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Sulfo-NHS-LC-LC-Biotin (Synonyms: Biotin-XX-SSE)

Cat. No.: HY-D1635
Handling Instructions

Sulfo-NHS-LC-LC-Biotin (Biotin-XX-SSE), a biotin reagent, is used to label the proteins exposed to the external leaflet of intact exosomes and contains a larger spacer arm between the biotin and amine reactive linker. The size of this linker helps to overcome steric hindrance and increases labeling efficiency at the crowded exosome surface.

For research use only. We do not sell to patients.

Sulfo-NHS-LC-LC-Biotin Chemical Structure

Sulfo-NHS-LC-LC-Biotin Chemical Structure

CAS No. : 194041-66-2

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Description

Sulfo-NHS-LC-LC-Biotin (Biotin-XX-SSE), a biotin reagent, is used to label the proteins exposed to the external leaflet of intact exosomes and contains a larger spacer arm between the biotin and amine reactive linker. The size of this linker helps to overcome steric hindrance and increases labeling efficiency at the crowded exosome surface[1][2].

In Vitro

Biotinylation of exosome proteins: Two hundred micrograms of intact exosomes were mixed with 10 mM Sulfo-NHS-LC-LC-Biotin at room temperature for 30 min. Four conditions were taken into account during this experiment: (a) an excess of Sulfo-NHS-LC-LC-Biotin was used to favor a complete saturation of exposed lysine residues and potential N-terminus, (b) the presence of the sulfonate group in Sulfo-NHS-LC-LC-Biotin blocks the reagent from penetrating the exosomal membrane, (c) Sulfo-NHS-LC-LC-Biotin has an spacer arm of 30.5 angstroms which improves the biotinylation of proteins in their natural conformation, and (d) amino acids labeled with Sulfo-NHS-LC-LC-Biotin will have an increase in mass of 452 Da. After incubation, the excess of Sulfo-NHS-LC-LC-Biotin was removed using a 10 KDa MWCO filtration device[2].
Rat aortic endothelial cells (RAEC) were surface modified in suspension with 1 mM Sulfo-NHS-LC-LC-biotin for 10 min, followed by pelleting and resuspension in PBS[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

669.74

Formula

C26H40N5NaO10S2

CAS No.
SMILES

O=C(C(CC1=O)S(=O)(O[Na])=O)N1OC(CCCCCNC(CCCCCNC(CCCC[[email protected]]2[[email protected]]3([H])[[email protected]](NC(N3)=O)([H])CS2)=O)=O)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Sulfo-NHS-LC-LC-Biotin
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HY-D1635
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