Ribonucleoside vanadyl complexes
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Ribonucleoside vanadyl complexes are a class of potent RNase and Taq polymerase inhibitors. Ribonucleoside vanadyl complexes protect RNA during RNA isolation by inhibiting ribonucleases, and also reduce the viability of bacteria and eukaryotic cells by interfering with ribosomal subunit assembly. Ribonucleoside vanadyl complexes block PCR and reverse transcription reactions templated by viral nucleic acids and enhance the effects of antibiotics against Staphylococcus aureus, but do not directly inhibit protein synthesis. Ribonucleoside vanadyl complexes can be effectively removed by phenol-chloroform extraction, thus enabling subsequent PCR analysis. Ribonucleoside vanadyl complexes can be applied in research related to chronic hepatitis C (HCV) and Staphylococcus aureus infection.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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- References
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Help & FAQs
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Biological Activity
Ribonucleoside vanadyl complexes (RVC) (0.04-8 mM) potently inhibit PCR reactions using both HCV cDNA and HBV DNA templates, with consistent inhibition observed at concentrations of 0.4 mM and higher, while 0.04 mM has no inhibitory effect[1].
Ribonucleoside vanadyl complexes (RVC) (20 mM; 4-5 days) inhibits RT-nPCR detection of HCV RNA, even when positive HCV RNA is spiked into the reaction, when included during prolonged proteinase K digestion of formalin-fixed paraffin-embedded liver tissue[1].
Ribonucleoside vanadyl complexes (5 mM) reduced the viable cell counts of methicillin-resistant (HY-121544) MSSA RN1786 and MRSA A1024 by >90%[2].
Ribonucleoside vanadyl complexes (5 mM) reduces ribosomal subunit formation by 90%, reduces 50S subunit relative abundance by ~15% in MSSA cells, and increases rRNA degradation in both MSSA RN1786 and MRSA A1024 cells[2].
Ribonucleoside vanadyl complexes (5 mM) reduces the synthesis rate of both 30S and 50S ribosomal subunits, including a 4-fold reduction in 50S subunit synthesis, in MSSA RN1786 and MRSA A1024 cells[2].
Ribonucleoside vanadyl complexes (1-5 mM; ~34 h) reduce viable cell counts by ~90%-98% in RAW 264.7 macrophage and BJ fibroblast cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Methicillin-susceptible Staphylococcus aureus (MSSA) RN1786, methicillin-resistant Staphylococcus aureus (MRSA) A1024
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Concentration:5 mM
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Incubation Time:matched growth period for viability assessment (added after 1 h initial growth)
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Result:Decreased cell viability by >90% in both MSSA and MRSA strains.
Reduced MSSA cfu to 11×107/mL (5.3% of control) and MRSA cfu to 5×107/mL (1.6% of control), with statistically significant differences from untreated cells.
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Cell Line:RAW 264.7 macrophage, BJ fibroblast
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Concentration:0.5 mM, 1 mM, 5 mM
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Incubation Time:~34 h total post-addition (added after 2 h initial growth for macrophages or 12 h initial growth for fibroblasts)
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Result:Reduced viable cell number by ~90%-98% in both macrophage and fibroblast cultures at 1 mM and 5 mM concentrations.
Chemical Information
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Appearance Liquid
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Color Dark blue to black
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SMILES
[Ribonucleoside vanadyl complexes]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ribonucleoside vanadyl complexes Related Classifications
HY-E70529 Related Classifications
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