Guanosine 5'-diphosphate disodium salt
Based on 2 publication(s) in Google Scholar
Guanosine 5'-diphosphate (GDP) disodium salt, a purine nucleoside diphosphate, is interconverted to guanosine by the action of exonucleotidase and phosphorylation of nucleoside to guanine. Guanosine 5'-diphosphate disodium salt activates adenosine 5'-triphosphate-sensitive K+ channel and is used to study the kinetics and characteristics of GTPases such as those associated with G-protein coupled receptors (GPCR). Guanosine 5'-diphosphate disodium salt is a potential iron mobilizer, which prevents the Hepcidin (HY-P70400)-ferroportin interaction and modulates the interleukin-6 (IL-6)/stat-3 pathway. Elevated levels of guanosine 5’-diphosphate are associated with the pathogenesis of neurological diseases. Guanosine 5'-diphosphate disodium salt is promising for the research of inflammation, such as anemia of inflammation (AI).
For research use only. We do not sell to patients.
- Purity : 96.42%
- CAS No.: 7415-69-2
- Formula: C10H13N5Na2O11P2
- Molecular Weight:487.16
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-W010737
Guanosine triphosphate disodium -
HY-W010918
Adenosine 5'-diphosphate -
HY-A0181
Adenosine monophosphate -
HY-N5134
5'-Guanylic acid -
HY-B0345A
ATP disodium salt -
HY-12695
Guanosine triphosphate trisodium -
HY-101981
Uridine 5'-monophosphate -
HY-W009162
Cytidine 5'-monophosphate -
HY-A0181R
Adenosine monophosphate (Standard) -
HY-W010832
Uridine-5'-diphosphate disodium salt -
HY-W013093
Uridine triphosphate trisodium salt -
HY-W010970
5'-Guanylic acid disodium salt
Publications Citing Use of MedChemExpress (MCE) Guanosine 5'-diphosphate disodium salt
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Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
[2]|
Human Endogenous Metabolite |
In Vitro
Guanosine 5'-diphosphate disodium salt (10 μM, 24 h) prevents Hepcidin (HY-P70400)-induced ferroportin (FPN) internalization with effective cellular iron efflux in HepG2 and Caco-2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2, Caco-2 cell
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Concentration:10 μM
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Incubation Time:24 h
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Result:Increased FPN protein level whereas, ferritin protein levels were significantly decreased compared to control.
In Vivo
Guanosine 5'-diphosphate disodium salt (30 mL/kg, i.p., three times a week for two weeks) can improve turpentine-induced inflammatory anemia in mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice[2].
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Dosage:30 mL/kg
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Administration:Intraperitoneal injection (i.p.)
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Result:Increased hemoglobin levels and serum iron levels in the long-term dosing model.
Decreased Hamp mRNA expression, and spleen iron content was reduced by 46% in the short-term dosing model.
Chemical Information
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CAS No. 7415-69-2
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Appearance Solid
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Molecular Weight 487.16
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Formula C10H13N5Na2O11P2
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(N=C(N)NC3=O)=C3N=C2)O[C@@H]1COP(OP(O)([O-])=O)([O-])=O.[2Na+]
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Synonyms
GDP disodium salt
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
miR-143-null Is against Diet-Induced Obesity by Promoting BAT Thermogenesis and Inhibiting WAT Adipogenesis. [Abstract]2022 Oct 27;23(21):13058. PMID: 36361843
Guanosine 5'-diphosphate disodium salt purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 27;23(21):13058. [Abstract]
Guanosine diphosphate (2 mM) inhibited the oxygen consumption of mitochondria isolated from BAT of WT and miR-143 KO mice fed HFD for 1 week.
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Endocrinology
MiR-143 targets SYK to regulate NEFA uptake and contribute to thermogenesis in male mice. [Abstract]2023 Aug 1;164(9):bqad114. PMID: 37486737
Guanosine 5'-diphosphate disodium salt purchased from MedChemExpress. Usage Cited in: Endocrinology. 2023 Aug 1;164(9):bqad114. [Abstract]
The mitochondrial respiration of BAT (left) and scWAT (right) was significantly increased at both basal and substrate-induced states in MiR-143 KO mice, and this increase was diminished by guanosine diphosphate (2 mM).
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (205.27 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (205.27 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. S Kajioka, et al. Guanosine diphosphate activates an adenosine 5'-triphosphate-sensitive K+ channel in the rabbit portal vein. J Physiol. 1991 Dec;444:397-418. [Content Brief]
[2]. Angmo S, et al. Identification of Guanosine 5'-diphosphate as Potential Iron Mobilizer: Preventing the Hepcidin-Ferroportin Interaction and Modulating the Interleukin-6/Stat-3 Pathway. Sci Rep. 2017 Jan 5;7:40097. [Content Brief]
[3]. Ciruela F. Guanosine behind the scene. J Neurochem. 2013 Aug;126(4):425-7. [Content Brief]
[4]. Raj GV, et al. Guanosine phosphate binding protein coupled receptors in prostate cancer: a review. J Urol. 2002 Mar;167(3):1458-63. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.0527 mL | 10.2636 mL | 20.5271 mL | 51.3178 mL |
| 5 mM | 0.4105 mL | 2.0527 mL | 4.1054 mL | 10.2636 mL | |
| 10 mM | 0.2053 mL | 1.0264 mL | 2.0527 mL | 5.1318 mL | |
| 15 mM | 0.1368 mL | 0.6842 mL | 1.3685 mL | 3.4212 mL | |
| 20 mM | 0.1026 mL | 0.5132 mL | 1.0264 mL | 2.5659 mL | |
| 25 mM | 0.0821 mL | 0.4105 mL | 0.8211 mL | 2.0527 mL | |
| 30 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7106 mL | |
| 40 mM | 0.0513 mL | 0.2566 mL | 0.5132 mL | 1.2829 mL | |
| 50 mM | 0.0411 mL | 0.2053 mL | 0.4105 mL | 1.0264 mL | |
| 60 mM | 0.0342 mL | 0.1711 mL | 0.3421 mL | 0.8553 mL | |
| 80 mM | 0.0257 mL | 0.1283 mL | 0.2566 mL | 0.6415 mL | |
| 100 mM | 0.0205 mL | 0.1026 mL | 0.2053 mL | 0.5132 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Guanosine 5'-diphosphate disodium salt Related Classifications
HY-113066A Related Classifications
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