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  3. Cetrimonium bromide

Cetrimonium bromide  (Synonyms: CTAB; Cetyltrimethylammonium bromide; Hexadecyltrimethylammonium bromide)

Cat. No.: HY-B1260 Purity: ≥98.0%
COA Handling Instructions

Cetrimonium bromide (CTAB), a quaternary ammonium, is an orally active cationic surfaetant. Cetrimonium bromide has toxicity and anticancer effect. Cetrimonium bromide inhibits cell migration and invasion through modulating the canonical and non-canonical TGF-β signaling pathways. Cetrimonium bromide can be used for DNA extraction.

For research use only. We do not sell to patients.

Cetrimonium bromide Chemical Structure

Cetrimonium bromide Chemical Structure

CAS No. : 57-09-0

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Solid + Solvent
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Cetrimonium bromide:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Cetrimonium bromide

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cetrimonium bromide (CTAB), a quaternary ammonium, is an orally active cationic surfaetant. Cetrimonium bromide has toxicity and anticancer effect. Cetrimonium bromide inhibits cell migration and invasion through modulating the canonical and non-canonical TGF-β signaling pathways. Cetrimonium bromide can be used for DNA extraction[1][2][3][4].

IC50 & Target

MMP-9

 

MMP-2

 

In Vitro

Cetrimonium bromide (1-5 μM, 24 h) cannot affect growth of SK-HEP-1 cells[1].
Cetrimonium bromide (5 μM, 16-24 h) attenuates cellular migration and invasion of SK-HEP-1cells[1].
Cetrimonium bromide (5 μM, 24 h) inhibits the protein expression of MMP-2 and MMP-9, increases the protein expression of TIMP-1 and TIMP-2 and restrain the protein expression of Rac1, cdc42 and RhoA in SK-HEP-1cells[1].
Cetrimonium bromide (5 μM, 12-72 h) induces apoptosis in human Head and neck cancer (HNC) cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: SK-HEP-1 cells
Concentration: 1 μM, 2.5 μM, 5 μM
Incubation Time: 24 h
Result: Retained more than 90% cell viability at 24 hours.

Cell Cycle Analysis[2]

Cell Line: HNC cells
Concentration: 5 μM
Incubation Time: 12 h, 24 h, 48 h, 72 h
Result: Induced caspase activation.
Observed the activation of the caspase cascade and increased in a time-dependent manner.

Western Blot Analysis[1]

Cell Line: SK-HEP-1 cells
Concentration: 1 μM, 2.5 μM, 5 μM
Incubation Time: 24 h
Result: Significantly down-regulates MMP-2 and MMP-9 expressions in SK-HEP-1 cells in a dose-dependent manner.
Significantly increased the protein expression of TIMP-1 and TIMP-2.
Negatively affects the protein levels of Rac1, cdc42 and RhoA in SK-HEP-1 cells.
In Vivo

Cetrimonium bromide (5 mg/kg, Intraperitoneal injection, once a day for five consecutive days) ablated tumor-forming capacity of FaDu cells and delayed growth of established tumors in FaDu cells tumor-bearing mice[2].
Cetrimonium bromide (10-45 mg/kg, Supplemented in drinking water, once a day for 1 year) has subacute and chronic toxicity in the rat[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: FaDu cells tumor-bearing mice[2]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Induced a modest reduction in tumor development.
Delayed the mean time to reach a tumor-plus-leg diameters (TLD) of 14mm by ~3.7 days.
Animal Model: Sprague-Dawley rats[3]
Dosage: 10 mg/kg, 20 mg/kg, 45 mg/kg
Administration: Supplemented in drinking water
Result: Showed lower body weight at highest concentration within 3 weeks.
Reduced the body weight throughout the experimental in males and skeletal growth at highest dose level in both males and females.
Significantly decreased efficiency of food conversion in male rats receiving the highest dose.
Molecular Weight

364.45

Formula

C19H42BrN

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCC[N+](C)(C)C.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (27.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4.55 mg/mL (12.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7439 mL 13.7193 mL 27.4386 mL
5 mM 0.5488 mL 2.7439 mL 5.4877 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (2.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (137.19 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.7439 mL 13.7193 mL 27.4386 mL 68.5965 mL
5 mM 0.5488 mL 2.7439 mL 5.4877 mL 13.7193 mL
10 mM 0.2744 mL 1.3719 mL 2.7439 mL 6.8597 mL
DMSO 15 mM 0.1829 mL 0.9146 mL 1.8292 mL 4.5731 mL
20 mM 0.1372 mL 0.6860 mL 1.3719 mL 3.4298 mL
25 mM 0.1098 mL 0.5488 mL 1.0975 mL 2.7439 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cetrimonium bromide
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