1. Metabolic Enzyme/Protease
  2. Ser/Thr Protease
  3. Proteinase K

Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection.

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Proteinase K Chemical Structure

Proteinase K Chemical Structure

CAS No. : 39450-01-6

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Description

Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection[1][2][3][4].

In Vitro

Proteinase K enzyme can be used to break amid bond between MTX and BSA and also amidic bonds in BSA structure[2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Proteinase K with superior protein-degrading property, can be used to detect the SARS-COV2 viral component directly from inoculated viral transport medium (VTM) without RNA extraction[3].
Protocol of rRTPCR detection of SARS-CoV2[3]:
1.Separate VTM of each SARS-COV2 samples into three EP tubes (45 μL per tube);
2.Add 2.5 µL (20 mg/mL concentrated) proteinase K (PK), making a final concentration of PK ~1 mg/ml VTM;
3.Incubate at 25 ℃ for 5 min;
4.Incubate at 60 ℃ for 15 min;
5.Incubate at 98 ℃ for 10 min, and store samples at 4 ℃;
6.Use 5 µL of extracted RNA as template to execute rRTPCR assay;
7.Exert rRTPCR detection by BIORAD CFX96™ Real-Time Thermocycler, with a positive standard of threshold cycle (Ct) values less than 40.
Proteinase K improves the homogenization effects and positive rates of sputum samples, and increases nucleic acid concentration extracted from it[4].
Protocol of nucleic acid extraction of influenza A virus (IAV)[4]:
1.Separate specimen into three EP tubes (500 μL per tube);
2.Add 500 μL 0.4 mg/mL Proteinase K buffer, vortex for 20 seconds;
3.Incubate at 55 ℃ for 25 min, vortex 5 seconds every 5 min;
4.Extract RNA by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China) with 200 μL samples;
5.Evaluate A260/A280 ratio of pure RNA products, with a standard ranged from 1.8 to 2.0.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Proteinase K]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (Need ultrasonic)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (Infinity mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Proteinase K
Cat. No.:
HY-108717
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