Anti-c-Kit/CD117 Antibody (SR-1)

Cat. No.: HY-P990851 Purity: ≥99.0%: Data Sheet
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Anti-c-Kit/CD117 Antibody (SR-1) is a kind of mouse IgG2a κ chimeric antibody inhibitor, targeting to human c-Kit/CD117. Anti-c-Kit/CD117 Antibody (SR-1) binds to the extracellular domain of human c-Kit (also known as CD117) and blocks stem cell factor (SCF) binding to CD117. Anti-c-Kit/CD117 Antibody (SR-1) can be used for the researches of cancer and immunology, such as gastrointestinal stromal tumor (GIST) and xenograft.

For research use only. We do not sell to patients.

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Mouse IgG2a kappa

Recommend Isotype Controls

Mouse IgG2a kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

c-Kit/CD117

In Vitro

Anti-c-Kit/CD117 Antibody (SR-1) (10 μg/mL, 12 h) inhibits cell growth and reduces cell-surface KIT expression in GIST48/GIST430/GIST882 cells^[1].
Anti-c-Kit/CD117 Antibody (SR-1) (10 μg/mL, 2 h) allows for phagocytosis of gastrointestinal stromal tumor (GIST) cells by macrophages in GIST48/GIST430/GIST882 and LMS05 cells co-cultures^[1].
Anti-c-Kit/CD117 Antibody (SR-1) (0.01-100 μg/mL, 2 weeks) inhibits cells proliferation in human hematopoietic stem cells (HSC)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-c-Kit/CD117 Antibody (SR-1) (500 μg, i.p., weekly 2 weeks after cells engraftment) inhibits tumor growth in GIST430/GIST882 xenograft tumor NSG mice^[1].
Anti-c-Kit/CD117 Antibody (SR-1) (500 μg, i.v., at days 1, 3, 5, and 7) depletes normal and myelodysplastic syndrome human hematopoietic stem cells (HSC) in HSC xenografted NSG mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	GIST430 xenograft tumor mice models of imatinib (HY-15463)-resistant and GIST882 xenograft tumor mice models of imatinib-sensitive (4-8 weeks, NSG mice)^[1]
Dosage:	500 μg
Administration:	Intraperitoneally injection, weekly 2 weeks after cells engraftment
Result:	Inhibited tumor growth.

Animal Model:	Human hematopoietic stem cells (HSC)-xenografted NSG mice (prenatal day 0 (P0) to P3 newborn pups) ^[2]
Dosage:	500 μg
Administration:	Intravenously injection, at days 1, 3, 5, and 7
Result:	Depleted human CD451CD13/331 cells. Depleted cells from high and low-risk MDS subtypes. Generated human myeloid and lymphoid cells in the BM. Allowed subsequent engraftment of healthy human HSCs and restored of normal human hematopoiesis.

Gene ID

16590 [NCBI]

Accession

P05532

Application

in vivo c-Kit+ cell depletionin vitro c-Kit targetingImmunohistochemistry (frozen)

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

146.12 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-c-Kit/CD117 Antibody (SR-1)]

Shipping

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Biological Activity

Immobilized KIT-D1-D3-His can bind Anti-c-Kit/CD117 Antibody (SR-1). The EC₅₀ for this effect is 42.8 ng/mL.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (261 KB) SDS (251 KB)

COA (232 KB) SDS-PAGE (63 KB) SEC-HPLC (77 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Edris B, et al. Anti-KIT monoclonal antibody inhibits imatinib-resistant gastrointestinal stromal tumor growth. Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3501-6. [Content Brief]

[2]. Pang WW, et al. Anti-CD117 antibody depletes normal and myelodysplastic syndrome human hematopoietic stem cells in xenografted mice. Blood. 2019 May 9;133(19):2069-2078. [Content Brief]

Anti-c-Kit/CD117 Antibody (SR-1) Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-c-Kit/CD117 Antibody (SR-1)c-KitSCFRCD117C-KitSCFGISTXenograftHSCGIST48GIST430GIST882Inhibitorinhibitorinhibit

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