Guanosine triphosphate trisodium salt hydrate
Based on 9 publication(s) in Google Scholar
Guanosine triphosphate (GTP) trisodium salt hydrate is a critical nucleotide and regulator of cellular metabolism. Guanosine triphosphate trisodium salt hydrate promotes ribosomal DNA localization, pre-rRNA transcription and ribosome biogenesis by binding to RNA polymerase I and GPN proteins (GPN1/3). Guanosine triphosphate trisodium salt hydrate links MYC-dependent ribosome biogenesis to nucleotide sufficiency, acts as a metabolic gatekeeper supporting protein synthesis, DNA/RNA synthesis and cellular signal transduction, while also participating in the physiological activities of pancreatic β-cells and serving as an oxidative substrate for reactive oxygen species. In small cell lung cancer with high MYC expression, Guanosine triphosphate trisodium salt hydrate accumulates through the IMPDH-driven synthetic pathway, thereby affecting apoptosis and mitotic processes. Guanosine triphosphate trisodium salt hydrate is used in the research of small cell lung cancer, hepatoblastoma and cellular metabolism.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 207300-85-4
- Formula: C10H13N5Na3O14P3·xH2O
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Storage:
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Guanosine triphosphate trisodium salt hydrate
More- Cancer Commun (Lond). 2025 May 29. [Abstract]
- Mol Cell. 2022 Jun 2;82(11):2032-2049.e7. [Abstract]
- Acta Pharm Sin B. 2024 Apr;14(4):1864-1877. [Abstract]
- Anal Chim Acta. 2021 Dec 15:1188:339180. [Abstract]
- J Mol Biol. 2024 Apr 15;436(8):168513. [Abstract]
- J Biol Chem. 2026 Jan 28:111214. [Abstract]
- Bioorg Med Chem. 2021 Jan 1:29:115899. [Abstract]
- STAR Protoc. 2026 Mar 20;7(1):104353. [Abstract]
- bioRxiv. 2024 Apr 24.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
Description
IC50 & Target
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RNA Polymerase |
Human Endogenous Metabolite |
In Vitro
Primary MYC-high human small-cell lung cancer tumors have elevated endogenous guanosine triphosphate levels independent of proliferation status[1].
Guanosine triphosphate (GTP depletion via 1 μM MPA; 12 h) reduced protein synthesis in chemoresistant DMS53-CR human small-cell lung cancer cells, while restoring guanosine triphosphate levels (20 μM guanosine; 12 h) rescued protein synthesis activity[1].
Guanosine triphosphate binding to MYC-upregulated GPN1 and GPN3 GTPases is required for RNA polymerase I localization, ribosome biogenesis, and proliferation in MYC-high H82 human small-cell lung cancer cells, and constitutively GTP-bound GPN1/GPN3 mutants protect cells from guanosine triphosphate depletion-mediated inhibition of these activities[1].
Depletion of cellular GTP (1 μg/ml mycophenolic acid; 1-24 h) in HIT-T15 insulin-secreting β-cells potently inhibits mitogenesis, with significant inhibition observed as early as 1 hour and near-complete inhibition after 6-24 hours of treatment[2].
Depletion of cellular GTP (1.6-6.3 μg/ml mycophenolic acid; 18 h) in HIT-T15 and INS-1 insulin-secreting β-cells inhibits Ca2+-stimulated insulin secretion[2].
Guanosine triphosphate (1 mM; 4 h at 37 °C) undergoes oxidation to oxo8GTP in the presence of a ROS-generating system (1 mM L-ascorbic acid + 10 μM cupric sulfate), with oxo8GTP levels increasing ~4-fold and GTP levels showing a small significant decrease relative to control[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HIT-T15 insulin-secreting β-cells, INS-1 insulin-secreting β-cells
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Concentration:0.3-10 μg/ml mycophenolic acid (HIT-T15 cells, GTP depleted to apoptosis-inducing levels); 25 μg/ml mycophenolic acid (INS-1 cells, GTP depleted to apoptosis-inducing levels)
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Incubation Time:24-96 h (HIT-T15 cells); 48 h (INS-1 cells)
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Result:Reduced HIT-T15 cell number by 70% after 48 hours of 3 μg/ml mycophenolic acid treatment, with parallel reductions in DNA, protein, and insulin content.
