2. MCE Kits
  3. Cell Biology
  4. Cell Isolation
  5. Mouse CD3+ T Cells Negative Selection Kit

Mouse CD3+ T Cells Negative Selection Kit 

Cat. No.: HY-K0306
Manual COA SDS Technical Support

MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

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2 mL In-stock
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    MedChemExpress Validation
    CD3+ cells were sorted from mouse splenocytes using the Mouse CD3+ T Cells Negative Selection Kit (HY-K0306). CD3+ T cells were isolated from C57BL/6 mouse splenocytes and stained with FITC-conjugated anti-mouse CD3 antibody (clone 145-2C11) before and after sorting for flow cytometric analysis. The results showed that the purity of CD3+ T cells increased from 28.9% (pre-sorting) to 98.5% (post-sorting).

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells and lymph nodes. Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3+ T cells.

    The kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

    Features of MCE Mouse CD3+ T Cells Negative Selection Kit

    •   Efficiency: Enables sorting of target cells in as little as 15 minutes;

    •   Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

    •   High Purity: Can yield sorted cells with a purity exceeding 95%;

    •   High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.
    Storage

    4°C，2 years

    Do not freeze the magnetic beads
    Components
    Components HY-K0306-1 mL (For 5 × 108 cells) HY-K0306-2 mL (For 109 cells)
    Biotin-Antibody Mix 100 μL 200 μL
    Streptavidin Magnetic Beads 1 mL 1 mL × 2
    Documentation

    Manual (436 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    mouse CD3+ T Cell Kitmouse CD3+ T Cell Sorting Magnetic BeadsCD3+ T cell Microbeads

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