Adenosine 5'-diphosphate
Based on 9 publication(s) in Google Scholar
Adenosine 5'-diphosphate (Adenosine diphosphate) is a nucleoside diphosphate. Adenosine 5'-diphosphate is the product of ATP dephosphorylation by ATPases. Adenosine 5'-diphosphate induces human platelet aggregation and inhibits stimulated adenylate cyclase by an action at P2T-purinoceptors.
For research use only. We do not sell to patients.
- Purity: 99.52%
- CAS No.: 58-64-0
- Formula: C10H15N5O10P2
- Molecular Weight:427.20
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications Citing Use of MedChemExpress (MCE) Adenosine 5'-diphosphate
More- Nature. 2026 Jan;649(8096):423-431. [Abstract]
- Adv Mater. 2025 Apr;37(17):e2416349. [Abstract]
- Acta Pharm Sin B. 2025 Oct 24.
- Chem Eng J. 2026 Feb 12.
- Redox Biol. 2024 Jun 8:74:103225. [Abstract]
- Int J Biol Macromol. 2025 May 7:144068. [Abstract]
- Phytomedicine. 2025 Dec:149:157553. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Cell Biomater. 2026 Mar 6.
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Flow Cytometry
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Others
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WB
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Cerebral cortex neuron | EC50 |
19 μM
Compound: 2
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Neuroprotective activity against Fe2+-induced oxidative damage in rat primary cortical neurons assessed as cell viability after 24 hrs by MTT assay
Neuroprotective activity against Fe2+-induced oxidative damage in rat primary cortical neurons assessed as cell viability after 24 hrs by MTT assay
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[PMID: 26447940] |
| Oocyte | EC50 |
28 μM
Compound: ADP
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Antagonist activity against recombinant rat P2X purinoceptor 4 (P2X4) at 30 uM, expressed in Xenopus oocytes
Antagonist activity against recombinant rat P2X purinoceptor 4 (P2X4) at 30 uM, expressed in Xenopus oocytes
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[PMID: 12213051] |
| PC-12 | IC50 |
21 μM
Compound: 2, ADP
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Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis
Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced ROS production after 1 hr by DCFH-DA staining-based fluorometric analysis
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[PMID: 23751098] |
| PC-12 | IC50 |
21 μM
Compound: 2
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Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis
Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis
|
[PMID: 26447940] |
Adenosine 5'-diphosphate consists of the pyrophosphate group, the pentose sugar ribose, and the nucleobase adenine. Adenosine 5'-diphosphate is the product of ATP dephosphorylation by ATPases. ADP is converted back to ATP by ATP synthases. ATP is an important energy transfer molecule in cells. Adenosine 5'-diphosphate is utilized in a wide number of cellular processes, including respiration, biosynthetic reactions, motility, and cell division.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 58-64-0
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Appearance Solid
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Molecular Weight 427.20
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Formula C10H15N5O10P2
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Color White to off-white
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SMILES
O=P(O)(OP(O)(OC[C@H]1O[C@@H](N2C=NC3=C(N)N=CN=C23)[C@H](O)[C@@H]1O)=O)O
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Synonyms
Adenosine diphosphate; ADP
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jan;649(8096):423-431. PMID: 41193806 -
Adv Mater
2025 Apr;37(17):e2416349. PMID: 40025988 -
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Redox Biol
FAM3A plays a key role in protecting against tubular cell pyroptosis and acute kidney injury. [Abstract]2024 Jun 8:74:103225. PMID: 38875957
Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Jun 8:74:103225. [Abstract]
ADP (5 mM; 1 h). Representative Western blots show the protein levels of p-PI3K, PI3K, p-AKT, AKT, p-NRF2, NRF2, GSDMD, and GSDMD-N in different groups.
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Int J Biol Macromol
FKBP5 inhibitor suppresses platelet activation and thrombosis by inhibiting IKBKE/PI3K/Rap1 pathway. [Abstract]2025 May 7:144068. PMID: 40345291 -
Phytomedicine
Liquiritigenin attenuates thrombosis by inhibiting P2Y12R-mediated Src/PLCγ2 signaling and cytoplasmic Ca2+ mobilization in platelets. [Abstract]2025 Dec:149:157553. PMID: 41273866
Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157553. [Abstract]
Flow cytometry analysis of CD61 and CD62P expression in ADP (20 μM; 10 min) -stimulated platelets after glycyrrhizin treatment.
Adenosine 5'-diphosphate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157553. [Abstract]
Quantitative analysis of the adhesion thickness of platelets on fibrinogen-coated surfaces after ADP (20 μM) activation, with or without glycyrrhizin treatment.
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Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Solvent & Solubility
H2O : 50 mg/mL (117.04 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (234.08 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.3408 mL | 11.7041 mL | 23.4082 mL | 58.5206 mL |
| 5 mM | 0.4682 mL | 2.3408 mL | 4.6816 mL | 11.7041 mL | |
| 10 mM | 0.2341 mL | 1.1704 mL | 2.3408 mL | 5.8521 mL | |
| 15 mM | 0.1561 mL | 0.7803 mL | 1.5605 mL | 3.9014 mL | |
| 20 mM | 0.1170 mL | 0.5852 mL | 1.1704 mL | 2.9260 mL | |
| 25 mM | 0.0936 mL | 0.4682 mL | 0.9363 mL | 2.3408 mL | |
| 30 mM | 0.0780 mL | 0.3901 mL | 0.7803 mL | 1.9507 mL | |
| 40 mM | 0.0585 mL | 0.2926 mL | 0.5852 mL | 1.4630 mL | |
| 50 mM | 0.0468 mL | 0.2341 mL | 0.4682 mL | 1.1704 mL | |
| 60 mM | 0.0390 mL | 0.1951 mL | 0.3901 mL | 0.9753 mL | |
| 80 mM | 0.0293 mL | 0.1463 mL | 0.2926 mL | 0.7315 mL | |
| 100 mM | 0.0234 mL | 0.1170 mL | 0.2341 mL | 0.5852 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.