2. MCE Kits
  3. Cell Biology
  4. Cell Isolation
  5. Human CD3+ T Cells Negative Selection Kit

Human CD3+ T Cells Negative Selection Kit 

Cat. No.: HY-K0304
Manual COA SDS Technical Support

MCE Human CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from human peripheral blood mononuclear cells (PBMC).

Size Price Stock Quantity
200 μL In-stock
2 mL In-stock
or Bulk Inquiry

* Please select Quantity before adding items.

    MedChemExpress Validation
    Use the Human CD3+ T Cells Negative Selection Kit (HY-K0304) to sort CD3+ T cells from peripheral blood mononuclear cells isolated from human whole blood. CD3+ T cells were isolated from human PBMCs. Cells before and after sorting were stained with PE-conjugated anti-human CD3 antibody (clone OKT3) for flow cytometric analysis. The results showed that the purity of CD3+ T cells increased from 49.1% (pre-sorting) to 96.9% (post-sorting).

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE Human CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from human peripheral blood mononuclear cells (PBMC). Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3+ T cells.

    MCE Human CD3+ T Cells Negative Selection Kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

    Features of MCE Human CD3+ T Cells Negative Selection Kit

    •   Efficiency: Enables sorting of target cells in as little as 15 minutes;

    •   Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

    •   High Purity: Can yield sorted cells with a purity exceeding 97%;

    •   High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.
    Storage

    4°C，2 years

    Do not freeze the magnetic beads
    Components
    Components HY-K0304-0.2 mL (For 108 cells) HY-K0304-2 mL (For 109 cells)
    Biotin-Antibody Mix 20 μL 200 μL
    Streptavidin Magnetic Beads 0.2 mL 1 mL × 2
    Documentation

    Manual (106 KB) SDS (86 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    human CD3+ T Cell Kithuman CD3+ T Cell Sorting Magnetic BeadsCD3+ T cell Microbeads

    Your Recently Viewed Products:

    Bulk Inquiry

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Human CD3+ T Cells Negative Selection Kit
    Cat. No.:
    HY-K0304
    Quantity:
    MCE Japan Authorized Agent:

    Request for COA Report

    Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

    .

    Product Name

    		  

    Lot # *

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Remarks

    Request for COA Report

    We have received your request and will respond to you as soon as possible.