2. Cytoskeleton Metabolic Enzyme/Protease Epigenetics
  3. Exosomes Endogenous Metabolite MicroRNA
  4. Guanosine-5'-triphosphate disodium salt

Guanosine-5'-triphosphate disodium salt  (Synonyms: 5'-GTP disodium salt)

Cat. No.: HY-W010737 Purity: 99.58%
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Guanosine 5'-triphosphate (5'-GTP) trisodium salt is a G protein (G proteins) signaling activator and a high-energy precursor in the biosynthesis of nucleotide units in DNA and RNA. Guanosine 5'-triphosphate trisodium salt can promote myogenic cell differentiation by upregulating miRNA (miR133a, miR133b) and myogenic regulatory factor expression, and by inducing human myogenic precursor cells to release exosomes containing guanosine molecules. Guanosine-5'-triphosphate disodium salt holds promise for research in biosynthesis and skeletal muscle regeneration.

For research use only. We do not sell to patients.

CAS No. : 56001-37-7

Size Price Stock Quantity
Solvent
250 mg (100 mM * 4.41 mL in Water) In-stock
Solvent
500 mg (100 mM * 8.82 mL in Water) In-stock
Solvent
1 g (100 mM * 17.63 mL in Water) In-stock

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Guanosine-5'-triphosphate disodium salt:

Guanosine-5'-triphosphate disodium salt Related Antibodies

Top Publications Citing Use of Products

    Guanosine-5'-triphosphate disodium salt purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214.

    Guanosine 5'-triphosphate trisodium (GTP) (0-100 μM) induced the highest maximum SAMHD1 dNTPase activity.

    Guanosine-5'-triphosphate disodium salt purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214.

    Guanosine 5'-triphosphate trisodium (GTP) (5-1200 μM) effectively induced tetramerization of the recombinant SAMHD1 protein in a dose-dependent manner.

    Guanosine-5'-triphosphate disodium salt purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214.

    High-confidence fits were obtained for the Guanosine 5'-triphosphate trisodium (GTP) (250 μM; 25 min)-activated hydrolysis of dATP.

    Guanosine-5'-triphosphate disodium salt purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 28:111214.

    In the absence of competitors, increasing Guanosine 5'-triphosphate trisodium (GTP) (0-50 μM) preincubation concentrations led to increasing activity. Guanosine 5'-triphosphate trisodium (GTP) added at the reaction start further increased SAMHD1 activity.

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    Human Endogenous Metabolite Microbial Metabolite

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    Description

    Guanosine 5'-triphosphate (5'-GTP) trisodium salt is a G protein (G proteins) signaling activator and a high-energy precursor in the biosynthesis of nucleotide units in DNA and RNA. Guanosine 5'-triphosphate trisodium salt can promote myogenic cell differentiation by upregulating miRNA (miR133a, miR133b) and myogenic regulatory factor expression, and by inducing human myogenic precursor cells to release exosomes containing guanosine molecules. Guanosine-5'-triphosphate disodium salt holds promise for research in biosynthesis and skeletal muscle regeneration[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Guanosine-5'-triphosphate disodium salt (500 μM, 24 hours) significantly promoted the upregulation of miR133a, miR133b, and myogenic regulatory factors Pax7, MyoD, and Myogenin in differentiated human myogenic precursor cells (MPCs)[2].
    Guanosine-5'-triphosphate disodium salt (5'-GTP trisodium salt) (500 μM, 24 h) can induce the release of exosomes from human myogenic progenitor cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Guanosine-5'-triphosphate disodium salt Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Human myogenic precursor cells (MPCs)
    Concentration: 500 μM
    Incubation Time: 24 h
    Result: led to a significant upregulation of miR133a and miR133b and induced human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu.
    Molecular Weight

    567.14

    Formula

    C10H14N5Na2O14P3
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    O=C1C2=C(N(C=N2)[C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O[Na])=O)=O)(O[Na])=O)O3)N=C(N)N1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, protect from light, 2 years
    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (220.40 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7632 mL 8.8162 mL 17.6323 mL
    5 mM 0.3526 mL 1.7632 mL 3.5265 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.58%

    SDS (394 KB)

    COA (250 KB) HNMR (285 KB) CNMR (286 KB) RP-HPLC (205 KB) MS (80 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.7632 mL 8.8162 mL 17.6323 mL 44.0808 mL
    5 mM 0.3526 mL 1.7632 mL 3.5265 mL 8.8162 mL
    10 mM 0.1763 mL 0.8816 mL 1.7632 mL 4.4081 mL
    15 mM 0.1175 mL 0.5877 mL 1.1755 mL 2.9387 mL
    20 mM 0.0882 mL 0.4408 mL 0.8816 mL 2.2040 mL
    25 mM 0.0705 mL 0.3526 mL 0.7053 mL 1.7632 mL
    30 mM 0.0588 mL 0.2939 mL 0.5877 mL 1.4694 mL
    40 mM 0.0441 mL 0.2204 mL 0.4408 mL 1.1020 mL
    50 mM 0.0353 mL 0.1763 mL 0.3526 mL 0.8816 mL
    60 mM 0.0294 mL 0.1469 mL 0.2939 mL 0.7347 mL
    80 mM 0.0220 mL 0.1102 mL 0.2204 mL 0.5510 mL
    100 mM 0.0176 mL 0.0882 mL 0.1763 mL 0.4408 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Guanosine-5'-triphosphate disodium salt Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Guanosine-5'-triphosphate disodium56001-37-75'-GTP disodiumExosomesEndogenous MetaboliteMicroRNAmiRNAHuman myogenic precursor cells (MPCs)Proliferative functionInhibitorinhibitorinhibit

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