Ramiprilat acyl-β-D-glucuronide 

Ramiprilat acyl-β-D-glucuronide is an acyl glucuronide metabolite of Ramiprilat (HY-B0279). Ramiprilat acyl-β-D-glucuronide undergoes methylation on its glucuronic acid moiety during solid phase extraction with strong cation exchanger cartridges, forming a methylated derivative. Ramiprilat acyl-β-D-glucuronide undergoes in-source conversion to Ramiprilat in mass spectrometry, with conversion extent proportional to declustering potential voltage^[1].

Ramiprilat acyl-β-D-glucuronide (10.00-100.00 μg/mL) is chromatographically separable from ramiprilat under initial reversed-phase HPLC conditions, does not cause ramiprilat quantification variability via in-source conversion, and undergoes a 65% loss with concurrent methylated derivative formation when processed using strong cation exchanger solid-phase extraction^[1].
Ramiprilat acyl-β-D-glucuronide (10.00-100.00 μg/mL) undergoes hydrolysis during evaporation in protein precipitation sample preparation, with reduced hydrolysis observed at an evaporation temperature of 35°C compared to 50°C^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

564.58

C₂₇H₃₆N₂O₁₁

1357570-22-9

OC([C@H](CCC1=CC=CC=C1)N[C@@H](C)C(N2[C@]3([H])[C@](CCC3)([H])C[C@H]2C(O[C@@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)C(O)=O)=O)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Côté C, et al. Impact of methylation of acyl glucuronide metabolites on incurred sample reanalysis evaluation: ramiprilat case study. Bioanalysis. 2011;3(9):951-965.  [Content Brief]