1. Metabolic Enzyme/Protease
    Apoptosis
  2. Angiotensin-converting Enzyme (ACE)
    Apoptosis
  3. Ramipril

Ramipril (Synonyms: HOE-498)

Cat. No.: HY-B0279 Purity: 99.81%
Handling Instructions

Ramipril (HOE-498) is an angiotensin-converting enzyme (ACE) inhibitor with IC50 of 5 nM.

For research use only. We do not sell to patients.

Ramipril Chemical Structure

Ramipril Chemical Structure

CAS No. : 87333-19-5

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 90 In-stock
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Description

Ramipril (HOE-498) is an angiotensin-converting enzyme (ACE) inhibitor with IC50 of 5 nM.

IC50 & Target

ACE[1].

In Vitro

Ramipril (HOE-498) is an angiotensin-converting enzyme (ACE) inhibitor with IC50 of 5 nM[1]. Ramipril (HOE-498) enhances the activity of ACE-associated CK2 and the phosphorylation of ACE Ser1270 in cultured endothelial cells, but is unable to activate JNK or stimulate the nuclear accumulation of c-Jun in endothelial cells expressing a S1270A ACE mutant or in ACE-deficient cells. Prolonged Ramipril treatment increases ACE expression in primary cultures of human endothelial cells and in vivo (mouse lung), which can be prevented by pretreatment with the JNK inhibitor SP600125[2].

In Vivo

Chronic in vivo administration of Ramipril (HOE-498) to rats at a dosage that has similar hypotensive effects in vitro HUVECs significantly reduces the rate of LPS-induced apoptosis compared to the other ACE inhibitors, which contrasts with the apoptosis effect in vitro[3]. Ramipril (HOE-498) inhibits systolic blood pressure (SBP) with IC50 of 1.97 mg/kg in spontaneously hypertensive rats (SHR). When in combination with AT1-receptor blockade by candesartan-cilexetil increases SBP reduction synergistically rather than additively[4].

Clinical Trial
Molecular Weight

416.51

Formula

C₂₃H₃₂N₂O₅

CAS No.

87333-19-5

SMILES

O=C([[email protected]@H]1C[[email protected]@](CCC2)([H])[[email protected]@]2([H])N1C([[email protected]@H](N[[email protected]](C(OCC)=O)CCC3=CC=CC=C3)C)=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (240.09 mM)

H2O : 1 mg/mL (2.40 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4009 mL 12.0045 mL 24.0090 mL
5 mM 0.4802 mL 2.4009 mL 4.8018 mL
10 mM 0.2401 mL 1.2005 mL 2.4009 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3.25 mg/mL (7.80 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 3.25 mg/mL (7.80 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 3.25 mg/mL (7.80 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

RamiprilHOE-498HOE498HOE 498Angiotensin-converting Enzyme (ACE)ApoptosisInhibitorinhibitorinhibit

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Ramipril
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