2. Metabolic Enzyme/Protease Immunology/Inflammation Anti-infection
  3. Endogenous Metabolite NOD-like Receptor (NLR) Interleukin Related Bacterial
  4. ATP disodium salt, 100 mM Solution, PCR Grade

ATP disodium salt, 100 mM Solution, PCR Grade  (Synonyms: Adenosine 5'-triphosphate disodium salt, 100 mM Solution, PCR Grade)

Cat. No.: HY-B2176I
COA
SDS
Handling Instructions Technical Support

ATP (Adenosine 5'-triphosphate) disodium salt, 100 mM Solution, PCR Grade is a central component of energy storage and metabolism in vivo. ATP disodium salt, 100 mM Solution, PCR Grade provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt, 100 mM Solution, PCR Grade is an important endogenous signaling molecule in immunity and inflammation. ATP disodium salt, 100 mM Solution, PCR Grade can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP disodium salt, 100 mM Solution, PCR Grade has anti-bacterial infection effects and can protect mice against bacterial infection in mice.

For research use only. We do not sell to patients.

CAS No. : 987-65-5

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Based on 143 publication(s) in Google Scholar

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Top Publications Citing Use of Products

143 Publications Citing Use of MCE ATP disodium salt, 100 mM Solution, PCR Grade

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Cell Proliferation/Viability Assay
RT-PCR

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1.

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt, 100 mM Solution, PCR Grade purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    View All NOD-like Receptor (NLR) Isoform Specific Products:

    View All Isoforms
    NLRP3 NLRP1 NOD1 NOD2

    View All Interleukin Related Isoform Specific Products:

    View All Isoforms
    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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    Description

    ATP (Adenosine 5'-triphosphate) disodium salt, 100 mM Solution, PCR Grade is a central component of energy storage and metabolism in vivo. ATP disodium salt, 100 mM Solution, PCR Grade provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt, 100 mM Solution, PCR Grade is an important endogenous signaling molecule in immunity and inflammation. ATP disodium salt, 100 mM Solution, PCR Grade can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP disodium salt, 100 mM Solution, PCR Grade has anti-bacterial infection effects and can protect mice against bacterial infection in mice[1][2][3][4].

    IC50 & Target[4]

    NLRP3 inflammasome

     

    IL-1β

     

    In Vitro

    ATP (5 mM; 1 hour) disodium salt, 100 mM Solution, PCR Grade co-treatment with LPS (1 μg/mL) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP (2 mM; 0.5-24 hours) disodium salt, 100 mM Solution, PCR Grade induces secretion of IL-1β, KC and MIP-2 from BMDMs in a caspase-1 activation-dependent manner[4].
    ATP disodium salt, 100 mM Solution, PCR Grade promotes neutrophil chemotaxis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP disodium salt, 100 mM Solution, PCR Grade Related Antibodies
    In Vivo

    ATP (50 mg/kg; i.p., 1-24 h before bacterial challenge) disodium salt, 100 mM Solution, PCR Grade protects mice against bacterial infection in mice, such as E. coli and S. aureus[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice infected with E. coli and S. aureus (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, 1, 4, 24 h before bacterial (E. coli) challenge
    Result: Reduced peritoneal bacterial counts.
    Increased the levels of IL-1β, KC and MIP-2 in the peritoneal lavage fluid.
    Recruited neutrophils to the peritoneum.
    Molecular Weight

    551.14

    Formula

    C10H14N5Na2O13P3
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    O=P(O[Na])(O[Na])OP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(N)=C3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Purity & Documentation

    SDS (252 KB)

    COA (251 KB)

    Handling Instructions (2659 KB)
    References
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    ATP disodium salt, 100 mM Solution, PCR Grade Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium salt, 100 mM Solution, PCR Grade987-65-5Adenosine 5'-triphosphate disodium salt, 100 mM Solution, PCR GradeEndogenous MetaboliteNOD-like Receptor (NLR)Interleukin RelatedBacterialILInhibitorinhibitorinhibit

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