1. MCE Kits
  2. Cell Isolation
  3. Mouse CD3+ T Cells Negative Selection Kit

Mouse CD3+ T Cells Negative Selection Kit 

Cat. No.: HY-K0306
Manual SDS

MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells, lymph nodes or other tissues.

Mouse CD3+ T Cells Negative Selection Kit
Size Price Stock Quantity
1 mL USD 580 In-stock
2 mL USD 965 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells, lymphnodes or other tissues. Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3+ T cells.

The kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

Features of MCE Mouse CD3+ T Cells Negative Selection Kit

Efficiency: Enables sorting of target cells in as little as 15 minutes;

Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

High Purity: Can yield sorted cells with a purity exceeding 97%;

High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.

Storage

4ºC,2 years

Do not freeze the magnetic beads

Components
Components HY-K0306-1 mL (For 5 × 108 cells) HY-K0306-2 mL (For 109 cells)
Biotin-Antibody Mix 100 μL 200 μL
Streptavidin Magnetic Beads 1 mL 1 mL × 2
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Mouse CD3+ T Cells Negative Selection Kit
Cat. No.:
HY-K0306
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