18:0,18:1 PS
Based on 1 Customer Validation
18:0,18:1 PS is the dominant phosphatidylserine subtype in cells, exosomes and HIV particles. It is abundant in the brain and is essential for maintaining membrane structure, lipid raft organization and intracellular trafficking. 18:0,18:1 PS mediates interleaflet membrane coupling through cholesterol-dependent interactions with very long-chain sphingolipids, and can induce the clustering of glycosylphosphatidylinositol-anchored proteins. In addition, clusters formed by the binding of 18:0,18:1 PS to cholesterol not only facilitate the proper distribution of cholesterol in lipid bilayers, but also effectively protect cholesterol from oxidative damage.
For research use only. We do not sell to patients.
- Purity : 99.95%
- CAS No.: 124262-93-7
- Formula: C42H80NO10P
- Molecular Weight:790.06
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All Endogenous Metabolite Isoforms
More
Biological Activity
Description
In Vitro
18:0,18:1 PS is the dominant phosphatidylserine subtype in cells, exosomes and HIV particles[1]:
(1) PS 18:0,18:1 accounts for approximately 22-60% of total PS in various cell lines including PC-3, HEp-2, PSA3 CHO, HeLa, MT4, A549 and mouse fibroblast cell lines.
(2) PS 18:0,18:1 accounts for approximately 40% of total PS species in exosomes derived from PC-3 cells, and theoretically covers about 80% of the inner leaflet area overlapped by very long-chain sphingolipids on the outer leaflet[1].
(3) PS 18:0,18:1 theoretically covers about 60% of the inner leaflet area overlapped by very long-chain sphingolipids on the outer leaflet of HIV-1 particles.
The cross-intercalation between PS 18:0,18:1 and SM d18:1/24:0 is stronger than that between PS 16:0/18:1 (HY-146885), and it is the only tested PS species whose cross-intercalation with SM d18:1/24:0 is enhanced in the presence of cholesterol[1].
PS 18:0,18:1 can co-drive phase separation with cholesterol in GUVs, and more effectively protect cholesterol from the action of cholesterol oxidase compared with PS 16:0/18:1, PS 18:1/18:1 and PS 16:0/18:2[1].
PS 18:0,18:1 promotes the clustering of endogenous GPI-APs on the surface of PSA3 CHO cells, with an efficacy comparable to that of PS 18:0/18:0 and more potent than that of PS 18:1/18:1 or PS 12:0/12:0[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 124262-93-7
-
Appearance Solid
-
Molecular Weight 790.06
-
Formula C42H80NO10P
-
Color White to off-white
-
SMILES
OC([C@@H](N)COP(OC[C@@H](COC(CCCCCCCCCCCCCCCCC)=O)OC(CCCCCCC/C=C\CCCCCCCC)=O)(O)=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Protocols
-
ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
-
Data Sheet (265 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Skotland T, et al. The role of PS 18:0/18:1 in membrane function. Nat Commun. 2019;10(1):2752. Published 2019 Jun 21. [Content Brief]
[2]. Choi J, et al. Comprehensive analysis of phospholipids in the brain, heart, kidney, and liver: brain phospholipids are least enriched with polyunsaturated fatty acids. Mol Cell Biochem. 2018;442(1-2):187-201. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
18:0,18:1 PS Related Classifications
HY-148978A Related Classifications
Powered by Bioz