Proteinase K (NGS grade)  (Synonyms: Protease K (NGS grade))

Proteinase K (Protease K) (NGS grade) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K (NGS grade) is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K (NGS grade) can be use for promoting methods of viral nucleic acid extraction, and detection. This product is NGS grade, no Nickase residue, and nucleic acid residue ≤5 pg/mg^[1].

Product Information
Source: Tritirachium album Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 7.81
Optimal pH: 7.0-12.0
Optimal temperature: 65°C
pH stability: pH 4.5-12.5 (25°C, 16 hours)
Thermal stability: Stable below 50°C (pH 8.0, 30 min)
Storage stability: Viability exceeds 90% within one year at 25°C
Activators: SDS (HY-K1030), Urea (HY-Y0271)
Inhibitors: Diisopropyl fluorophosphate (DFP), PMSF (HY-B0496)

Instructions
It can be dissolved in water at a concentration of 1mg/mL, or it can be dissolved according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

39450-01-6

Solid

White to off-white

[Proteinase K (NGS grade)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. W Ebeling, et al. Proteinase K From Tritirachium Album Limber. Eur J Biochem . 1974 Aug 15;47(1):91-7.  [Content Brief]