Reduced MTS formazan production by 30% (0.3 μg/ml, 48 h) to 80% (1 μg/ml, 96 h) in HIT-T15 cells. Induced apoptotic nuclear changes in 29% (1 μg/ml), 49% (3 μg/ml), and 70% (10 μg/ml) of HIT-T15 cells after 48 hours of treatment.
Increased oligonucleosome enrichment factor to ~3.5-fold of control in HIT-T15 cells after 48 hours of treatment.
Revealed chromatin condensation/margination in 25% and apoptotic bodies in 16% of HIT-T15 cells via electron microscopy after 48 hours of treatment.
Increased oligonucleosome enrichment factor to ~3-fold of control and induced apoptotic nuclear changes in INS-1 cells after 48 hours of 25 μg/ml mycophenolic acid treatment.
Chemical Information
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CAS No. 207300-85-4
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Appearance Solid
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Formula C10H13N5Na3O14P3·xH2O
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Color White to off-white
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SMILES
O=C1C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O3)=C2NC(N)=N1.[xH2O]
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Synonyms
GTP trisodium salt hydrate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (9)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma. [Abstract]2025 May 29. PMID: 40443016 -
Mol Cell
Cytoplasmic PARP1 links the genome instability to the inhibition of antiviral immunity through PARylating cGAS. [Abstract]2022 Jun 2;82(11):2032-2049.e7. PMID: 35460603 -
Acta Pharm Sin B
Super-resolution imaging for in situ monitoring sub-cellular micro-dynamics of small molecule drug. [Abstract]2024 Apr;14(4):1864-1877. PMID: 38572114 -
Anal Chim Acta
2021 Dec 15:1188:339180. PMID: 34794559 -
J Mol Biol
2024 Apr 15;436(8):168513. PMID: 38447889 -
J Biol Chem
Allosteric targeting with antiviral nucleotide analogs allows fine-tuning of SAMHD1 dNTPase activity. [Abstract]2026 Jan 28:111214. PMID: 41617034
Guanosine triphosphate trisodium salt hydrate purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214. [Abstract]
Guanosine 5'-triphosphate trisodium (GTP) (0-100 μM) induced the highest maximum SAMHD1 dNTPase activity.
Guanosine triphosphate trisodium salt hydrate purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214. [Abstract]
Guanosine 5'-triphosphate trisodium (GTP) (5-1200 μM) effectively induced tetramerization of the recombinant SAMHD1 protein in a dose-dependent manner.
Guanosine triphosphate trisodium salt hydrate purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214. [Abstract]
High-confidence fits were obtained for the Guanosine 5'-triphosphate trisodium (GTP) (250 μM; 25 min)-activated hydrolysis of dATP.
Guanosine triphosphate trisodium salt hydrate purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214. [Abstract]
In the absence of competitors, increasing Guanosine 5'-triphosphate trisodium (GTP) (0-50 μM) preincubation concentrations led to increasing activity. Guanosine 5'-triphosphate trisodium (GTP) added at the reaction start further increased SAMHD1 activity.
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Bioorg Med Chem
Synthesis and biological evaluation of all possible inosine-mixed cyclic dinucleotides that activate different hSTING variants. [Abstract]2021 Jan 1:29:115899. PMID: 33285409 -
STAR Protoc
Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei. [Abstract]2026 Mar 20;7(1):104353. PMID: 41671092 -
Solvent & Solubility
In Vitro:
H2O : ≥ 150 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Huang F, et al. Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer. J Clin Invest. 2021;131(1):e139929. [Content Brief]
[2]. Li G, et al. Prolonged depletion of guanosine triphosphate induces death of insulin-secreting cells by apoptosis. Endocrinology. 1998;139(9):3752-3762. [Content Brief]
[3]. Bolin C, et al. Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;856(1-2):121-130. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Guanosine triphosphate trisodium salt hydrate Related Classifications
HY-12695B Related Classifications
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