1. MCE Kits
  2. Cell Biology
  3. Cell Isolation
  4. Human CD3+ T Cells Negative Selection Kit

Human CD3+ T Cells Negative Selection Kit 

Cat. No.: HY-K0304
Manual SDS

MCE Human CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from human peripheral blood mononuclear cells (PBMC).

Human CD3+ T Cells Negative Selection Kit
Size Price Stock Quantity
200 μL USD 62 In-stock
2 mL USD 544 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Human CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from human peripheral blood mononuclear cells (PBMC). Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3+ T cells.

MCE Human CD3+ T Cells Negative Selection Kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

Features of MCE Human CD3+ T Cells Negative Selection Kit

Efficiency: Enables sorting of target cells in as little as 15 minutes;

文Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

High Purity: Can yield sorted cells with a purity exceeding 97%;

High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.

Storage

4ºC,2 years

Do not freeze the magnetic beads

Components
Components HY-K0304-0.2 mL (For 108 cells) HY-K0304-2 mL (For 109 cells)
Biotin-Antibody Mix 20 μL 200 μL
Streptavidin Magnetic Beads 0.2 mL 1 mL × 2
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Bulk Inquiry

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Human CD3+ T Cells Negative Selection Kit
Cat. No.:
HY-K0304
Quantity:
MCE Japan Authorized Agent: