Adenosine 5'-diphosphate (Synonyms: Adenosine diphosphate; ADP)

Name: Adenosine 5'-diphosphate
Brand: MedChemExpress
SKU: HY-W010918
Rating: 5.0 (9 reviews)

Cat. No.: HY-W010918 Purity: 99.52%: Data Sheet
COA

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Adenosine 5'-diphosphate (Adenosine diphosphate) is a nucleoside diphosphate. Adenosine 5'-diphosphate is the product of ATP dephosphorylation by ATPases. Adenosine 5'-diphosphate induces human platelet aggregation and inhibits stimulated adenylate cyclase by an action at P_2T-purinoceptors.

For research use only. We do not sell to patients.

CAS No. : 58-64-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Adenosine 5'-diphosphate:

Adenosine 5'-diphosphate-¹⁵N₅ dilithium In-stock
Adenosine 5'-diphosphate disodium In-stock
Adenosine 5'-diphosphate sodium salt In-stock
Adenosine 5'-diphosphate (Standard) In-stock
Adenosine 5'-diphosphate-¹³C₅ Get quote

9 Publications Citing Use of MCE Adenosine 5'-diphosphate

Flow Cytometry

Others

: Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157553. [Abstract]; Flow cytometry analysis of CD61 and CD62P expression in ADP (20 μM; 10 min) -stimulated platelets after glycyrrhizin treatment.

: Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157553. [Abstract]; Quantitative analysis of the adhesion thickness of platelets on fibrinogen-coated surfaces after ADP (20 μM) activation, with or without glycyrrhizin treatment.

: Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Jun 8:74:103225. [Abstract]; ADP (5 mM; 1 h). Representative Western blots show the protein levels of p-PI3K, PI3K, p-AKT, AKT, p-NRF2, NRF2, GSDMD, and GSDMD-N in different groups.

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Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
Cerebral cortex neuron	EC₅₀	19 μM Compound: 2	Neuroprotective activity against Fe2+-induced oxidative damage in rat primary cortical neurons assessed as cell viability after 24 hrs by MTT assay Neuroprotective activity against Fe2+-induced oxidative damage in rat primary cortical neurons assessed as cell viability after 24 hrs by MTT assay	[PMID: 26447940]
Oocyte	EC₅₀	28000 nM Compound: ADP	Antagonist activity against recombinant rat P2X purinoceptor 4 (P2X4) at 30 uM, expressed in Xenopus oocytes Antagonist activity against recombinant rat P2X purinoceptor 4 (P2X4) at 30 uM, expressed in Xenopus oocytes	[PMID: 12213051]
PC-12	IC₅₀	21 μM Compound: 2	Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis	[PMID: 26447940]
PC-12	IC₅₀	21 μM Compound: 2, ADP	Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis	[PMID: 23751098]

In Vitro

Adenosine 5'-diphosphate consists of the pyrophosphate group, the pentose sugar ribose, and the nucleobase adenine. Adenosine 5'-diphosphate is the product of ATP dephosphorylation by ATPases. ADP is converted back to ATP by ATP synthases. ATP is an important energy transfer molecule in cells. Adenosine 5'-diphosphate is utilized in a wide number of cellular processes, including respiration, biosynthetic reactions, motility, and cell division.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

427.20

Formula

C₁₀H₁₅N₅O₁₀P₂

CAS No.

58-64-0

Appearance

Solid

Color

White to off-white

SMILES

O=P(O)(OP(O)(OC[C@H]1O[C@@H](N2C=NC3=C(N)N=CN=C23)[C@H](O)[C@@H]1O)=O)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (117.04 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3408 mL	11.7041 mL	23.4082 mL
5 mM	0.4682 mL	2.3408 mL	4.6816 mL
10 mM	0.2341 mL	1.1704 mL	2.3408 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (234.08 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.52%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (252 KB) HNMR (274 KB) RP-HPLC (275 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Arts IC, et al. Adenosine 5'-triphosphate (ATP) supplements are not orally bioavailable: a randomized, placebo-controlled cross-over trial in healthy humans. J Int Soc Sports Nutr. 2012 Apr 17;9(1):16. [Content Brief]

[2]. Noel J. Cusack, et al. Effects of phosphate-modified analogs of adenosine 5′-diphosphate and adenosine 5′‐triphosphate at P_2T-purinoceptors mediating human platelet activation by ADP. April 1996.

[3]. Adenosine-5'-diphosphate

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.3408 mL	11.7041 mL	23.4082 mL	58.5206 mL
	5 mM	0.4682 mL	2.3408 mL	4.6816 mL	11.7041 mL
	10 mM	0.2341 mL	1.1704 mL	2.3408 mL	5.8521 mL
	15 mM	0.1561 mL	0.7803 mL	1.5605 mL	3.9014 mL
	20 mM	0.1170 mL	0.5852 mL	1.1704 mL	2.9260 mL
	25 mM	0.0936 mL	0.4682 mL	0.9363 mL	2.3408 mL
	30 mM	0.0780 mL	0.3901 mL	0.7803 mL	1.9507 mL
	40 mM	0.0585 mL	0.2926 mL	0.5852 mL	1.4630 mL
	50 mM	0.0468 mL	0.2341 mL	0.4682 mL	1.1704 mL
	60 mM	0.0390 mL	0.1951 mL	0.3901 mL	0.9753 mL
	80 mM	0.0293 mL	0.1463 mL	0.2926 mL	0.7315 mL
	100 mM	0.0234 mL	0.1170 mL	0.2341 mL	0.5852 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Adenosine 5'-diphosphate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Adenosine 5'-diphosphate58-64-0Adenosine diphosphate ADPEndogenous MetaboliteInhibitorinhibitorinhibit

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Adenosine 5'-diphosphate (Synonyms: Adenosine diphosphate; ADP)

Customer Review

Other Forms of Adenosine 5'-diphosphate:

Adenosine 5'-diphosphate Related Antibodies

9 Publications Citing Use of MCE Adenosine 5'-diphosphate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Adenosine 5'-diphosphate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Adenosine 5'-diphosphate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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