2. Anti-infection
  3. Bacterial Fungal Influenza Virus Antibiotic
  4. Tunicamycin

Tunicamycin 

Cat. No.: HY-A0098 Purity: 99.96%
COA Handling Instructions

Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity.Tunicamycin increases exosome release in cervical cancer cells.

For research use only. We do not sell to patients.

Tunicamycin Chemical Structure

Tunicamycin Chemical Structure

CAS No. : 11089-65-9

Size Price Stock Quantity
2 mg USD 190 In-stock
5 mg USD 385 In-stock
10 mg USD 650 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 69 publication(s) in Google Scholar

Tunicamycin Related Antibodies

Top Publications Citing Use of Products

67 Publications Citing Use of MCE Tunicamycin

WB
Proliferation Assay

    Tunicamycin purchased from MCE. Usage Cited in: PLoS Biol. 2018 Oct 18;16(10):e2006483.   [Abstract]

    SK-Hep1 cells stably expressing EV or Flag-PGM1 are treated with or without 0.1 ug/mL Tunicamycin, and cell proliferation is measured.

    Tunicamycin purchased from MCE. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):604.  [Abstract]

    Western blot analysis of ER stress-related proteins after PPI treatment for 48 h in both pH 7.4 and pH 6.5 conditions. Cells treated with Thapsigargin (TG, 0.5 and 1 μM) or Tunicamycin (Tu, 2.5, 5 and 10 μg/mL) for 24 h served as positive controls.

    View All Antibiotic Isoform Specific Products:

    View All Isoforms
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity[1][2][3].Tunicamycin increases exosome release in cervical cancer cells[4].

    In Vitro

    Tunicamycin (2 μg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells[1].
    Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Tunicamycin Related Antibodies

    Western Blot Analysis

    Cell Line: CD44+/CD24- and original MCF7 cells[1]
    Concentration: 2 µg/mL
    Incubation Time: 24 hours
    Result: Increased level of spliced XBP-1, ATF6 nuclear translocation and CHOP protein expression are detected in CD44+/CD24- and original MCF7 cells.
    In Vivo

    Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133+/- MHCC97L cells xenograft model (BALB/c (nu/nu) mice)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    844.94 (n=10)

    Appearance

    Solid

    Formula

    C39H64N4O16
    CAS No.
    SMILES

    O=C1NC(N([C@H]2[C@H](O)[C@H](O)[C@@H](C(C[C@@H]3[C@H](O)[C@H](O)[C@@H](NC(/C=C/[(CH2) 8-11]C(C)C)=O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O3)O)O2)C=C1)=O
    Structure Classification
    Initial Source

    Streptomyces lysosuperficus
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.96%

    COA (260 KB) LCMS (269 KB)

    Handling Instructions (2659 KB)
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Tunicamycin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Tunicamycin11089-65-9BacterialFungalInfluenza VirusAntibioticERUDP-HexNAcantibioticglycoproteinN-linkedDNAsynthesisanti-cancerXBP-1ATF6invasionInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Tunicamycin
    Cat. No.:
    HY-A0098
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.