Guanosine 5'-diphosphate
Based on 2 publication(s) in Google Scholar
Guanosine 5'-diphosphate (GDP) is a nucleoside diphosphate that activates adenosine 5'-triphosphate-sensitive K+ channel. Guanosine 5'-diphosphate is an iron mobilizer, which forms a complex with hepcidin to inhibit the hepcidin-ferroportin (FPN) interaction and modulates the IL-6/stat-3 pathway. Guanosine 5'-diphosphate ameliorates the Turpentine-induced anemia of inflammation (AI) in mice when combined with FeSO4. Guanosine 5'-diphosphate can be used in the research of AI.
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- CAS No.: 146-91-8
- 화학식: C10H15N5O11P2
- 분자량:443.20
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) Guanosine 5'-diphosphate
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
제품 설명
IC50 & Target
[2]|
Human Endogenous Metabolite |
IL-6 |
Stat-3 |
In Vitro
Guanosine 5'-diphosphate (100 μM-1 mM) can activate the ATP-sensitive K+ channel in the rabbit portal vein, in the presence of Pinacidil (HY-14290)[1].
Guanosine 5'-diphosphate (10 μM, 24 h) inhibits hepcidin-induced FPN internalization in FPN-GFP cell lines (HEK293T) and antagonizes hepcidin to increase the FPN-mediated cellular iron efflux in HepG2 and Caco-2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Guanosine 5'-diphosphate (30 mg/kg, i.p., every 24 hours for 2 weeks) ameliorates the Turpentine-induced AI in mice when combined with FeSO4 (2 mg/kg)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice (25 ± 3 g) injected once a week with Turpentine for 2 week[2]
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Dosage:30 mg/kg
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Administration:i.p., every 24 hours for 2 weeks
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Result:Significantly increased haemoglobin level when combined with FeSO4.
Significant increased serum iron level when combined with FeSO4.
Reversed hepcidin-mediated FPN internalization with increased iron accumulation with decreased liver and spleen iron level, when combined with FeSO4.
Increased FPN level (liver, Spleen, and enterocyte) and reduced iron storage ferritin level (spleen and enterocyte) for effective iron-mediated erythropoiesis thus improving hypoferremia.
Significantly decreased Hamp mRNA expression and hepcidin expression at transcriptional level, when combined with FeSO4.
Reduced Stat-3 phosphorylation in mice hepatocyte.
Chemical Information
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CAS No. 146-91-8
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분자량 443.20
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화학식 C10H15N5O11P2
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SMILES
O=P(O)(OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C2N=C(N)NC3=O)O1)(O)=O)O
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Synonyms
GDP
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Structure Classification
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
miR-143-null Is against Diet-Induced Obesity by Promoting BAT Thermogenesis and Inhibiting WAT Adipogenesis. [Abstract]2022 Oct 27;23(21):13058. PMID: 36361843
Guanosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 27;23(21):13058. [Abstract]
Guanosine diphosphate (2 mM) inhibited the oxygen consumption of mitochondria isolated from BAT of WT and miR-143 KO mice fed HFD for 1 week.
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Endocrinology
MiR-143 targets SYK to regulate NEFA uptake and contribute to thermogenesis in male mice. [Abstract]2023 Aug 1;164(9):bqad114. PMID: 37486737
Guanosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Endocrinology. 2023 Aug 1;164(9):bqad114. [Abstract]
The mitochondrial respiration of BAT (left) and scWAT (right) was significantly increased at both basal and substrate-induced states in MiR-143 KO mice, and this increase was diminished by guanosine diphosphate (2 mM).
Protocol
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
순도&문서
References
[1]. S Kajioka, et al. Guanosine diphosphate activates an adenosine 5'-triphosphate-sensitive K+ channel in the rabbit portal vein. J Physiol. 1991 Dec;444:397-418. [Content Brief]
[2]. Angmo S, et al. Identification of Guanosine 5'-diphosphate as Potential Iron Mobilizer: Preventing the Hepcidin-Ferroportin Interaction and Modulating the Interleukin-6/Stat-3 Pathway. Sci Rep. 2017 Jan 5;7:40097. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Guanosine 5'-diphosphate Related Classifications
HY-113066 Related Classifications
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