Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

  • STAR Protoc. 2026 Mar 20;7(1):104353. doi: 10.1016/j.xpro.2026.104353.
Binbin Cui  1 ,  Xiayu Chen  2 ,  Mingfang Wang  2 ,  Yinyue Deng  3
Affiliations
  • 1. Pharmacy Department, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo 315046, China; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
  • 2. School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
  • 3. School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China. Electronic address: [email protected].
PMID: 41671092 DOI: 10.1016/j.xpro.2026.104353
Abstract

Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei. Technical assays include microscale thermophoresis (MST), quantitative reverse-transcription PCR (RT-qPCR), ultra-high-performance liquid chromatography-mass spectrometry (LC-MS), electrophoretic mobility shift assay (EMSA), bacterial Adenylate Cyclase two-hybrid (BACTH) assay, and molecular docking. For complete details on the use and execution of this protocol, please refer to Wang et al.1.

Keywords
Health sciences; Microbiology; Molecular biology.
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