Mouse CD3⁺ T Cells Negative Selection Kit

MCE Mouse CD3⁺ T Cells Negative Selection Kit is designed for the isolation of CD3⁺ T cells from single cell suspensions of mouse spleen cells and lymph nodes. Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3⁺ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3⁺ T cells.

The kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

Features of MCE Mouse CD3⁺ T Cells Negative Selection Kit

•   Efficiency: Enables sorting of target cells in as little as 15 minutes;

•   Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

•   High Purity: Can yield sorted cells with a purity exceeding 95%;

•   High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.