, the actual addition method and amount of this product should be determined through testing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.1.30.1

Specific Activity

≥200 U/mg protein

Unit Definition

3' 5”-Phosphodiesterase: One unit is defined as the amount of enzyme that releas 1 μmol acidsoluble nucleotides from RNA at 37°C and pH 5.3, in per minute；3`-Nuclease: One unit is defined as the amount of enzyme that hydrolyze 1 μmol of orthophosphoric acid from 3`-AMP in per minute, at 37°C and pH 7.2

Chemical Information

CAS No. 54576-84-0
Appearance Solid
Color White to light yellow
SMILES

[Nuclease P1]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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SSRN

2026 Mar 2.

Solvent & Solubility

In Vitro:

H₂O : 4.17 mg/mL (ultrasonic and warming and heat to 37°C)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- In Vitro
- EC Number
- Specific Activity
- Unit Definition
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References

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Nuclease P1

Publications Citing Use of MedChemExpress (MCE) Nuclease P1

All Endogenous Metabolite Isoforms